Efficient Micropropagation of Genetically Stable Panax ginseng Meyer by Somatic Embryogenesis

Panax ginseng Meyer is a valuable medicinal crop. However, the species' propagation is limited by its long reproductive cycle and low seed yield. The present study focused on P. ginseng plant regeneration via somatic embryogenesis and evaluated the genetic stability of regenerated plantlets. We assessed the effects of carbon source type and concentration on somatic embryo induction, maturation, and germination. Somatic embryogenesis was optimal in Murashige and Skoog (MS) medium supplemented with 5% sucrose; however, maturation peaked in 1/2 MS containing low concentrations of sucrose ranging from 1 to 2%. Germination and plant regeneration were optimal in germination medium supplemented with 2% sucrose based on high germination rates, efficient plantlet production, and balanced growth characteristics. Molecular marker analysis suggested that the genetic fidelity of the regenerated plants was comparable with that of the control. High-performance liquid chromatography (HPLC) analysis showed that in vitro-grown roots (IGRs) accumulated more ginsenoside than those of the control, but the ginsenoside content of 2 year old IGRs was similar to that of the controls after acclimatization. Our study provides valuable insights into the optimization of genetically stable micropropagation and could promote the distribution of superior P. ginseng cultivars with high product yields and quality.

Automated summary

This study investigated the regeneration of Panax ginseng through somatic embryogenesis and evaluated the genetic stability of the regenerated plantlets. The results showed that MS medium supplemented with 5% sucrose was optimal for somatic embryo induction, while 1/2 MS medium containing low concentrations of sucrose ranging from 1 to 2% was optimal for maturation. Germination and plant regeneration were optimal in germination medium supplemented with 2% sucrose. Molecular marker analysis indicated that the regenerated plants had comparable genetic fidelity with the control. This study provides valuable insights into the optimization of genetically stable micropropagation of P. ginseng.