4.6 Article

Screening of Klebsiella pneumoniae Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex® Superbug CRE and hvKp Assays

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Immunology

Changing Epidemiology of Carbapenemases Among Carbapenem-Resistant Enterobacterales From United States Hospitals and the Activity of Aztreonam-Avibactam Against Contemporary Enterobacterales (2019-2021)

Helio S. Sader et al.

Summary: As the prevalence of metallo-beta-lactamase (MBL)-producing Enterobacterales is increasing, there is an urgent need for effective antimicrobials. This study evaluated the activity of aztreonam-avibactam against 27,834 Enterobacterales isolates collected from US medical centers. The results showed that aztreonam-avibactam was highly effective, inhibiting over 99.9% of isolates at a concentration of <= 8 mg/L.

OPEN FORUM INFECTIOUS DISEASES (2023)

Review Infectious Diseases

Carbapenem-Resistant Klebsiella pneumoniae: Virulence Factors, Molecular Epidemiology and Latest Updates in Treatment Options

Theodoros Karampatakis et al.

Summary: Klebsiella pneumoniae is a Gram-negative opportunistic pathogen responsible for various infections. Carbapenem-resistant K. pneumoniae (CRKP) pose a major threat to public health, with high mortality rates in immunocompromised and critically ill patients. Understanding the virulence factors, molecular epidemiology, and treatment options for CRKP is crucial.

ANTIBIOTICS-BASEL (2023)

Article Infectious Diseases

Antimicrobial Susceptibility, Virulence, and Genomic Features of a Hypervirulent Serotype K2, ST65 Klebsiella pneumoniae Causing Meningitis in Italy

Aurora Piazza et al.

Summary: This study reports a hypermucoviscous K. pneumoniae strain causing recurrent meningitis and investigates its genomic features using whole-genome sequencing analysis. The strain is susceptible to most antibiotics despite the presence of some resistance genes and exhibits several virulence factors related to pathogenicity. Phylogenetic analysis shows high similarity between this strain and a Chinese isolate, suggesting a hidden circulation of this lineage.

ANTIBIOTICS-BASEL (2022)

Article Infectious Diseases

Resistome and virulome accretion in an NDM-1-producing ST147 sublineage of Klebsiella pneumoniae associated with an outbreak in Tuscany, Italy: a genotypic and phenotypic characterisation

Vincenzo Di Pilato et al.

Summary: This study investigated an outbreak of NDM-CPE in Tuscany, Italy, caused by ST147 K pneumoniae producing NDM-1. The clone had a complex resistome and virulome, capable of sustaining a large regional outbreak, highlighting the need for global surveillance programs to track and prevent their dissemination.

LANCET MICROBE (2022)

Article Infectious Diseases

Characterization of hypervirulent Klebsiella pneumoniae isolates in Belgium

Ahalieyah Anantharajah et al.

Summary: This study investigated 22 clinical invasive isolates of Klebsiella pneumoniae and found that hypervirulent strains showed increased pathogenicity. The majority of the isolates expressed the K2 capsular serotype and belonged to various MLST lineages, while the remaining strains expressed K1 and were associated with liver abscesses. Among the patients, 36% had primary extra-hepatic infections, 95% had sepsis, and the mortality rate was 30%. Improved clinical and microbiological diagnostics are needed to better understand and manage community-acquired invasive infections caused by hypervirulent K. pneumoniae.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2022)

Article Infectious Diseases

A geographic cluster of healthcare-associated carbapenemase-producing hypervirulent Klebsiella pneumoniae sequence type 23

Caoimhe Brennan et al.

Summary: This study describes the characteristics of carbapenemase-producing hypervirulent Klebsiella pneumoniae in a cluster in the South-East of Ireland, highlighting the risk it poses to healthcare and residential care populations. It identified 28 cases of hypervirulent K. pneumoniae and demonstrated its dissemination within healthcare facilities.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2022)

Article Infectious Diseases

Increase in NDM-1 and NDM-1/OXA-48-producing Klebsiella pneumoniae in Germany associated with the war in Ukraine, 2022

Mirco Sandfort et al.

Summary: German surveillance systems in 2022 have observed a rapid increase in the numbers of NDM-1 and NDM-1/OXA48 producing Klesbiella pneumoniae. Cases in Germany have shown a frequent presence in Ukraine before diagnosis. Whole genome sequencing of 200 isolates has revealed a high prevalence of sequence types ST147, ST307, ST395, and ST23, indicating clonal dissemination and onward transmission in Germany. Screening and isolating patients from Ukraine may help prevent further transmission.

EUROSURVEILLANCE (2022)

Article Infectious Diseases

Molecular Epidemiology of Antimicrobial Resistance, Virulence and Capsular Serotypes of Carbapenemase-Carrying Klebsiella pneumoniae in China

Lina Zhao et al.

