☆ 4.7 Review

Transgene-Free Genome Editing for Biotic and Abiotic Stress Resistance in Sugarcane: Prospects and Challenges

AGRONOMY-BASEL (2023)

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The allene oxide synthase gene family in sugarcane and its involvement in disease resistance

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Summary: In this study, eight SsAOS and 36 SoAOS genes were identified in the genomes of sugarcane wild and tropical species. Evolutionary analysis showed that the AOS gene family was relatively conserved in structure but had diverse evolutionary mechanisms. Expression pattern and cis-regulatory element analysis revealed that SsAOSs were involved in various defensive responses. Furthermore, stable overexpression of the ShAOS1 gene enhanced the resistance of transgenic N. benthamiana plants to Fusarium solani var. coeruleum.

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Article Biotechnology & Applied Microbiology

Heritable transgene-free genome editing in plants by grafting of wild-type shoots to transgenic donor rootstocks

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Summary: Generation of transgene-free plant lines in one generation without the need for transgene elimination, culture recovery and selection, or use of viral editing vectors has been achieved by designing fusions of Cas9 and guide RNA transcripts to tRNA-like sequence motifs that facilitate RNA movement between transgenic rootstocks and wild-type scions. The graft-mobile gene editing system has been successfully demonstrated in Arabidopsis thaliana and Brassica rapa, and may have broad applications in breeding programs and crop plants.

NATURE BIOTECHNOLOGY (2023)

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Article Biochemistry & Molecular Biology

Highly efficient transgene-free genome editing in tobacco using an optimized CRISPR/Cas9 system, pOREU3TR

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Summary: CRISPR/Cas9 genome-editing technology has revolutionized plant science, showing great potential for crop improvement. By editing the NtPDS gene in tobacco, researchers have demonstrated that incorporating an OsU3-tRNA promoter combination into the CRISPR/Cas9 system can enhance editing efficiency. The optimized tobacco CRISPR/Cas9 system, named pOREU3TR, successfully produced transgene-free and homozygous gene-edited plants with significant differences in plant height and amino acid content. This OsU3-tRNA-sgRNA/AtUb10-Ros1 system provides essential improvements for increasing the efficiency of plant genome editing.

PLANT SCIENCE (2023)

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Article Plant Sciences

An increasing number of countries regulate genome editing in crops

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NEW PHYTOLOGIST (2023)

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Article Plant Sciences

Tools and targets: The dual role of plant viruses in CRISPR-Cas genome editing

Mireia Uranga et al.

Summary: Using plant RNA viruses as transient delivery vectors is an improved method for CRISPR-Cas genome editing, allowing for rapid editing in a short period of time. This method shows potential for enhancing plant resistance using CRISPR-Cas technology. However, there are still challenges and limitations to be addressed.

PLANT GENOME (2023)

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Article Biochemistry & Molecular Biology

Genome-wide characterization of sugarcane catalase gene family identifies a ScCAT1 gene associated disease resistance

Qibin Wu et al.

Summary: In this study, 16 SsCAT genes were identified in Saccharum plants and shown to play important roles in plant growth, development, hormone response, and stress tolerance. The ScCAT1 gene was found to be up-regulated under S. scitamineum stress and its overexpression in Nicotiana benthamiana enhanced resistance to pathogen infection through scavenging of excessive ROS and up-regulating genes related to HR, ROS, and SA pathways.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2023)

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Article Plant Sciences

Heritable DNA-free genome editing of canola (Brassica napus L.) using PEG-mediated transfection of isolated protoplasts

Vladimir Sidorov et al.

Summary: Efficient and high-throughput system for protoplast isolation, DNA-free editing, and production of edited canola plants has been developed. The use of ribonucleoproteins (RNPs) for editing in protoplasts led to approximately 40% editing frequency, with successful edits found in callus colonies and regenerated plants, demonstrating the heritability of the edits.

IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT (2022)

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Article Biochemistry & Molecular Biology

Genome editing of indica rice ASD16 for imparting resistance against rice tungro disease

Yaiphabi Kumam et al.

Summary: This study successfully used CRISPR/Cas9 technology to edit the genome of indica rice, creating novel mutations that confer resistance to rice tungro disease (RTD). These mutations specifically affect the target residue and can be faithfully inherited to subsequent generations.

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Article Biotechnology & Applied Microbiology

Strong and tunable anti-CRISPR/Cas activities in plants

Camilo Calvache et al.

Summary: This study demonstrates that anti-CRISPR proteins AcrIIA4 and AcrVA1 can prevent CRISPR/Cas-mediated gene editing in plants, opening up new possibilities for customized control of gene editing and gene expression.

PLANT BIOTECHNOLOGY JOURNAL (2022)

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Article Biotechnology & Applied Microbiology

Engineering of rice varieties with enhanced resistances to both blast and bacterial blight diseases via CRISPR/Cas9

Yanbiao Zhou et al.

