☆ 4.7 Review

Droplets microfluidics platform-A tool for single cell research

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2023)

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Summary: Complex coacervates are liquid-like droplets that lack membrane structure, limiting their colloidal stability. We developed caged-coacervates surrounded by a semipermeable shell of silica nanocapsules to enhance stability and regulate molecular transport. The biomimetic microreactors assembled using these caged-coacervates showed improved colloidal stability.

NANOSCALE (2023)

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Article Multidisciplinary Sciences

A home-made pipette droplet microfluidics rapid prototyping and training kit for digital PCR, microorganism/cell encapsulation and controlled microgel synthesis

Liao Chen et al.

Summary: This article introduces an inexpensive homemade pipette droplet microfluidics kit, which is suitable for design, training, and educational labs. The kit allows the generation of droplets with varying sizes and fluid properties, and can be used for applications such as PCR reactions, algal culturing, and synthesis of microgels.

SCIENTIFIC REPORTS (2023)

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Review Chemistry, Analytical

Advances in Microfluidics for Single Red Blood Cell Analysis

Georgii V. V. Grigorev et al.

Summary: The utilization of microfluidic chips for single RBC studies has attracted great interest in recent years. It involves filtering, trapping, analyzing, and releasing single erythrocytes for various applications. This article critically reviews the current state-of-the-art and recent advances in microfluidics for single RBC analyses, including integrated sensors, trapping arrays, dielectrophoretic and agglutination/aggregation studies, clinical implications, microarrays, sorting/counting and trapping techniques, organs-on-chips, multi-organ chips, and drug discovery. The limitations and future prospects of each technology are also addressed.

BIOSENSORS-BASEL (2023)

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Review Chemistry, Analytical

Droplet microfluidics for CTC-based liquid biopsy: a review

Lin Jiang et al.

Summary: Circulating tumor cells (CTCs) are important biomarkers in liquid biopsy, and their number and heterogeneity play a crucial role in cancer diagnosis and personalized medicine. However, due to their low abundance, conventional methods can only detect CTCs at the population level. Therefore, droplet microfluidics, as a high-throughput and sensitive single-cell analysis platform, becomes a significant tool for quantitative detection and heterogeneity analysis of CTCs.

ANALYST (2023)

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Review Cell Biology

A compendium of single extracellular vesicle flow cytometry

Joshua A. Welsh et al.

Summary: Flow cytometry (FCM) is able to characterize single extracellular vesicles (EVs), but EVs are more difficult to detect than cells because their signals overlap with background noise. To overcome this issue, the EV FCM working group published a framework (MIFlowCyt-EV) to improve the interpretation, reporting, and reproducibility of EV FCM experiments.

JOURNAL OF EXTRACELLULAR VESICLES (2023)

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Review Chemistry, Multidisciplinary

Combating Antimicrobial Resistance via Single-Cell Diagnostic Technologies Powered by Droplet Microfluidics

Kuangwen Hsieh et al.

Summary: Antimicrobial resistance is a global threat, and there is an urgent need for rapid identification and antimicrobial susceptibility testing technologies to combat the rise of multi-drug-resistant pathogens. Utilizing droplet microfluidics, high-sensitivity detection and rapid response to single cells can be achieved.

ACCOUNTS OF CHEMICAL RESEARCH (2022)

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Review Chemistry, Multidisciplinary

Droplet Microfluidics for High-Throughput Analysis of Antibiotic Susceptibility in Bacterial Cells and Populations

Witold Postek et al.

Summary: Antibiotic-resistant bacteria are a growing concern in various environments, including healthcare. Urgent action is needed to discover new antibiotics, diagnose resistance in infectious pathogens, and understand the distribution of resistance levels in populations and single cells. Droplet microfluidics offers high-throughput screening capabilities, allowing for the search of drug combinations to combat multidrug-resistant bacteria and the exploration of resistance origins. Manipulating aqueous droplets containing antibiotics and bacteria, microfluidic systems can automate the preparation of antibiotic concentration gradients for combinatorial assays.

