期刊
ANTIBIOTICS-BASEL
卷 12, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/antibiotics12061016
关键词
beta-lactamases; AmpC; extended-spectrum beta-lactamase; carbapenemases; third-generation cephalosporin resistance; Ghana; Africa
Resistance to routine antimicrobials is observed in β-lactamase-producing Gram-negative bacteria (GNB) from clinical sources. The occurrence of carbapenemases, extended-spectrum β-lactamases (ESBLs), and AmpCs genes was also detected. Escherichia coli and Klebsiella pneumoniae were the most common GNB, with 46% and 17% prevalence respectively. Multi-drug resistance (MDR) was observed in 44% of the isolates.
Beta-lactamase (& beta;-lactamase)-producing Gram-negative bacteria (GNB) are of public health concern due to their resistance to routine antimicrobials. We investigated the antimicrobial resistance and occurrence of carbapenemases, extended-spectrum & beta;-lactamases (ESBLs) and AmpCs among GNB from clinical sources. GNB were identified using matrix-assisted laser desorption/ionization time of flight-mass spectrometry (MALDITOF-MS). Antimicrobial susceptibility testing was performed via Kirby-Bauer disk diffusion and a microscan autoSCAN system. & beta;-lactamase genes were determined via multiplex polymerase chain reactions. Of the 181 archived GNB analyzed, Escherichia coli and Klebsiella pneumoniae constituted 46% (n = 83) and 17% (n = 30), respectively. Resistance to ampicillin (51%), third-generation cephalosporins (21%), and ertapenem (21%) was observed among the isolates, with 44% being multi-drug resistant (MDR). & beta;-lactamase genes such as AmpCs ((bla(FOX-M) (64%) and bla(DHA-M) and bla(EDC-M) (27%)), ESBLs ((bla(CTX-M) (81%), other & beta;-lactamase genes bla(TEM) (73%) and bla(SHV) (27%)) and carbapenemase ((bla(OXA-)(48) (60%) and bla(NDM) and bla(KPC) (40%)) were also detected. One K. pneumoniae co-harbored AmpC (bla(FOX-M) and bla(EBC-M)) and carbapenemase (bla(KPC) and bla(OXA-)(48)) genes. bla(OXA-)(48) gene was detected in one carbapenem-resistant Acinetobacter baumannii. Overall, isolates were resistant to a wide range of antimicrobials including last-line treatment options. This underpins the need for continuous surveillance for effective management of infections caused by these pathogens in our settings.
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