Summary: In this study, we have developed mutants with resistance to rice blast and bacterial blight using CRISPR/Cas9 gene editing technology. The mutants showed strong resistance to the diseases and did not trade-off with main agricultural traits.

PLANT BIOTECHNOLOGY JOURNAL (2022)

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Letter Biotechnology & Applied Microbiology

Editing gene families by CRISPR/Cas9: accelerating the isolation of multiple transgene-free null mutant combinations with much reduced labor-intensive analysis

Ning Yang et al.

PLANT BIOTECHNOLOGY JOURNAL (2022)

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Article Plant Sciences

The rice heavy-metal transporter OsNRAMP1 regulates disease resistance by modulating ROS homoeostasis

Chuanliang Chu et al.

Summary: The OsNRAMP1 gene plays an important role in regulating metal ion and reactive oxygen species homeostasis in rice. Removal of this gene leads to enhanced broad-spectrum disease resistance and safety against toxic heavy metals.

PLANT CELL AND ENVIRONMENT (2022)

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Article Agronomy

Gene Editing Technologies for Sugarcane Improvement: Opportunities and Limitations

Chakravarthi Mohan et al.

Summary: Plant-based biofuels offer a promising alternative to depleting non-renewable fuel resources, with sugarcane being a key bioenergy crop. Researchers are focused on improving sugarcane biomass yield and quality through various biotechnological tools, particularly highlighting the cost-effective and versatile CRISPR-Cas9 technology for gene manipulation. The potential of genome editing technology in enhancing sugarcane for biofuel production, focusing on lignin modification, is also discussed, with a comparison of RNAi and TALEN techniques.

SUGAR TECH (2022)

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Review Biochemistry & Molecular Biology

A Comprehensive Review on the Heavy Metal Toxicity and Sequestration in Plants

Riyazuddin Riyazuddin et al.

Summary: Heavy metal toxicity poses a serious threat to the environment and human health. Plants suffer from inhibition of photosynthesis under heavy metal toxicity. Plants employ various strategies to mitigate the negative impact of heavy metal toxicity.

BIOMOLECULES (2022)

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Article Biochemistry & Molecular Biology

Overexpression of Sugarcane ScDIR Genes Enhances Drought Tolerance in Nicotiana benthamiana

Xiufang Li et al.

Summary: This study identified specific DIR genes in sugarcane that play a role in drought response and tolerance, and confirmed their function through heterologous expression in tobacco plants.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

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Article Plant Sciences

Tomato brown rugose fruit virus resistance generated by quadruple knockout of homologs of TOBAMOVIRUS MULTIPLICATION1 in tomato

Masayuki Ishikawa et al.

Summary: In this study, targeted mutagenesis of four susceptible genes in tomato using CRISPR/Cas9 technology conferred strong resistance against the emerging virus ToBRFV. The quadruple-mutant plants showed highly durable resistance and also exhibited resistance to other tobamovirus species. This provides a valuable genetic resource for future disease-resistant tomato breeding.

PLANT PHYSIOLOGY (2022)

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Article Microbiology

CRISPR-Cas9 Targeting of the eIF4E1 Gene Extends the Potato Virus Y Resistance Spectrum of the Solanum tuberosum L. cv. Desiree

Alessandra Lucioli et al.

Summary: This study demonstrates that CRISPR-Cas9-mediated knockout of the eIF4E1 gene can be used to broaden the resistance spectrum of potato to Potato virus Y (PVY) strains. The knockout is specific to eIF4E1 and does not affect its paralog, eIF4E2.

FRONTIERS IN MICROBIOLOGY (2022)

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Review Agronomy

Breeding for Drought Tolerance in Sugarcane: Indian Perspective

A. K. Mall et al.

Summary: Drought is a common abiotic disaster in India, causing significant impacts on sugarcane production. Sugarcane exhibits various adaptations and reactions to cope with drought stress, and several drought-tolerant genotypes and potential genes have been identified. Transgenic approaches have also been successful in developing drought-tolerant sugarcane in India.

SUGAR TECH (2022)

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Article Green & Sustainable Science & Technology

Development of Novel Rice Germplasm for Salt-Tolerance at Seedling Stage Using CRISPR-Cas9

Xiaoli Han et al.

Summary: In this study, CRISPR/Cas9 technology was used to edit the main effect gene OsRR22 in rice, resulting in a new germplasm with OsRR22 gene knockout. The mutant lines showed significant differences in plant growth and biomass compared to the wildtype after salt treatment, indicating that the OsRR22 mutation may improve salt tolerance in rice.

SUSTAINABILITY (2022)

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Article Plant Sciences

CRISPR/Cas9 Mediated Knockout of the OsbHLH024 Transcription Factor Improves Salt Stress Resistance in Rice (Oryza sativa L.)