ACCOUNTS OF CHEMICAL RESEARCH (2022)

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Article Chemistry, Analytical

Single-Cell Digital Microfluidic Mass Spectrometry Platform for Efficient and Multiplex Genotyping of Circulating Tumor Cells

Qingyu Ruan et al.

Summary: This study introduces a single-CTC mass spectrometry analysis method based on digital microfluidics, which can efficiently and accurately perform multiplex mutation profiling of individual CTCs, offering new avenues for cancer therapy guidance.

ANALYTICAL CHEMISTRY (2022)

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Review Immunology

Single-Cell Technologies for the Study of Antibody-Secreting Cells

Matteo Broketa et al.

Summary: Antibody-secreting cells (ASC), including plasmablasts and plasma cells, produce antibodies with different affinities. ASC are responsible for immediate humoral B cell response and the development of humoral memory and immunity. Studying the functions and transcriptional diversity of ASC is crucial for understanding the polyclonal humoral response.

FRONTIERS IN IMMUNOLOGY (2022)

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Review Chemistry, Analytical

Microfluidic-Based Droplets for Advanced Regenerative Medicine: Current Challenges and Future Trends

Hojjatollah Nazari et al.

Summary: Microfluidics is a promising approach for the fabrication of monodispersed droplets, offering potential solutions to the limitations of regenerative medicine. This technology has been applied in various fields such as stem cell therapy, tissue engineering, reproductive biology, and gene therapy.

BIOSENSORS-BASEL (2022)

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Article Chemistry, Multidisciplinary

Single-cell level point mutation analysis of circulating tumor cells through droplet microfluidics

Shihui Qiu et al.

Summary: Tumor heterogeneity is important for determining appropriate anticancer therapy. A new single-cell mutation detection platform based on droplet microfluidics has been developed, which offers high throughput, short experimental time, and simplified operations.

CHINESE CHEMICAL LETTERS (2022)

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Review Biochemical Research Methods

Application of microfluidic technology in antibody screening

Heng Sun et al.

Summary: Specific antibodies are widely used in the biomedical field. Current screening methods for specific antibodies, such as hybridoma technology and antibody engineering techniques, face challenges such as tedious processes and low efficiency. Microfluidics has been developed as a promising solution for high-throughput screening and high purity of antibodies.

BIOTECHNOLOGY JOURNAL (2022)

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Merits and advances of microfluidics in the pharmaceutical field: design technologies and future prospects

Amr Maged et al.

Summary: Microfluidics is a technique used to manipulate fluid flow in micro-channels for fabricating drug delivery vesicles of uniform size. Understanding the factors influencing nanoparticle formulation can guide the selection of proper microfluidic design and operating parameters. This review highlights the various microfluidic systems and parameters used to prepare and fine-tune nanoparticles, as well as the potential for scale-up production and preparing novel drug delivery systems using microfluidics.

DRUG DELIVERY (2022)

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Article Biotechnology & Applied Microbiology

Ultrahigh-throughput screening of industrial enzyme-producing strains by droplet-based microfluidic system

Huiling Yuan et al.

Summary: Droplet-based microfluidics has the potential to be a powerful tool for single-cell screening, but the accessibility of a droplet microfluidic high-throughput screening platform has been a limitation. In this study, researchers developed a microfluidic platform for high-throughput screening using fluorescence-activated droplet sorting technology. The platform allowed for encapsulation of single cells in droplets, cell growth and protein production, and sorting based on fluorescence intensities. The platform was successfully validated in experiments involving Bacillus strains and mutant libraries.

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY (2022)

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Leveraging single-cell sequencing to unravel intratumour heterogeneity and tumour evolution in human cancers

Amy L. Bowes et al.

Summary: This review provides an overview of current single-cell DNA sequencing technologies and their applications in studying intratumour heterogeneity (ITH) and tumour evolution. The advancements in methodological approaches have allowed for the exploration of evolutionary dynamics contributing to tumourigenesis, progression, metastasis, and therapy resistance. The use of in situ sequencing technologies and lineage tracing in both normal and malignant tissues are also discussed.

