期刊
ANTIOXIDANTS
卷 12, 期 6, 页码 -出版社
MDPI
DOI: 10.3390/antiox12061190
关键词
marine-derived bioactive peptides; self-assembled peptides materials; wound healing; topical application; molecular docking
In recent years, peptide self-assembling materials have gained attention in various fields of study. This research focused on obtaining supramolecular peptide self-assembling materials (CAPs) from Pacific oysters via enzymatic hydrolysis. The study explored the pro-healing mechanisms of CAPs on skin wounds through in vitro and in vivo experiments, revealing their potential for traceless wound healing.
Peptide self-assembling materials have received significant attention from researchers in recent years, emerging as a popular field in biological, environmental, medical, and other new materials studies. In this study, we utilized controllable enzymatic hydrolysis technology (animal proteases) to obtain supramolecular peptide self-assembling materials (CAPs) from the Pacific oyster (Crassostrea gigas). We conducted physicochemical analyses to explore the pro-healing mechanisms of CAPs on skin wounds in both in vitro and in vivo experiments through a topical application. The results demonstrated that CAPs exhibit a pH-responsive behavior for self-assembly and consist of peptides ranging from 550 to 2300 Da in molecular weight, with peptide chain lengths of mainly 11-16 amino acids. In vitro experiments indicated that CAPs display a procoagulant effect, free radical scavenging activity, and promote the proliferation of HaCaTs (112.74% and 127.61%). Moreover, our in vivo experiments demonstrated that CAPs possess the ability to mitigate inflammation, boost fibroblast proliferation, and promote revascularization, which accelerates the epithelialization process. Consequently, a balanced collagen I/III ratio in the repaired tissue and the promotion of hair follicle regeneration were observed. With these remarkable findings, CAPs can be regarded as a natural and secure treatment option with high efficacy for skin wound healing. The potential of CAPs to be further developed for traceless skin wound healing is an exciting area for future research and development.
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