4.7 Article

Characterization of Bifidobacterium kashiwanohense that utilizes both milk- and plant-derived oligosaccharides

期刊

GUT MICROBES
卷 15, 期 1, 页码 -

出版社

TAYLOR & FRANCIS INC
DOI: 10.1080/19490976.2023.2207455

关键词

Bifidobacterium kashiwanohense; xylan; human milk oligosaccharide; plant-derived carbohydrate; extracellular xylanase; ABC transporter SBP

向作者/读者索取更多资源

Bifidobacterium kashiwanohense strains can utilize both milk- and plant-derived carbohydrates, including long-chain xylans and human milk oligosaccharides. This study identified key genetic factors that contribute to their ability to assimilate various carbohydrates and revealed their prevalence in the gut of not only infants, but also adults and weaning children worldwide.
Bifidobacteria are prominent members of the human gut microbiota throughout life. The ability to utilize milk- and plant-derived carbohydrates is important for bifidobacterial colonization of the infant and adult gut. The Bifidobacterium catenulatum subspecies kashiwanohense (B. kashiwanohense) was originally isolated from infant feces. However, only a few strains have been described, and the characteristics of this subspecies have been poorly investigated. Here, we characterized genotypes and phenotypes of 23 B. kashiwanohense-associated strains, including 12 newly sequenced isolates. Genome-based analysis clarified the phylogenetic relationship between these strains, revealing that only 13 strains are genuine B. kashiwanohense. We defined specific marker sequences and investigated the worldwide prevalence of B. kashiwanohense based on metagenome data. This revealed that not only infants but also adults and weaning children harbor this subspecies in the gut. Most B. kashiwanohense strains utilize long-chain xylans and possess genes for extracellular xylanase (GH10), arabinofuranosidase and xylosidase (GH43), and ABC transporters that contribute to the utilization of xylan-derived oligosaccharides. We also confirmed that B. kashiwanohense strains utilize short- and long-chain human milk oligosaccharides and possess genes for fucosidase (GH95 and GH29) and specific ABC transporter substrate-binding proteins that contribute to the utilization of a wide range of human milk oligosaccharides. Collectively, we found that B. kashiwanohense strains utilize both plant- and milk-derived carbohydrates and identified key genetic factors that allow them to assimilate various carbohydrates.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据