JOURNAL OF PATHOLOGY (2022)

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Microfluidic-assisted synthesis and modeling of stimuli-responsive monodispersed chitosan microgels for drug delivery applications

Omid Sartipzadeh et al.

Summary: This study highlights the significance of droplet microfluidic technology in the fields of chemical and biomedical engineering and provides a detailed description of the properties of chitosan droplets and their size and generation rate in different applications. Through experimental and simulation validation, the potential of this technology in drug delivery, tissue engineering, and biosensing is confirmed.

SCIENTIFIC REPORTS (2022)

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Fabrication of Polymer Microfluidics: An Overview

Yi-Je Juang et al.

Summary: Microfluidic platform technology provides a new strategy for the detection and analysis of analytes and biological entities by reducing dimensions. It enables the manipulation of particles through various forces and has become a popular choice for repeated short-time monitoring and affordable one-time usage with its diverse material properties and processing techniques.

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Alicia Cristina Pena-Romero et al.

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CANCERS (2022)

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High-throughput single-cell antibody secretion quantification and enrichment using droplet microfluidics-based FRET assay

Justina Rutkauskaite et al.

Summary: In this study, a droplet microfluidic approach was developed for high-throughput screening and sorting of antibody-secreting cells. The method utilizes Forster resonance electron transfer (FRET) signal to mediate the binding of antibodies and probes, enabling functional characterization of individual cells. The system efficiently screens antigen-specific cells and provides important data on antibody secretion rate and membrane-bound fraction.

ISCIENCE (2022)

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Microfluidic-Assisted Fabrication of Dual-Coated pH-Sensitive Mesoporous Silica Nanoparticles for Protein Delivery

Berrin Kucukturkmen et al.

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BIOSENSORS-BASEL (2022)

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Modular automated microfluidic cell culture platform reduces glycolytic stress in cerebral cortex organoids

Spencer T. Seiler et al.

Summary: Organ-on-a-chip systems are capable of culturing 3D organ-specific in vitro models that replicate the biology and physiology of their in vivo counterparts. This study introduces a multiplex platform for automating organoid cultures in isolated microenvironments, allowing for programming of different workflows and reagent reservoirs. RNA sequencing analysis shows that these automated cultures have benefits in reducing glycolytic and endoplasmic reticulum stress compared to conventional in vitro cultures.

SCIENTIFIC REPORTS (2022)

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A Review of Microfluidic Experimental Designs for Nanoparticle Synthesis

Adelina-Gabriela Niculescu et al.

Summary: This paper reviews recently developed microfluidic devices based on their geometry particularities, discussing the mixing and synthesis of nanoparticles within microchannels and exploring different chip configurations for nanomaterial fabrication.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

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Endothelial cell heterogeneity and microglia regulons revealed by a pig cell landscape at single-cell level

Fei Wang et al.

Summary: Using single-cell RNA sequencing, researchers generated a comprehensive map of single-cell transcriptomes from various organs of pigs. They identified a high degree of heterogeneity within cells and uncovered several functionally distinct cell types, including a subtype of endothelial cells in adipose tissues. Additionally, they predicted tissue- and cell type-specific communication between endothelial cells and other cell types through specific pathways. Furthermore, analysis of microglia transcriptomes across species revealed an evolutionally conserved regulon.

NATURE COMMUNICATIONS (2022)

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Three-Dimensional In Vitro Cell Culture Models for Efficient Drug Discovery: Progress So Far and Future Prospects

Shaimaa M. Badr-Eldin et al.

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Recent Advances in Drug Delivery System Fabricated by Microfluidics for Disease Therapy

Fuhao Jia et al.

Summary: Traditional drug therapy has limitations, but micro/nanoparticles can improve drug efficacy. Microfluidics has great potential in drug delivery and precision medicine. Well-designed microfluidic devices can fabricate multifunctional drug carriers, and the interaction of drugs with lymphatic vessels is often overlooked in organs-on-chips.