Mohammad Shah Alam et al.

Summary: The study used the CRISPR/Cas9 strategy to investigate the role of OsbHLH024 in rice under salt stress. It was found that OsbHLH024 may play a negative regulatory role in salt stress, helping to better understand the molecular basis of rice production improvement under salt stress.

PLANTS-BASEL (2022)

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Article Green & Sustainable Science & Technology

Genome-Wide In Silico Identification, Structural Analysis, Promoter Analysis, and Expression Profiling of PHT Gene Family in Sugarcane Root under Salinity Stress

Naveenarani Murugan et al.

Summary: In this study, 23 PHT genes were identified in the sugarcane genome and their phylogeny, gene structure, and conserved motifs were analyzed. The results showed that these genes were mainly involved in phosphate ion transport and stress response, with PHT1-1, PHT1-2, and PHT1-3 members showing significant up-regulation under salt stress. Furthermore, the upstream region of PHT genes contained binding sites related to salt and phosphate stress. These findings provide insights into the evolution of sugarcane PHT genes and their role in salt and phosphate stress tolerance.

SUSTAINABILITY (2022)

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Review Cell Biology

CRISPR/Cas Genome Editing Technologies for Plant Improvement against Biotic and Abiotic Stresses: Advances, Limitations, and Future Perspectives

Yaxin Wang et al.

Summary: CRISPR/Cas-mediated genome editing is a game-changing tool for rapidly improving desirable traits in crops. It allows for easy applications, high efficiency, and low cost in producing targeted edits, leading to increased quality and yield in a shorter time. This technology holds great potential for creating crop varieties with resistance to biotic and abiotic stresses.

CELLS (2022)

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Review Genetics & Heredity

Recent Advances in Sugarcane Genomics, Physiology, and Phenomics for Superior Agronomic Traits

Mintu Ram Meena et al.

Summary: Advances in sugarcane breeding have contributed to improved agronomic traits and crop yield. However, further improvements are needed to meet the growing global demand for sugar and biofuel. The complex genetics of sugarcane and its heterozygous genome present challenges, but mapping techniques and genomics selection have allowed researchers to identify desirable alleles for superior agronomic traits.

FRONTIERS IN GENETICS (2022)

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Article Genetics & Heredity

CRISPR/Cas9-Mediated Targeting of Susceptibility Factor eIF4E-Enhanced Resistance Against Potato Virus Y

Azka Noureen et al.

Summary: This study demonstrates that using CRISPR/Cas9 technology to mutate the conserved region of the translation initiation factor eIF4E in potato can enhance resistance to Potato Virus Y (PVY). The mutated eIF4E protein is unable to interact with the virus, preventing its replication and systemic spread in the host. This provides a potential strategy to develop novel resistant potato cultivars against PVY.

FRONTIERS IN GENETICS (2022)

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Review Biochemistry & Molecular Biology

Virus-Induced Gene Editing and Its Applications in Plants

Chao Zhang et al.

Summary: CRISPR/Cas-based genome editing technologies have revolutionized plant science by allowing precise manipulation of plant genomes. Virus-induced genome editing (VIGE) has emerged as a powerful method for delivering CRISPR/Cas reagents into plant cells, with high editing efficiency and simplified process. This article provides an overview of CRISPR/Cas-based genome editing and focuses on the VIGE systems, types of viruses used for CRISPR/Cas9 cassette delivery, recent applications, and challenges and potential of VIGE in plants.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2022)

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Article Plant Sciences

CRISPR/Cas9 mediated gene-editing of GmHdz4 transcription factor enhances drought tolerance in soybean (Glycine max [L.] Merr.)

Xuanbo Zhong et al.

Summary: This study reported the characterization and function of GmHdz4, a HD-Zip I gene, in soybean response to drought stress. GmHdz4 was found to localize in the plant nucleus and display transcriptional activation activity, and its expression was induced by PEG-simulated drought stress. Editing of GmHdz4 using the CRISPR/Cas9 system was shown to enhance drought tolerance in soybean. These findings provide new insights for future molecular breeding research in soybean.

FRONTIERS IN PLANT SCIENCE (2022)

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Review Green & Sustainable Science & Technology

An Updated Overview on Insights into Sugarcane Genome Editing via CRISPR/Cas9 for Sustainable Production

Shareif Hammad Hussin et al.

Summary: This article synthesizes the fundamentals of genome editing techniques and explores the opportunities and challenges of sugarcane genome editing. The use of CRISPR/Cas9 system has successfully developed new sugarcane varieties with desired traits. Additionally, CRISPR/Cas9 gene editing holds enormous potential for improving sugarcane production and metabolic engineering.

SUSTAINABILITY (2022)

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Article Agronomy

Multiplex CRISPR/Cas9-mediated genome editing to address drought tolerance in wheat

Naglaa A. Abdallah et al.

