期刊
FRONTIERS IN MOLECULAR BIOSCIENCES
卷 10, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2023.1017757
关键词
GAL4; cyclic peptide; combinatorial library; yeast two-hybrid; protein interference; galactose metabolism; drug discovery
Genetically-encoded combinatorial peptide libraries are useful for identifying peptides with potential therapeutic applications. In this study, a cyclic peptide called G4CP2 was identified and characterized. G4CP2 interferes with the function of GAL4 protein, a transcription factor involved in galactose metabolism in yeast. The results demonstrate the potential biotechnological applications of G4CP2 and the utility of CYCLIC as a tool for peptide identification.
Genetically-encoded combinatorial peptide libraries are convenient tools to identify peptides to be used as therapeutics, antimicrobials and functional synthetic biology modules. Here, we report the identification and characterization of a cyclic peptide, G4CP2, that interferes with the GAL4 protein, a transcription factor responsible for the activation of galactose catabolism in yeast and widely exploited in molecular biology. G4CP2 was identified by screening CYCLIC, a Yeast Two-Hybrid-based combinatorial library of cyclic peptides developed in our laboratory. G4CP2 interferes with GAL4-mediated activation of galactose metabolic enzymes both when expressed intracellularly, as a recombinant peptide, and when provided exogenously, as a chemically-synthesized cyclic peptide. Our results support the application of G4CP2 in microbial biotechnology and, additionally, demonstrate that CYCLIC can be used as a tool for the rapid identification of peptides, virtually without any limitations with respect to the target protein. The possible biotechnological applications of cyclic peptides are also discussed.
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