Summary: This study analyzed genomic data of carbapenemase-carrying Klebsiella pneumoniae (KPN) strains in China, revealing rich diversity in sequence types and capsular serotypes. The study also found that carbapenemase has spread to hypervirulent KPN, mammals, poultry, and insects. The prevalence of different capsular serotypes has changed over time, with ST11 replacing KL47 as the dominant serotype. Different genic indicators can be used to differentiate between types of KPN.

ANTIBIOTICS-BASEL (2022)

Letter Infectious Diseases

Community-acquired hypervirulent Klebsiella pneumoniae invasive infection in critically ill patients who dramatically improved

O. Bernard de Lajartre et al.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2022)

Review Microbiology

Epidemiological characteristics and molecular evolution mechanisms of carbapenem-resistant hypervirulent Klebsiella pneumoniae

Yu-Ling Han et al.

Summary: CR-hvKP, a type of Klebsiella pneumoniae, exhibits both hypervirulence and carbapenem resistance phenotypes, causing severe infections and posing significant challenges to global public health. The evolutionary mechanisms include horizontal transfer of plasmids carrying resistance and virulence genes.

FRONTIERS IN MICROBIOLOGY (2022)

Article Immunology

Efficacy of Ceftazidime-avibactam Plus Aztreonam in Patients With Bloodstream Infections Caused by Metallo-β-lactamase-Producing Enterobacterales

Marco Falcone et al.

Summary: This study aimed to compare the outcomes of patients with MBL-producing Enterobacterales bloodstream infections treated with CAZ-AVI + ATM or other active antibiotics. The results showed that CAZ-AVI + ATM was associated with lower 30-day mortality, lower clinical failure at day 14, and shorter length of stay.

CLINICAL INFECTIOUS DISEASES (2021)

Article Infectious Diseases

First Report of an Extensively Drug-Resistant ST23 Klebsiella pneumoniae of Capsular Serotype K1 Co-Producing CTX-M-15, OXA-48 and ArmA in Spain

Marta Hernandez et al.

Summary: This study highlighted the identification of a Klebsiella pneumoniae isolate in Spain, belonging to clone ST23 and presenting capsular serotype K1, carrying bla(CTX-M-15), bla(OXA-48), and armA genes. It emphasized the importance of implementing new surveillance strategies to monitor the emergence and spread risk of such extensively drug-resistant and hypervirulent K. pneumoniae isolates.

ANTIBIOTICS-BASEL (2021)

Article Multidisciplinary Sciences

A genomic surveillance framework and genotyping tool for Klebsiella pneumoniae and its related species complex

Margaret M. C. Lam et al.

Summary: Kleborate is a tool designed for analyzing genomes of Klebsiella pneumoniae and its related species complex, aimed at supporting genomic surveillance and epidemiology in research, clinical, and public health settings. By applying Kleborate to analyze Klebsiella genomes, global trends in antimicrobial resistance and virulence can be highlighted as examples of what could be achieved by using this genomic framework within more systematic genomic surveillance efforts.

NATURE COMMUNICATIONS (2021)

Article Multidisciplinary Sciences

AMRFinderPlus and the Reference Gene Catalog facilitate examination of the genomic links among antimicrobial resistance, stress response, and virulence

Michael Feldgarden et al.

Summary: With the advancement of technology, in silico approaches to assessing AMR gene content have become possible. NCBI has developed a comprehensive AMR gene database and AMR gene detection tool, expanding the Reference Gene Catalog and releasing AMRFinderPlus to provide a more accurate means of identifying AMR genes and determining their relationship with phenotypes.

SCIENTIFIC REPORTS (2021)

Article Infectious Diseases

Whole genome sequencing to decipher the virulence phenotype of hypervirulent Klebsiella pneumoniae responsible for liver abscess, Marseille, France

Sophie Alexandra Baron et al.

Summary: Pyogenic liver abscess caused by hypervirulent Klebsiella pneumoniae is increasingly reported in France and Europe. Whole genome sequencing confirms the diagnosis of these hypervirulent strains. Prolonged antibiotic therapy is necessary for successful treatment.

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES (2021)

Article Biochemical Research Methods

Unicycler: Resolving bacterial genome assemblies from short and long sequencing reads

Ryan R. Wick et al.

PLOS COMPUTATIONAL BIOLOGY (2017)

Article Biochemical Research Methods

Trimmomatic: a flexible trimmer for Illumina sequence data

Anthony M. Bolger et al.

BIOINFORMATICS (2014)

Article Biochemical Research Methods

SPAdes: A New Genome Assembly Algorithm and Its Applications to Single-Cell Sequencing

Anton Bankevich et al.

JOURNAL OF COMPUTATIONAL BIOLOGY (2012)