Summary: Genome editing using a multiplex sgRNA-CRISPR/Cas9 system has been utilized to generate wheat mutants with TaSal1 gene mutations, resulting in phenotypic changes such as rolled leaves and bent stems. Anatomical and microscopic analysis of the mutated plants showed alterations in stomata and bulliform cells. This study demonstrates the efficiency of multiplex sgRNA-CRISPR/Cas9 genome editing in inducing mutations in hexaploid wheat.

GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN (2022)

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Article Plant Sciences

CRISPR-Cas9 Gene Editing of the Sal1 Gene Family in Wheat

Toni Mohr et al.

Summary: The highly conserved Sal1 gene has been edited in hexaploid wheat using precise gene editing techniques. The resulting mutant wheat plants with disabled Sal1 genes exhibit slower growth under drought conditions and slower senescence under water limiting conditions. The use of multiplexed gRNAs enabled effective targeted gene editing of the Sal1 gene family.

PLANTS-BASEL (2022)

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Review Biotechnology & Applied Microbiology

CRISPR/Cas genome editing improves abiotic and biotic stress tolerance of crops

Yangyang Li et al.

Summary: Abiotic and biotic stress factors limit plant growth and agricultural productivity, but genome editing techniques have great potential in breeding resistant varieties. The CRISPR/Cas technology has achieved remarkable results in resistance breeding of different crops, providing an important guarantee for stable crop yield worldwide.

FRONTIERS IN GENOME EDITING (2022)

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Article Plant Sciences

Advances in gene editing without residual transgenes in plants

Yubing He et al.

Summary: This article introduces several strategies to eliminate transgene residuals in edited plants, including direct editing of plant genomes with DNA-free reagents, using fluorescent markers, pigments, and chemical treatments to distinguish transgenic plants from transgene-free plants, and triggering self-elimination of transgenic plants through suicide genes. Additionally, it discusses the methods of excising transgenes from plant genomes using site-specific recombination, transposition, or gene editing nucleases, as well as the combination of haploid induction and gene editing for editing recalcitrant plants.

PLANT PHYSIOLOGY (2022)

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Article Plant Sciences

DNA-free CRISPR-Cas9 gene editing of wild tetraploid tomato Solanum peruvianum using protoplast regeneration

Choun-Sea Lin et al.

Summary: A DNA-free CRISPR-Cas9 genome editing tool based on an optimized protoplast regeneration protocol of wild tomato creates stable and inheritable diploid and tetraploid regenerants, which has significant implications for plant breeding and genetic research.

PLANT PHYSIOLOGY (2022)

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Article Plant Sciences

Inactivating transcription factor OsWRKY5 enhances drought tolerance through abscisic acid signaling pathways

Chaemyeong Lim et al.

Summary: In this study, the negative regulation of drought tolerance by the rice WRKY transcription factor OsWRKY5 was demonstrated. OsWRKY5 was mainly expressed in developing leaves at the seedling and heading stages, and its expression was reduced under drought stress and treatment with NaCl, mannitol, and ABA. Loss-of-function alleles of OsWRKY5 showed enhanced drought tolerance, while overexpression led to higher susceptibility. OsWRKY5 repression increased sensitivity to ABA and downregulated genes downstream of OsMYB2 in the ABA signaling pathways, indicating its role in negative regulation of ABA-induced drought stress tolerance.

PLANT PHYSIOLOGY (2022)

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Review Biotechnology & Applied Microbiology

Advances in Delivery Mechanisms of CRISPR Gene-Editing Reagents in Plants

Larissa C. Laforest et al.

Summary: CRISPR/Cas system revolutionized plant biology, significantly advancing plant breeding and improvement, but efficient delivery of editing reagents into plant cells remains a major challenge.

FRONTIERS IN GENOME EDITING (2022)

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Article Biotechnology & Applied Microbiology

CRISPR/Cas9 genome editing in ergot fungus Claviceps purpurea

Michaela Kralova et al.

Summary: This study demonstrated that CRISPR/Cas9 can be used for directed mutagenesis in Claviceps purpurea to generate homokaryotic mutants with uridine or tryptophan auxotrophy. However, the transformation efficiencies of CRISPR/Cas9 were only slightly improved compared to homologous recombination. Additionally, due to tryptophan deficiency, the fungi were non-infectious towards rye plants.

JOURNAL OF BIOTECHNOLOGY (2021)

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Article Plant Sciences

CRISPR/Cas9-based precise excision of SlHyPRP1 domain(s) to obtain salt stress-tolerant tomato

Mil Thi Tran et al.

Summary: CRISPR/Cas9 technology was used to precisely edit the protein domains of tomato hybrid proline-rich protein 1 (HyPRP1), resulting in increased salt tolerance. This gene editing approach may be an efficient tool for engineering multidomain proteins of important food crops to adapt to global climate changes for sustainable agriculture and future food security.

