期刊
LEUKEMIA
卷 31, 期 5, 页码 1145-1153出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/leu.2016.321
关键词
-
资金
- Russian Science Foundation [14-35-00105, 15-15-00178]
- charitable fund 'Podari Zhizn'
- [MK-4583.2015.4]
- Russian Science Foundation [14-35-00105, 15-15-00178] Funding Source: Russian Science Foundation
alpha beta T-cell-depleted allogeneic hematopoietic cell transplantation holds promise for the safe and accessible therapy of both malignant and non-malignant blood disorders. Here we employed molecular barcoding normalized T-cell receptor (TCR) profiling to quantitatively track T-cell immune reconstitution after TCR alpha beta-/CD19-depleted transplantation in children. We demonstrate that seemingly early reconstitution of alpha beta T-cell counts 2 months after transplantation is based on only several hundred rapidly expanded clones originating from non-depleted graft cells. In further months, frequency of these hyperexpanded clones declines, and after 1 year the observed T-cell counts and TCR beta diversity are mostly provided by the newly produced T cells. We also demonstrate that high TCR beta diversity at day 60 observed for some of the patients is determined by recipient T cells and intrathymic progenitors that survived conditioning regimen. Our results indicate that further efforts on optimization of TCR alpha beta-/CD19-depleted transplantation protocols should be directed toward providing more efficient T-cell defense in the first months after transplantation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据