期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 63, 期 6, 页码 458-465出版社
OXFORD UNIV PRESS
DOI: 10.1111/lam.12667
关键词
Aspergillus carbonarius; Aspergillus niger; Aspergillus tubingensis; Aspergillus welwitschiae; droplet digital PCR; fungal ecology; mycotoxins; vineyards
资金
- U.S. Department of Agriculture Agricultural Research Service CRIS Project [2030-42000-038-00D]
Identification of populations of Aspergillus section Nigri species in environmental samples using traditional methods is laborious and impractical for large numbers of samples. We developed species-specific primers and probes for quantitative droplet digital PCR (ddPCR) to improve sample throughput and simultaneously detect multiple species in each sample. The ddPCR method was used to distinguish Aspergillus niger, Aspergillus welwitschiae, Aspergillus tubingensis and Aspergillus carbonarius in mixed samples of total DNA. Relative abundance of each species measured by ddPCR agreed with input ratios of template DNAs. Soil samples were collected at six time points over two growing seasons from two raisin vineyards in Fresno County, California. Aspergillus section Nigri strains were detected in these soils in the range of 10(2)-10(5)CFUg(-1). Relative abundance of each species varied widely among samples, but in 52 of 60 samples, A.niger was the most abundant species, ranging from 38 to 88% of the total population. In combination with total plate counts, this ddPCR method provides a high-throughput method for describing population dynamics of important potential mycotoxin-producing species in environmental samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据