PLANT CELL REPORTS (2021)

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Review Biotechnology & Applied Microbiology

DNA-free gene editing in plants: a brief overview

Tsveta Tsanova et al.

Summary: The conversion of the bacterial CRISPR/Cas defense system into a tool for genome manipulations revolutionized experimental biology by providing a simple and efficient method for genome editing. CRISPR/Cas gene editing became the second most important technology in plant biology and biotechnology, relying on various methods of gene delivery. Novel methods such as in vitro ribonucleoprotein complexes formulations and the use of virus-like particles have been developed to achieve DNA-free editing, opening up new possibilities for advancements in the field.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT (2021)

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Article Biochemistry & Molecular Biology

First Report of CRISPR/Cas9 Mediated DNA-Free Editing of 4CL and RVE7 Genes in Chickpea Protoplasts

Sapna Badhan et al.

Summary: The current genome editing system CRISPR/Cas9 has been proven effective in plant applications, with the DNA-free editing showing promise in achieving targeted mutagenesis in chickpea. By selecting drought tolerance-related genes 4CL and RVE7 for editing in chickpea protoplast, this study demonstrated high efficiency in editing the RVE7 gene, providing insights into gene functions under drought stress in chickpea.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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Article Plant Sciences

Efficient Cas9 multiplex editing using unspaced sgRNA arrays engineering in a Potato virus X vector

Mireia Uranga et al.

Summary: The development of a PVX-based vector allows for the easy and efficient expression of multiple sgRNAs for multiplex CRISPR-Cas genome editing in adult Solanaceae plants, achieving nearly 80% indels in targeted genes within a few days. The vector also enables the production of virus-free edited progeny with high rates of heritable biallelic mutations, making it a valuable tool for functional gene analysis and precision breeding in diverse plant species, particularly Solanaceae crops.

PLANT JOURNAL (2021)

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Article Biochemical Research Methods

Overexpression of SoACLA-1 Gene Confers Drought Tolerance Improvement in Sugarcane

Kai Zhu et al.

Summary: This study cloned and transformed the sugarcane ACL gene SoACLA-1, finding that overexpression of SoACLA-1 gene can enhance drought tolerance in transgenic sugarcane plants. Through physiological and biochemical analysis, as well as gene expression studies, the results showed that transgenic sugarcane plants exhibited better resistance to drought and recovery ability.

PLANT MOLECULAR BIOLOGY REPORTER (2021)

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Review Biochemistry & Molecular Biology

An Outlook on Global Regulatory Landscape for Genome-Edited Crops

Aftab Ahmad et al.

Summary: The revolutionary CRISPR/Cas technology has led to its developers winning the 2020 Nobel Prize for Chemistry. In agriculture, CRISPR systems have accelerated the development of new crop varieties without the need for transgenes. However, the future of this technology depends on the establishment of a clear global regulatory framework, as there is still ongoing debate and division over the legal status of CRISPR-edited crops.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

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Article Biochemical Research Methods

Evaluation of CRISPR/Cas9 site-specific function and validation of sgRNA sequence by a Cas9/sgRNA-assisted reverse PCR technique

Beibei Zhang et al.

Summary: The study developed a CRISPR/Cas9-assisted reverse PCR method to address off-target effects and loci-affinity issues of Cas9-sgRNA. This method enables rapid site-specific detection and sgRNA sequence validation in the laboratory, serving as an effective tool for CRISPR/Cas9 system research.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2021)

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Article Biochemistry & Molecular Biology

Establishment of an efficient seed fluorescence reporter-assisted CRISPR/Cas9 gene editing in maize

Yuanyuan Yan et al.

Summary: The CRISPR/Cas9 genome editing technique has been established in maize using a seed fluorescence reporter system, allowing for the successful generation of desired mutants or mutant libraries. This approach may be particularly valuable for scenarios with low transformation frequency and has the potential to be expanded to other important monocot seed crops.

JOURNAL OF INTEGRATIVE PLANT BIOLOGY (2021)

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Article Biotechnology & Applied Microbiology

A piggyBac-mediated transgenesis system for the temporary expression of CRISPR/Cas9 in rice

Ayako Nishizawa-Yokoi et al.

Summary: The study presents a piggyBac-mediated transgenesis system for temporary expression of CRISPR/Cas9 in plants, enabling precise excision of the piggyBac after successful targeted mutagenesis induction. The system demonstrated successful transgenesis in rice plants and the detection of CRISPR/Cas9-induced mutations even after piggyBac excision, suggesting its potential as an efficient tool for CRISPR/Cas9-mediated targeted mutagenesis in vegetatively propagated crops.

