期刊
LEUKEMIA
卷 31, 期 2, 页码 350-360出版社
SPRINGERNATURE
DOI: 10.1038/leu.2016.217
关键词
-
资金
- National Cancer Institute [CA170006]
- CLL Global Research Foundation
Previously, we reported that B-cell chronic lymphocytic leukemia (CLL) patients contained elevated levels of microvesicles (MVs). However, given the quiescent nature of CLL B-cells and the relative indolence of the disease, the dynamics of MV generation and their unique phenotypes are not clearly defined. In this study, we find that CLL B-cells generate MVs spontaneously and can be further induced by B-cell receptor-ligation. Most interestingly, CLL B-cells predominantly generate CD52(+) MVs, but not CD19(+) MVs in vitro, suggesting preferential usage of CD52 into leukemic-MVs and that the CLL plasma MV phenotypes corroborate well with the in vitro findings. Importantly, we detected increased accumulation of CD52(+) MVs in previously untreated CLL patients with progressive disease. Finally, sequential studies on MVs in pre- and post-therapy CLL patients demonstrate that although the plasma CD52(+) MV levels drop significantly after therapy in most and remain at low levels in some patients, a trend of increased accumulation of CD52(+) MVs was detected in majority of post-therapy CLL patients (25 of 33). In total, this study emphasizes that dynamic accumulation of CD52(+) MVs in plasma can be used to study CLL progression and may be a useful biomarker for patients as they progress and require therapy.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据