Transcriptome Sequence Analysis of Defense Response of Resistant and Susceptible Bottle Gourd to Powdery Mildew

Powdery mildew (PM) is the main disease that afflicts bottle gourd. Previous studies on PM mainly focused on its effects on pumpkin, melon, and other crops; however, the exact molecular mechanism of bottle gourd resistance to PM remains unclear. RNA sequencing (RNA-Seq) technology was used to investigate the dynamic changes in leaf transcriptome profiles between resistant and susceptible gourd at 12, 24, 48, and 72 h post-inoculation with powdery mildew. Compared with a susceptible variety (G3), the expression levels of the differentially expressed genes of phenylpropanoid biosynthesis, starch, and sucrose metabolism, and plant-pathogen interaction pathways in disease-resistant plants were upregulated. We propose that disease resistance and tolerance in bottle gourd are enhanced via several pathways, including the antioxidant system, phenylalanine biosynthesis, and cell wall cellulose synthesis. Our research will provide an important basis for further screening and breeding PM resistance in bottle gourd.

Automated summary

RNA sequencing technology was used to investigate the changes in leaf transcriptome profiles of bottle gourd after inoculation with powdery mildew. Several metabolic pathways related to disease resistance were upregulated in the resistant plants, including phenylpropanoid biosynthesis, starch and sucrose metabolism, and plant-pathogen interaction. Enhancing these pathways can improve the resistance and tolerance of bottle gourd to powdery mildew, providing a basis for further breeding for resistance.