期刊
PLANTS-BASEL
卷 12, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/plants12102001
关键词
Berberis libanotica; phytochemicals; endemic plants; antioxidant activity; antibacterial activity; biological activities
This study investigated the phytochemical composition and biological activities of fruit and leaf extracts of Berberis libanotica, an endemic Lebanese plant. Various compounds were detected in the extracts, and the methanolic leaf extract showed the highest antioxidant capacity. The dichloromethane fruit extract reduced the activity of acetylcholinesterase and exhibited the best inhibition against certain cancer cells. The cyclohexane fruit and leaf extracts showed strong antibacterial effects.
Berberis libanotica Ehrenb. ex C.K. Schneider of the Berberidaceae family is an endemic Lebanese plant and is widely used in folk medicine. This study highlights the phytochemical composition and biological activities (in vitro) of fruit and leaf extracts. The two organs were extracted by cold maceration with four solvents of increasing polarity: cyclohexane, dichloromethane, ethyl acetate and methanol. The extracts were screened for their chemical composition by HPLC-DAD to identify and quantify the phenolic compounds. Volatile compounds were detected by GC-MS. The antioxidant capacity through DPPH inhibition was tested. The anti-acetylcholinesterase, antibacterial and anti-proliferative activities were evaluated. Thirteen compounds, including 12 phenolics, were detected in the fruits, whereas 8 phenolic compounds were identified in the leaves. A total of 137 volatile compounds were identified in both organs. At 50 mu g/mL, the methanolic leaf extract presented the highest antioxidant capacity, with an inhibition percentage of 54.9%. The dichloromethane fruit extract reduced the acetylcholinesterase activity by 65.3%. The cyclohexane leaf extract reduced the proliferation of the HCT-116 cells by 54.8%, while the dichloromethane fruit extract exhibited the best inhibition against the Caco-2 cells (54%). Interestingly, the minimum inhibitory concentration (MIC) value of the cyclohexane fruit extract against Salmonella enterica serovar Kentucky was 2.4 mu g/mL, and the MIC value of the cyclohexane leaf extract against E. coli was 9.7 mu g/mL.
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