期刊
GUT MICROBES
卷 15, 期 1, 页码 -出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/19490976.2023.2205425
关键词
Crohn's disease; proteolytic activity; inflammation; DSS-induced colitis; Proteinase-activated receptor 2 (PAR2); gnotobiotic mice
Emerging evidence suggests that microbial proteolytic activity is implicated in ulcerative colitis (UC), but its role in Crohn's disease (CD) remains unclear. This study investigated the effects of colonizing germ-free mice with CD microbiota and healthy control microbiota. It was found that CD proteolytic microbiota increased colitis severity through a PAR2 pathway. These findings highlight the proinflammatory nature of CD proteolytic microbiota and its role in exacerbating colitis.
Emerging evidence implicates microbial proteolytic activity in ulcerative colitis (UC), but whether it also plays a role in Crohn's disease (CD) remains unclear. We investigated the effects of colonizing adult and neonatal germ-free C57BL/6 mice with CD microbiota, selected based on high (CD-HPA) or low fecal proteolytic activity (CD-LPA), or microbiota from healthy controls with LPA (HC-LPA) or HPA (HC-HPA). We then investigated colitogenic mechanisms in gnotobiotic C57BL/6, and in mice with impaired Nucleotide-binding Oligomerization Domain-2 (NOD2) and Protease-Activated Receptor 2 (PAR2) cleavage resistant mice (Nod2 (-/-); R38E-PAR2 respectively). At sacrifice, total fecal proteolytic, elastolytic, and mucolytic activity were analyzed. Microbial community and predicted function were assessed by 16S rRNA gene sequencing and PICRUSt2. Immune function and colonic injury were investigated by inflammatory gene expression (NanoString) and histology. Colonization with HC-LPA or CD-LPA lowered baseline fecal proteolytic activity in germ-free mice, which was paralleled by lower acute inflammatory cell infiltrate. CD-HPA further increased proteolytic activity compared with germ-free mice. CD-HPA mice had lower alpha diversity, distinct microbial profiles and higher fecal proteolytic activity compared with CD-LPA. C57BL/6 and Nod2 (-/-) mice, but not R38E-PAR2, colonized with CD-HPA had higher colitis severity than those colonized with CD-LPA. Our results indicate that CD proteolytic microbiota is proinflammatory, increasing colitis severity through a PAR2 pathway.
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