BIOENGINEERING-BASEL (2022)

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Microfluidic Formulation of Topological Hydrogels for Microtissue Engineering

Katarzyna O. Rojek et al.

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The immune system as a system of relations

Marc Daeron

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FRONTIERS IN IMMUNOLOGY (2022)

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Review Chemistry, Analytical

Application of Microfluidics in Drug Development from Traditional Medicine

Xue Li et al.

Summary: Traditional drug development strategies cannot meet the demand for drugs with low or no risk of side effects, highlighting the need for new development strategies. Microfluidics has played a crucial role in traditional medicine drug development, addressing many challenges. This review provides an overview of the applications of microfluidics in drug development, with a focus on its use in traditional medicine.

BIOSENSORS-BASEL (2022)

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Microfluidic-assisted fiber production: Potentials, limitations, and prospects

Afshin Abrishamkar et al.

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BIOMICROFLUIDICS (2022)

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A Detailed Overview About the Single-Cell Analyses of Solid Tumors Focusing on Colorectal Cancer

William J. Kothalawala et al.

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PATHOLOGY & ONCOLOGY RESEARCH (2022)

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The Promise of Single-cell Technology in Providing New Insights Into the Molecular Heterogeneity and Management of Acute Lymphoblastic Leukemia

Holly C. L. Pearson et al.

Summary: Single-cell sequencing provides detailed analysis of individual cell variation, offering new insights into the driving mechanisms of drug resistance and treatment failure in pediatric ALL. This technique improves clinical outcomes in ALL patients and has the potential to transform our understanding of the disease's fundamental biology.

HEMASPHERE (2022)

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Review Chemistry, Analytical

Single-cell droplet microfluidics for biomedical applications

Dan Liu et al.

Summary: Single-cell manipulation and analysis are crucial for studying biological processes and uncovering cellular heterogeneity. Microfluidic technologies offer a promising approach for this purpose. This article provides an overview of the technological development of single-cell droplet microfluidics, highlighting the dynamic parameters affecting droplet production and the geometric structures of microfluidic devices. The progress in passive and active droplet generation methods and various microfluidic tools for producing single-cell droplets and hydrogel microspheres is summarized. Furthermore, the popular applications of single-cell droplet microfluidics in biomedicine and the challenges that need to be overcome for future applications are discussed.

ANALYST (2022)

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Tumour-on-chip microfluidic platform for assessment of drug pharmacokinetics and treatment response

Tudor Petreus et al.

Summary: Petreus et al. introduce a novel tumour-on-chip microfluidic system that mimics pharmacokinetic profiles of compounds on 3D tumour spheroids to evaluate their response to treatments, successfully predicting efficacy from in vivo studies and guiding drug dose and schedules for optimal efficacy while reducing the need for animal studies.

COMMUNICATIONS BIOLOGY (2021)

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Machine learning enables design automation of microfluidic flow-focusing droplet generation

Ali Lashkaripour et al.

Summary: Microfluidic devices based on droplet generation show great potential in various life science applications, but the lack of predictive understanding makes the design process iterative and resource-intensive. The DAFD tool utilizes machine learning algorithms to predict performance and automate the design of flow-focusing droplet generators, reducing the need for design iterations.

NATURE COMMUNICATIONS (2021)

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Article Oncology

Multiregion single-cell sequencing reveals the transcriptional landscape of the immune microenvironment of colorectal cancer

Wei Wang et al.

Summary: This study utilized two single-cell RNA sequencing technologies to create an atlas of immune and nonimmune cells in colorectal cancer patients, revealing significant differences in the functions and states of B cells at different stages of tumor development. The research provides deeper insights into immune infiltration within CRC and suggests new directions for future immunotherapy research.

CLINICAL AND TRANSLATIONAL MEDICINE (2021)

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Review Biotechnology & Applied Microbiology

Microfluidics applications for high-throughput single cell sequencing

Wen-min Zhou et al.

