期刊
METABOLITES
卷 13, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/metabo13030425
关键词
Beauveria bassiana; polyketide synthase; nonribosomal peptides; GNPS; molecular networking
This study investigated the metabolite profiles of pks14 and pks15 gene overexpression strains in B. bassiana and compared them to their knockout strains using LC-MS/MS with GNPS. The results showed that pks14 and pks15 clusters interacted with biosynthetic clusters encoding insect-virulent metabolites and upregulated certain secondary metabolites. This suggests that pks14 and pks15 may be directly or indirectly associated with key pathways in insect pathogenesis of B. bassiana.
Beauveria bassiana is a globally distributed entomopathogenic fungus that produces various secondary metabolites to support its pathogenesis in insects. Two polyketide synthase genes, pks14 and pks15, are highly conserved in entomopathogenic fungi and are important for insect virulence. However, understanding of their mechanisms in insect pathogenicity is still limited. Here, we overexpressed these two genes in B. bassiana and compared the metabolite profiles of pks14 and pks15 overexpression strains to those of their respective knockout strains in culture and in vivo using tandem liquid chromatography-mass spectrometry (LC-MS/MS) with Global Natural Products Social Molecular Networking (GNPS). The pks14 and pks15 clusters exhibited crosstalk with biosynthetic clusters encoding insect-virulent metabolites, including beauvericins, bassianolide, enniatin A, and the intracellular siderophore ferricrocin under certain conditions. These secondary metabolites were upregulated in the pks14-overexpressing strain in culture and the pks15-overexpressing strain in vivo. These data suggest that pks14 and pks15, their proteins or their cluster components might be directly or indirectly associated with key pathways in insect pathogenesis of B. bassiana, particularly those related to secondary metabolism. Information about interactions between the polyketide clusters and other biosynthetic clusters improves scientific understanding about crosstalk among biosynthetic pathways and mechanisms of pathogenesis.
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