PLANT BIOTECHNOLOGY JOURNAL (2021)

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Article Biochemical Research Methods

Heritable gene editing using FT mobile guide RNAs and DNA viruses

Jianfeng Lei et al.

Summary: The virus-induced genome editing (VIGE) system has great application potential, but it cannot transfer edited genes to the next generation due to the inability of viruses to enter shoot apical meristem (SAM) in plants. A novel CLCrV-mediated VIGE system using the FT strategy enables heritable mutant offspring without tissue culture in A. thaliana.

PLANT METHODS (2021)

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Article Virology

Genome Editing of Rice eIF4G Loci Confers Partial Resistance to Rice Black-Streaked Dwarf Virus

Wei Wang et al.

Summary: This study successfully generated eIF4G mutants with partial resistance to rice black-streaked dwarf virus (RBSDV) through CRISPR/Cas9 technology, which exhibited enhanced tolerance to virus infection. However, the mutants showed the same sensitivity to rice stripe virus (RSV) infection as wild-type plants, indicating specificity in resistance mechanisms against different viruses. Additionally, the eIF4G mutants had reduced eIF4G transcript levels and interacted directly with RBSDV, highlighting their potential as disease-resistant resources for developing RBSDV-resistant varieties.

VIRUSES-BASEL (2021)

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Article Plant Sciences

Mutation of GmAITR Genes by CRISPR/Cas9 Genome Editing Results in Enhanced Salinity Stress Tolerance in Soybean

Tianya Wang et al.

Summary: This study demonstrates that mutation of GmAITR genes using CRISPR/Cas9 can enhance salinity tolerance in soybean, improving stress resistance at the molecular level. Targeting multiple GmAITR genes simultaneously successfully generated Cas9-free mutants, which showed increased ABA sensitivity and enhanced salt tolerance. These results highlight the efficiency of CRISPR/Cas9 in improving salinity tolerance in soybean.

FRONTIERS IN PLANT SCIENCE (2021)

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Article Plant Sciences

A Sugarcane G-Protein-Coupled Receptor, ShGPCR1, Confers Tolerance to Multiple Abiotic Stresses

Manikandan Ramasamy et al.

Summary: The study identified a GPCR-like protein (ShGPCR1) in sugarcane that plays a key role in conferring tolerance to multiple abiotic stresses such as drought, salinity, and cold. Overexpression of ShGPCR1 in sugarcane enhanced stress tolerance and activated stress marker genes, suggesting its potential for improving sugarcane production in challenging environments.

FRONTIERS IN PLANT SCIENCE (2021)

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Review Plant Sciences

Genetic Transformation of Sugarcane, Current Status and Future Prospects

Florencia Budeguer et al.

Summary: Sugarcane is a crucial tropical crop with complex genetics and difficulty in traditional breeding improvement due to its large genome and low fertility. Genetic engineering offers a promising approach for sugarcane improvement, but technical challenges remain to be addressed.

FRONTIERS IN PLANT SCIENCE (2021)

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Article Plant Sciences

Highly efficient generation of bacterial leaf blight-resistant and transgene-free rice using a genome editing and multiplexed selection system

Kun Yu et al.

Summary: Core nucleotide deletion in the UPT box of the Xa13 promoter conferred resistance to rice blight, and the selection of transgene-free plants was boosted by introducing multiplexed selection. The combination of genome editing and transgene-free selection is an efficient strategy to accelerate functional genomic research and plant breeding.

BMC PLANT BIOLOGY (2021)

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Article Genetics & Heredity

CRISPR-Cas12a Genome Editing at the Whole-Plant Level Using Two Compatible RNA Virus Vectors

Mireia Uranga et al.

Summary: The successful DNA-free genome editing of Nicotiana benthamiana using two compatible RNA virus vectors derived from tobacco etch virus (TEV) and potato virus X (PVX) represents a novel approach to plant genome editing. This two-virus vector system, which respectively express a Cas12a nuclease and the corresponding guide RNA, enhances the toolbox for transformation-free virus-induced genome editing in plants, with the potential to advance efforts in breeding more nutritious, resistant, and productive crops.

CRISPR JOURNAL (2021)

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Letter Biotechnology & Applied Microbiology

CRISPR/Cas9-mediated editing of DMR6 orthologue in banana (Musa spp.) confers enhanced resistance to bacterial disease

Jaindra N. Tripathi et al.

PLANT BIOTECHNOLOGY JOURNAL (2021)

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Article Multidisciplinary Sciences

RNA silencing suppressor-influenced performance of a virus vector delivering both guide RNA and Cas9 for CRISPR gene editing

Kelvin T. Chiong et al.

Summary: This study demonstrates that using viral RNA suppressors can enhance the efficiency of CRISPR/Cas9 gene editing by regulating the expression of Cas9. Age was found to affect the efficacy of the RNA suppressor P19 during gene editing in plants. Co-delivery of Cas9 and sgRNA using a single viral vector can improve editing efficiency.

