期刊
MICROORGANISMS
卷 11, 期 3, 页码 -出版社
MDPI
DOI: 10.3390/microorganisms11030692
关键词
diabetic; mupirocin; MRSA; crystal violet assay; modified Gram stain
类别
The study developed a simple and inexpensive method to induce biofilm formation in-vivo using Swiss albino mice for the evaluation of the antibiofilm activity of pharmacological agents. Preformed biofilm and MRSA culture were introduced into excision wounds of diabetic mice. The method successfully developed biofilm after 24 hours of incubation, which was confirmed by microscopic examination and a crystal violet assay. It was also effective in inducing profound infection and biofilm formation in the excision wounds within 72 hours.
The study developed a simple and inexpensive method to induce biofilm formation in-vivo for the evaluation of the antibiofilm activity of pharmacological agents using Swiss albino mice. Animals were made diabetic using streptozocin and nicotinamide. A cover slip containing preformed biofilm along with MRSA culture was introduced into the excision wound in these animals. The method was effective in developing biofilm on the coverslip after 24 h incubation in MRSA broth which was confirmed by microscopic examination and a crystal violet assay. Application of preformed biofilm along with microbial culture induced a profound infection with biofilm formation on excision wounds in 72 h. This was confirmed by macroscopic, histological, and bacterial load determination. Mupirocin, a known antibacterial agent effective against MRSA was used to demonstrate antibiofilm activity. Mupirocin was able to completely heal the excised wounds in 19 to 21 days while in the base-treated group, healing took place between 30 and 35 days. The method described is robust and can be reproduced easily without the use of transgenic animals and sophisticated methods such as confocal microscopy.
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