期刊
PATHOGENS
卷 12, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/pathogens12030479
关键词
Carbapenemase; Enterobacterales; ST307; COVID-19
类别
Between March 2020 and September 2021, a total of 82 clinical Enterobacterales isolates harboring bla(KPC) and MBL genes were confirmed. The CC307 and CC11 clones were found to be predominant, with 49.4% and 37.7% of K. pneumoniae isolates belonging to these clones, respectively. The combinations of bla(KPC) and bla(NDM) were the most common, with bla(KPC-2) plus bla(NDM-5) accounting for 55% of isolates. The highest susceptibility rates were observed for aztreonam/avibactam and aztreonam/relebactam.
Background. The global spread of carbapenemase-producing Enterobacterales has become an epidemiological risk for healthcare systems by limiting available antimicrobial treatments. The COVID-19 pandemic worsened this scenario, prompting the emergence of extremely resistant microorganisms. Methods. Between March 2020 and September 2021, the NRL confirmed 82 clinical Enterobacterales isolates harboring a combination of bla(KPC) and MBL genes. Molecular typing was analyzed by PFGE and MLST. Modified double-disk synergy (MDDS) tests were used for phenotypic studies. Results. Isolates were submitted from 28 hospitals located in seven provinces and Buenos Aires City, including 77 K. pneumoniae, 2 K. oxytoca, 2 C. freundii, and 1 E. coli. Almost half of K. pneumoniae isolates (n = 38; 49.4%), detected in 15 hospitals, belong to the CC307 clone. CC11 was the second clone, including 29 (37.7%) isolates (22, ST11 and 7, ST258) from five cities and 12 hospitals. Three isolates belonging to CC45 were also detected. The carbapenemase combinations observed were as follows: 55% bla(KPC-2) plus bla(NDM-5); 32.5% bla(KPC-2) plus bla(NDM-1;) 5% bla(KPC-3) plus bla(NDM-1;) 5% bla(KPC-2) plus bla(IMP-8); and 2.5% strain with bla(KPC-2) plus bla(NDM-5) plus bla(OXA-163). Aztreonam/avibactam and aztreonam/relebactam were the most active combinations (100% and 91% susceptible, respectively), followed by fosfomycin (89%) and tigecycline (84%). Conclusions. The MDDS tests using ceftazidime-avibactam/EDTA and aztreonam/boronic acid disks improved phenotypic classification as dual producers. The successful high-risk clones of K. pneumoniae, such as hyper-epidemic CC307 and CC11 clones, drove the dissemination of double carbapenemase-producing isolates during the COVID-19 pandemic.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据