SCIENTIFIC REPORTS (2021)

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Review Plant Sciences

Sugarcane Smut, Caused by Sporisorium scitamineum, a Major Disease of Sugarcane: A Contemporary Review

Shamsul A. Bhuiyan et al.

Summary: Sugarcane smut caused by the fungus Sporisorium scitamineum is a major disease worldwide, primarily spread through airborne teliospores. Resistant varieties are the most effective management strategy, but difficulties in selecting for resistant traits due to the complex genomic makeup of sugarcane have led to investigations into using molecular markers for early selection. Advances in omics technologies have provided insights into resistance mechanisms and may contribute to developing durable resistant varieties.

PHYTOPATHOLOGY (2021)

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Article Multidisciplinary Sciences

Loss of function of a DMR6 ortholog in tomato confers broad-spectrum disease resistance

Daniela Paula de Toledo Thomazella et al.

Summary: The study reveals that disabling susceptibility (S) genes is a promising alternative to confer durable and broad-spectrum disease resistance in breeding programs. In tomato, SlDMR6-1 shows enhanced resistance to various pathogens when up-regulated, accompanied by increased salicylic acid (SA) levels and transcriptional activation of immune responses.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2021)

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Review Microbiology

Sugarcane Mosaic Disease: Characteristics, Identification and Control

Guilong Lu et al.

Summary: Mosaic is a significant sugarcane disease caused by single or compound infection of three viruses, leading to decreased yield and economic losses. Understanding the epidemiology, genetic diversity, identification methods, and prevention measures is crucial for effective management of mosaic disease in sugarcane through resistance breeding.

MICROORGANISMS (2021)

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Article

Expression analysis of transcription factors in sugarcane during cold stress

S. U. Rehman et al.

BRAZILIAN JOURNAL OF BIOLOGY (2021)

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Article Biotechnology & Applied Microbiology

CRISPR/Cas9-Mediated Multi-Allelic Gene Targeting in Sugarcane Confers Herbicide Tolerance

Mehmet Tufan Oz et al.

Summary: The study successfully achieved efficient and reproducible gene targeting in sugarcane, allowing precise co-editing of multiple alleles to improve crop tolerance and yield.

FRONTIERS IN GENOME EDITING (2021)

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Review Biochemistry & Molecular Biology

Genetically modified crop regulations: scope and opportunity using the CRISPR-Cas9 genome editing approach

Shweta Gupta et al.

Summary: The global demand for food is increasing due to population growth and land scarcity, prompting the development of high-yielding and genetically modified crops. Advances in gene-editing technologies have introduced new techniques for precise gene editing without introducing foreign genes, but the regulation and public acceptance of these technologies remain a topic of debate. Different countries have varying approaches to regulating gene-edited plants, which can impact their commercial value and adoption by farmers. Strong and clear regulatory frameworks are needed to support the adoption of emerging crop technologies.

MOLECULAR BIOLOGY REPORTS (2021)

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Article Biotechnology & Applied Microbiology

Multiallelic, Targeted Mutagenesis of Magnesium Chelatase With CRISPR/Cas9 Provides a Rapidly Scorable Phenotype in Highly Polyploid Sugarcane

Ayman Eid et al.

Summary: Genome editing with CRISPR/Cas9 technology in sugarcane resulted in efficient editing of the magnesium chelatase gene, leading to significantly reduced chlorophyll content. Heat treatment and co-editing of multiple alleles promoted editing frequency and the creation of a distinguishable phenotype from the wild type.

FRONTIERS IN GENOME EDITING (2021)

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Article Agronomy

Optimization of protoplast isolation, transformation and its application in sugarcane (Saccharum spontaneum L)

Qiongli Wang et al.

Summary: The study optimized protoplast isolation and PEG-mediated transformation conditions in S. spontaneum, establishing an efficient transformation system for gene analysis and promoter activity studies in sugarcane.

CROP JOURNAL (2021)

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Article Plant Sciences

Engineering abiotic stress tolerance via CRISPR/Cas-mediated genome editing

Syed Adeel Zafar et al.

JOURNAL OF EXPERIMENTAL BOTANY (2020)

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Article Plant Sciences

Lipofection-mediated genome editing using DNA-free delivery of the Cas9/gRNA ribonucleoprotein into plant cells

Wusheng Liu et al.

PLANT CELL REPORTS (2020)

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Article Genetics & Heredity

An RNAi suppressor activatesin plantavirus-mediated gene editing

Xian Zhang et al.

FUNCTIONAL & INTEGRATIVE GENOMICS (2020)

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Article Biotechnology & Applied Microbiology

Multiplex CRISPR/Cas9-mediated metabolic engineering increases soya bean isoflavone content and resistance to soya bean mosaic virus

Peipei Zhang et al.

