4.7 Article

Reversible reprotoxic effects of manganese through DAF-16 transcription factor activation and vitellogenin downregulation in Caenorhabditis elegans

期刊

LIFE SCIENCES
卷 151, 期 -, 页码 218-223

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PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2016.03.016

关键词

Manganese; Vitellogenin; Caenorhabditis elegans; DAF-16 transcription factor; Brood size

资金

  1. Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq Universal) [472669/2011-7, 475896/2012-2]
  2. Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)
  3. Fundacao de Amparo a Pesquisa do Estado do Rio Grande do Sul (Programa de Apoio a Nucleos emergentes - PRONEM/FAPERGS) [11/2029-1]
  4. NIH [R01 ES10563]

向作者/读者索取更多资源

Aims: Vitellogenesis is the yolk production process which provides the essential nutrients for the developing embryos. Yolk is a lipoprotein particle that presents lipids and lipid-binding proteins, referred to as vitellogenins (VIT). The Caenorhabditis elegans nematode has six genes encoding VIT lipoproteins. Several pathways are known to regulate vitellogenesis, including the DAF-16 transcription factor. Some reports have shown that heavy metals, such as manganese (Mn), impair brood size in C. elegans; however the mechanisms associated with this effect have yet to be identified. Our aim was to evaluate Mn's effects on C. elegans reproduction and better understand the pathways related to these effects. Main methods.: Young adult larval stage worms were treated for 4 h with Mn in 85 mM NaCl and Escherichia coli OP50 medium. Key findings.: Mn reduced egg-production and egg-laying during the first 24 h after the treatment, although the total number of progenies were indistinguishable from the control group levels. This delay may have occurred due to DAF-16 activation, which was noted only after the treatment and was not apparent 24 h later. Moreover, the expression, protein levels and green fluorescent protein (GFP) fluorescence associated with VIT were decreased soon after Mn treatment and recovered after 24 h. Significance: Combined, these data suggest that the delay in egg-production is likely regulated by DAF-16 and followed by the inhibition of VIT transport activity. Further studies are needed to clarify the mechanisms associated with Mn-induced DAF-16 activation. (C) 2016 Elsevier Inc. All rights reserved.

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