Summary: The inherent heterogeneity of individual cells in cell populations plays significant roles in disease development and progression, with traditional gene profiling methods often masking such differences. Single cell sequencing and microfluidic technologies have emerged as frontiers in research, providing insights into individual cell differences and complex cell populations. The advantages and disadvantages of common high-throughput single cell sequencing technologies are discussed, along with brief illustrations of microfluidic applications in single cell sequencing for cancer and immune system disease diagnosis.

JOURNAL OF NANOBIOTECHNOLOGY (2021)

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Article Biotechnology & Applied Microbiology

Probing Single-Cell Macrophage Polarization and Heterogeneity Using Thermo-Reversible Hydrogels in Droplet-Based Microfluidics

B. M. Tiemeijer et al.

Summary: To study the biological function of adherent cell types at single-cell resolution with high throughput, combining droplet-based microfluidics with hydrogels is a versatile and powerful tool. It allows for robust droplet formation at low temperatures, catalyzer-free droplet gelation, and easy cell recovery after culture for downstream analysis.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2021)

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Droplet microfluidics on analysis of pathogenic microbes for wastewater-based epidemiology

Yangteng Ou et al.

Summary: The combination of wastewater-based epidemiology and droplet microfluidics is an effective method for monitoring public health, but requires more advanced analytical platforms. Research focuses on quantifying pathogen biomarkers in droplets, single-cell analysis in droplets, and the use of living cell biosensors in droplets.

TRAC-TRENDS IN ANALYTICAL CHEMISTRY (2021)

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Highly Efficient Transfection of Human Primary T Lymphocytes Using Droplet-Enabled Mechanoporation

Byeongju Joo et al.

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ACS NANO (2021)

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Microgel Single-Cell Culture Arrays on a Microfluidic Chip for Selective Expansion and Recovery of Colorectal Cancer Stem Cells

Dongguo Lin et al.

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Luyao Liu et al.

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Areti Strati et al.

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Microfluidic Encapsulation of Single Cells by Alginate Microgels Using a Trigger-Gellified Strategy

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BIOMICROFLUIDICS (2020)

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Joint single cell DNA-seq and RNA-seq of gastric cancer cell lines reveals rules of in vitro evolution

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LAB ON A CHIP (2020)

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Single-cell analysis: Advances and future perspectives

Emir Hodzic

BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES (2016)

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Review Immunology

Innate cell communication kick-starts pathogen-specific immunity

Amariliz Rivera et al.

NATURE IMMUNOLOGY (2016)

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Article Multidisciplinary Sciences

Longitudinal multiparameter assay of lymphocyte interactions from onset by microfluidic cell pairing and culture

Burak Dura et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016)

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Article Multidisciplinary Sciences

A microfluidic platform enabling single-cell RNA-seq of multigenerational lineages

Robert J. Kimmerling et al.

NATURE COMMUNICATIONS (2016)

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Article Immunology

Microchip screening Platform for single cell assessment of NK cell cytotoxicity

Karolin Guldevall et al.

FRONTIERS IN IMMUNOLOGY (2016)

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Review Cell Biology

Single Cell Isolation and Analysis

Ping Hu et al.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2016)

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Article Engineering, Biomedical

Controlled 3D culture in Matrigel microbeads to analyze clonal acinar development

Monika E. Dolega et al.

BIOMATERIALS (2015)

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Article Biotechnology & Applied Microbiology

Single-cell screening of photosynthetic growth and lactate production by cyanobacteria

Petter Hammar et al.

BIOTECHNOLOGY FOR BIOFUELS (2015)

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Article Biochemistry & Molecular Biology

Single-Cell Genomics Unveils Critical Regulators of Th17 Cell Pathogenicity

Jellert T. Gaublomme et al.

CELL (2015)

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Review Biochemistry & Molecular Biology

Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities

Kjetil Hodne et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2015)

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Article Multidisciplinary Sciences

High-throughput detection of ethanol-producing cyanobacteria in a microdroplet platform

Sara Abalde-Cela et al.

JOURNAL OF THE ROYAL SOCIETY INTERFACE (2015)

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Article Chemistry, Analytical

Development of a Microfluidic-Based Optical Sensing Device for Label-Free Detection of Circulating Tumor Cells (CTCs) Through Their Lactic Acid Metabolism

Tzu-Keng Chiu et al.

