期刊
FOODS
卷 12, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/foods12061152
关键词
ginseng; metabolites; internal extractive electrospray ionization mass spectrometry; sequential sampling; metabolite fingerprinting
In this study, an internal extractive electrospray ionization mass spectrometry (iEESI-MS) method was developed to analyze active metabolites in ginseng samples without pretreatment. A total of 44 metabolites, including 32 ginsenosides, 6 sugars, and 6 organic acids, were identified in the ginseng samples. The study demonstrated that iEESI-MS-based metabolite fingerprints can be used to successfully discriminate different cultivation conditions of ginseng, providing an alternative solution for quality identification of plant drugs.
Ginseng, a kind of functional food and medicine with high nutritional value, contains various pharmacological metabolites that influence human metabolic functions. Therefore, it is very important to analyze the composition and metabolites of ginseng. However, the analysis of active metabolites in ginseng samples usually involves various experimental steps, such as extraction, chromatographic separation, and characterization, which may be time-consuming and laborious. In this study, an internal extractive electrospray ionization mass spectrometry (iEESI-MS) method was developed to analyze active metabolites in ginseng samples with sequential sampling and no pretreatment. A total of 44 metabolites, with 32 ginsenosides, 6 sugars, and 6 organic acids, were identified in the ginseng samples. The orthogonal partial least-squares discriminant analysis (OPLS-DA) score plot showed a clear separation of ginseng samples from different origins, indicating that metabolic changes occurred under different growing conditions. This study demonstrated that different cultivation conditions of ginseng can be successfully discriminated when using iEESI-MS-based metabolite fingerprints, which provide an alternative solution for the quality identification of plant drugs.
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