4.6 Article

Dynamics of the Lipidome in a Colon Simulator

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METABOLITES
卷 13, 期 3, 页码 -

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MDPI
DOI: 10.3390/metabo13030355

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lipidomics; metabolomics; gut microbiome; in vitro colon simulator

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Current evidence suggests that gut microbiome-derived lipids play a crucial role in regulating host lipid metabolism. This study used targeted and untargeted lipidomics to analyze in vitro-derived feces and found that the lipid profiles of the simulated intestinal chyme were influenced by the vessel and time. Various types of lipids, including phosphatidylcholines, sphingomyelins, triacylglycerols, and endocannabinoids, were found to be altered in different compartments of the human colon model over time.
Current evidence suggests that gut microbiome-derived lipids play a crucial role in the regulation of host lipid metabolism. However, not much is known about the dynamics of gut microbial lipids within the distinct gut biogeographic. Here we applied targeted and untargeted lipidomics to in vitro-derived feces. Simulated intestinal chyme was collected from in vitro gut vessels (V1-V4), representing proximal to distal parts of the colon after 24 and 48 h with/without polydextrose treatment. In total, 44 simulated chyme samples were collected from the in vitro colon simulator. Factor analysis showed that vessel and time had the strongest impact on the simulated intestinal chyme lipid profiles. We found that levels of phosphatidylcholines, sphingomyelins, triacylglycerols, and endocannabinoids were altered in at least one vessel (V1-V4) during simulation. We also found that concentrations of triacylglycerols, diacylglycerols, and endocannabinoids changed with time (24 vs. 48 h of simulation). Together, we found that the simulated intestinal chyme revealed a wide range of lipids that remained altered in different compartments of the human colon model over time.

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