SENSORS (2015)

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Review Genetics & Heredity

Single-Cell Analysis in Cancer Genomics

Assieh Saadatpour et al.

TRENDS IN GENETICS (2015)

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Article Multidisciplinary Sciences

Ultrahigh-throughput discovery of promiscuous enzymes by picodroplet functional metagenomics

Pierre-Yves Colin et al.

NATURE COMMUNICATIONS (2015)

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Article Engineering, Biomedical

Microfluidic Generation of Monodisperse, Structurally Homogeneous Alginate Microgels for Cell Encapsulation and 3D Cell Culture

Stefanie Utech et al.

ADVANCED HEALTHCARE MATERIALS (2015)

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Article Biology

Digital signaling decouples activation probability and population heterogeneity

Ryan A. Kellogg et al.

ELIFE (2015)

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Article Biochemical Research Methods

Droplet optofluidic imaging for lambda-bacteriophage detection via co-culture with host cell Escherichia coli

J. Q. Yu et al.

LAB ON A CHIP (2014)

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Review Biochemical Research Methods

Microfluidic single-cell analysis for systems immunology

Michael Junkin et al.

LAB ON A CHIP (2014)

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Review Pharmacology & Pharmacy

Industrial lab-on-a-chip: Design, applications and scale-up for drug discovery and delivery

Goran T. Vladisavljevic et al.

ADVANCED DRUG DELIVERY REVIEWS (2013)

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Review Chemistry, Analytical

Analytical detection techniques for droplet microfluidics-A review

Ying Zhu et al.

ANALYTICA CHIMICA ACTA (2013)

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Article Biochemical Research Methods

Micro-optical lens array for fluorescence detection in droplet-based microfluidics

Jiseok Lim et al.

LAB ON A CHIP (2013)

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Article Biochemical Research Methods

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

Venkatachalam Chokkalingam et al.

LAB ON A CHIP (2013)

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Article Biochemical Research Methods

On-chip magnetic separation and encapsulation of cells in droplets

Aaron Chen et al.

LAB ON A CHIP (2013)

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Article Biochemical Research Methods

Single-cell analysis and sorting using droplet-based microfluidics

Linas Mazutis et al.

NATURE PROTOCOLS (2013)

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Article Chemistry, Analytical

Teaching Single-Cell Digital Analysis Using Droplet-Based Microfluidics

Majdi Najah et al.

ANALYTICAL CHEMISTRY (2012)

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Article Biochemistry & Molecular Biology

Picoliter Cell Lysate Assays in Microfluidic Droplet Compartments for Directed Enzyme Evolution

Balint Kintses et al.

CHEMISTRY & BIOLOGY (2012)

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Article Biochemical Research Methods

Monitoring the dynamics of primary T cell activation and differentiation using long term live cell imaging in microwell arrays

Irina Zaretsky et al.

LAB ON A CHIP (2012)

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Article Biochemical Research Methods

Highly sensitive and quantitative detection of rare pathogens through agarose droplet microfluidic emulsion PCR at the single-cell level

Zhi Zhu et al.

LAB ON A CHIP (2012)

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Article Multidisciplinary Sciences

Functional single-cell hybridoma screening using droplet-based microfluidics

Bachir El Debs et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

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Review Immunology

A deep profiler's guide to cytometry

Sean C. Bendall et al.

TRENDS IN IMMUNOLOGY (2012)

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Review Chemistry, Applied

Single-Cell Analysis in Biotechnology, Systems Biology, and Biocatalysis

Frederik S. O. Fritzsch et al.

ANNUAL REVIEW OF CHEMICAL AND BIOMOLECULAR ENGINEERING, VOL 3 (2012)

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Article Biochemical Research Methods

A microfluidic platform for on-demand formation and merging of microdroplets using electric control

Hao Gu et al.

BIOMICROFLUIDICS (2011)

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Article Biophysics

Droplet-based microfluidic platforms for single T cell secretion analysis of IL-10 cytokine

Tania Konry et al.

