期刊
FRONTIERS IN MARINE SCIENCE
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmars.2023.1139713
关键词
UVC-LED; aquaculture; bacteria disinfection; photoreactivation; dark repair
This study aimed to investigate the efficacy of UVC-LED on pathogenic bacteria and the factors influencing bacterial reactivation. The results showed that UVC-LED effectively inactivated bacteria, and the inactivation rate increased with increasing UVC-LED fluence. Adequate UVC-LED irradiation and avoidance of visible light after disinfection can effectively inhibit bacterial reactivation. The findings provide reference for UV disinfection in aquaculture.
Ultraviolet disinfection is an important method for controlling the large-scale outbreaks of diseases in aquaculture. As a novel and promising light source, ultraviolet light-emitting diode (UV-LED) has the advantages of safety, high efficiency and no environmental pollution risks. However, it remains unclear whether UV-LEDs can replace traditional UV light sources for aquaculture water treatment processes. Present study aimed to investigate the efficacy of UVC-LEDs (265 nm) on pathogenic bacteria, specifically Aeromonas salmonicida and Escherichia coli. The effects of UVC-LED dose, light conditions, and temperature on bacterial reactivation were also investigated. The results showed that exposure to UVC-LED effectively inactivated both types of bacteria. To achieve 4.5-log inactivation of A. salmonicida and E. coli, 24 mJ/cm(2) and 28 mJ/cm(2) UVC-LED irradiation were required, and the inactivation rate increased with increasing UVC-LED fluence. Both A. salmonicida and E. coli were revived after UVC-LED disinfection, and photoreactivation was significantly higher than dark reactivation. Bacterial reactivation rate due to high-dose UVC-LED treatment was significantly lower than that of low-dose. After 72 h of reactivation, photoreactivation and dark reactivation rates were 1 +/- 0.4% and 2.2 +/- 0.2%for A. salmonicida, and 0.02% and 0% for E. coli, respectively. Besides, the photoreactivation rates for the two bacteria exhibited different correlations with temperature. The highest photoreactivation rate for A. salmonicida was 68.7 +/- 4% at 20 degrees C, while the highest photoreactivation rate for E. coli was 53.98 +/- 2.9% at 15 degrees C for 48 h. This study reveals the rapid and efficient inactivation of bacteria by UVC-LED, and elucidates the mechanism and influencing factors for inactivation and reactivation by UVC-LED. The study also highlights that adequate UVC-LED irradiation and avoidance of visible light after UVC-LED disinfection can effectively inhibit bacterial reactivation. Our findings form a reference for the design and operation of UV disinfection in aquaculture.
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