BIOSENSORS & BIOELECTRONICS (2011)

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Article Engineering, Chemical

Two-phase microfluidic flows

Chun-Xia Zhao et al.

CHEMICAL ENGINEERING SCIENCE (2011)

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Article Biochemical Research Methods

1-Million droplet array with wide-field fluorescence imaging for digital PCR

Andrew C. Hatch et al.

LAB ON A CHIP (2011)

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Article Multidisciplinary Sciences

Statistical Modeling of Single Target Cell Encapsulation

SangJun Moon et al.

PLOS ONE (2011)

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Review Chemistry, Multidisciplinary

Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology

Ashleigh B. Theberge et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2010)

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Article Surgery

Alginate Microencapsulation Technology for the Percutaneous Delivery of Adipose-Derived Stem Cells

Hunter R. Moyer et al.

ANNALS OF PLASTIC SURGERY (2010)

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Article Biochemical Research Methods

Agarose droplet microfluidics for highly parallel and efficient single molecule emulsion PCR

Xuefei Leng et al.

LAB ON A CHIP (2010)

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Article Multidisciplinary Sciences

Ultrahigh-throughput screening in drop-based microfluidics for directed evolution

Jeremy J. Agresti et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)

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Article Chemistry, Multidisciplinary

Bioinspired Nanocorals with Decoupled Cellular Targeting and Sensing Functionality

Liz Y. Wu et al.

SMALL (2010)

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Article Chemistry, Analytical

Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets

Yolanda Schaerli et al.

ANALYTICAL CHEMISTRY (2009)

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Review Chemistry, Multidisciplinary

Enzyme assays

Jean-Louis Reymond et al.

CHEMICAL COMMUNICATIONS (2009)

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Article Nanoscience & Nanotechnology

Microdroplet formation of water and nanofluids in heat-induced microfluidic T-junction

S. M. Sohel Murshed et al.

MICROFLUIDICS AND NANOFLUIDICS (2009)

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Article Nanoscience & Nanotechnology

Predictive model on micro droplet generation through mechanical cutting

Woon Seob Lee et al.

MICROFLUIDICS AND NANOFLUIDICS (2009)

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Article Biochemical Research Methods

Correlating short-term Ca2+ responses with long-term protein expression after activation of single T cells

Michael Kirschbaum et al.

LAB ON A CHIP (2009)

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Article Biochemical Research Methods

Fluorescence-activated droplet sorting (FADS): efficient microfluidic cell sorting based on enzymatic activity

Jean-Christophe Baret et al.

LAB ON A CHIP (2009)

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Article Chemistry, Multidisciplinary

Characterization of Emulsification at Flat Microchannel Y Junctions

Maartje L. J. Steegmans et al.

LANGMUIR (2009)

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Article Chemistry, Analytical

On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets

N. Reginald Beer et al.

ANALYTICAL CHEMISTRY (2008)

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Article Biochemistry & Molecular Biology

An integrated device for monitoring time-dependent in vitro expression from single genes in picolitre droplets

Fabienne Courtois et al.

CHEMBIOCHEM (2008)

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Correction Biochemistry & Molecular Biology

Droplet-based microfluidic platforms for the encapsulation and screening of mammalian cells and multicellular organisms (vol 15, pg 427, 2008)

Jenifer Clausell-Tormos et al.

CHEMISTRY & BIOLOGY (2008)

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Article Engineering, Electrical & Electronic

On-demand liquid-in-liquid droplet metering and fusion utilizing pneumatically actuated membrane valves

Bo-Chih Lin et al.

JOURNAL OF MICROMECHANICS AND MICROENGINEERING (2008)

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Article Biochemical Research Methods

Drop-based microfluidic devices for encapsulation of single cells

Sarah Koester et al.

LAB ON A CHIP (2008)

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Article Biochemical Research Methods

Controlled encapsulation of single-cells into monodisperse picolitre drops

Jon F. Edd et al.

LAB ON A CHIP (2008)

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Article Biochemical Research Methods

Biocompatible surfactants for water-in-fluorocarbon emulsions

C. Holtze et al.

LAB ON A CHIP (2008)

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Article Multidisciplinary Sciences

Microfluidic high-throughput encapsulation and hydrodynamic self-sorting of single cells

Max Chabert et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

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Article Multidisciplinary Sciences

Continuous inertial focusing, ordering, and separation of particles in microchannels

Dino Di Carlo et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

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Article Chemistry, Multidisciplinary

Monodisperse alginate hydrogel microbeads for cell encapsulation

Wei-Heong Tan et al.

ADVANCED MATERIALS (2007)

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Article Polymer Science

Exploring microfluidic routes to microgels of biological polymers

Hong Zhang et al.

MACROMOLECULAR RAPID COMMUNICATIONS (2007)

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Article Chemistry, Multidisciplinary

Quantitative detection of protein expression in single cells using droplet microfluidics

A. Huebner et al.

CHEMICAL COMMUNICATIONS (2007)

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Article Mechanics

Microscale tipstreaming in a microfluidic flow focusing device

Shelley L. Anna et al.

PHYSICS OF FLUIDS (2006)

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Article Multidisciplinary Sciences

Single-cell proteomic analysis of S-cerevisiae reveals the architecture of biological noise

John R. S. Newman et al.

NATURE (2006)

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Review Chemistry, Analytical

Micro total analysis systems. Latest advancements and trends

Petra S. Dittrich et al.

ANALYTICAL CHEMISTRY (2006)

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Article Biotechnology & Applied Microbiology

A microengraving method for rapid selection of single cells producing antigen-specific antibodies

J. Christopher Love et al.

NATURE BIOTECHNOLOGY (2006)

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Review Clinical Neurology

Immunopathogenesis and immunotherapy of multiple sclerosis

B Hemmer et al.

NATURE CLINICAL PRACTICE NEUROLOGY (2006)

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Article Biochemical Research Methods

Formation of droplets and bubbles in a microfluidic T-junction - scaling and mechanism of break-up

P Garstecki et al.

LAB ON A CHIP (2006)

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Article Engineering, Chemical

Preparation of highly monodisperse droplet in a T-junction microfluidic device

J. H. Xu et al.

AICHE JOURNAL (2006)

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Article Biochemical Research Methods

Manipulating the generation of Ca-alginate microspheres using microfluidic channels as a carrier of gold nanoparticles

Keng-Shiang Huang et al.

LAB ON A CHIP (2006)

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Article Multidisciplinary Sciences

Regulated cell-to-cell variation in a cell-fate decision system

A Colman-Lerner et al.

NATURE (2005)

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Article Chemistry, Physical

Droplet formation in a T-shaped microchannel junction:: A model system for membrane emulsification

S van der Graaf et al.

COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS (2005)

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Review Genetics & Heredity

Stochasticity in gene expression:: From theories to phenotypes

M Kærn et al.

NATURE REVIEWS GENETICS (2005)

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Article Physics, Applied

The generation of highly monodisperse droplets through the breakup of hydrodynamically focused microthread in a microfluidic device

QY Xu et al.

APPLIED PHYSICS LETTERS (2004)

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Article Chemistry, Multidisciplinary

Generation of uniform colloidal assemblies in soft microfluidic devices

GR Yi et al.

ADVANCED MATERIALS (2003)

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Article Biochemistry & Molecular Biology

11-color, 13-parameter flow cytometry: Identification of human naive T cells by phenotype, function, and T-cell receptor diversity

SC De Rosa et al.

NATURE MEDICINE (2001)

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Review Multidisciplinary Sciences

Industrial biocatalysis today and tomorrow

A Schmid et al.

NATURE (2001)

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Article Multidisciplinary Sciences

Monolithic microfabricated valves and pumps by multilayer soft lithography

MA Unger et al.

SCIENCE (2000)

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Article Chemistry, Multidisciplinary

Monodisperse emulsion generation via drop break off in a coflowing stream

PB Umbanhowar et al.

LANGMUIR (2000)

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