PLANT BIOTECHNOLOGY JOURNAL (2020)

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Article Agronomy

Comparison of CRISPR-Cas9/Cas12a Ribonucleoprotein Complexes for Genome Editing Efficiency in the Rice Phytoene Desaturase (OsPDS) Gene

Raviraj Banakar et al.

RICE (2020)

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Article Biotechnology & Applied Microbiology

Generation of transgene-free PDS mutants in potato by Agrobacterium-mediated transformation

Zsofia Banfalvi et al.

BMC BIOTECHNOLOGY (2020)

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Article Genetics & Heredity

Down Regulation and Loss of Auxin Response Factor 4 Function Using CRISPR/Cas9 Alters Plant Growth, Stomatal Function and Improves Tomato Tolerance to Salinity and Osmotic Stress

Sarah Bouzroud et al.

GENES (2020)

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Article Plant Sciences

Mutations in the tomato gibberellin receptors suppress xylem proliferation and reduce water loss under water-deficit conditions

Natanella Illouz-Eliaz et al.

JOURNAL OF EXPERIMENTAL BOTANY (2020)

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Article Biotechnology & Applied Microbiology

High-throughput sequencing reveals genes associated with high-temperature stress tolerance in sugarcane

Gomathi Raju et al.

3 BIOTECH (2020)

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Article Plant Sciences

CRISPR-Cas9 mediated genome editing of drought and salt tolerance (OsDST) gene in indica mega rice cultivar MTU1010

V. V. Santosh Kumar et al.

PHYSIOLOGY AND MOLECULAR BIOLOGY OF PLANTS (2020)

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Article Biochemistry & Molecular Biology

Virus-induced CRISPR-Cas9 system improved resistance against tomato yellow leaf curl virus

Parisa Ghorbani Faal et al.

MOLECULAR BIOLOGY REPORTS (2020)

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Article Plant Sciences

CRISPR/Cas9-targeted mutagenesis of the tomato susceptibility gene PMR4 for resistance against powdery mildew

Miguel I. Santillan Martinez et al.

BMC PLANT BIOLOGY (2020)

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Article Plant Sciences

CRISPR/Cas9-mediated mutagenesis of VvMLO3 results in enhanced resistance to powdery mildew in grapevine (Vitis vinifera)

Dong-Yan Wan et al.

HORTICULTURE RESEARCH (2020)

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Article Plant Sciences

Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

Shaoping Wu et al.

BMC PLANT BIOLOGY (2020)

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Article Biotechnology & Applied Microbiology

A rapid method for detection of mutations induced by CRISPR/Cas9-based genome editing in common wheat

Yoko Kamiya et al.

PLANT BIOTECHNOLOGY (2020)

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Article Plant Sciences

OsNRAMP1transporter contributes to cadmium and manganese uptake in rice

Jia-Dong Chang et al.

PLANT CELL AND ENVIRONMENT (2020)

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Article Biochemistry & Molecular Biology

Precise Editing of the OsPYL9 Gene by RNA-Guided Cas9 Nuclease Confers Enhanced Drought Tolerance and Grain Yield in Rice (Oryza sativa L.) by Regulating Circadian Rhythm and Abiotic Stress Responsive Proteins

Babar Usman et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2020)

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Article Plant Sciences

Potato Virus X Vector-Mediated DNA-Free Genome Editing in Plants

Hirotaka Ariga et al.

PLANT AND CELL PHYSIOLOGY (2020)

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Review Biotechnology & Applied Microbiology

Current breeding and genomic approaches to enhance the cane and sugar productivity under abiotic stress conditions

Mintu Ram Meena et al.

3 BIOTECH (2020)

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Review Genetics & Heredity

Structural Aspects of DNA Repair and Recombination in Crop Improvement

Prabha Verma et al.

FRONTIERS IN GENETICS (2020)

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Article Plant Sciences

OsmiR535, a Potential Genetic Editing Target for Drought and Salinity Stress Tolerance in Oryza sativa

Erkui Yue et al.

PLANTS-BASEL (2020)

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Article Multidisciplinary Sciences

CRISPR/Cas9-targeted mutagenesis of OsERA1 confers enhanced responses to abscisic acid and drought stress and increased primary root growth under nonstressed conditions in rice

Takuya Ogata et al.

PLOS ONE (2020)

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Article Plant Sciences

Accurate Detection and Evaluation of the Gene-Editing Frequency in Plants Using Droplet Digital PCR

Cheng Peng et al.

FRONTIERS IN PLANT SCIENCE (2020)

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Article Biotechnology & Applied Microbiology

Repurposing of Anthocyanin Biosynthesis for Plant Transformation and Genome Editing

Yubing He et al.

FRONTIERS IN GENOME EDITING (2020)

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Article Plant Sciences

Highly efficient DNA-free plant genome editing using virally delivered CRISPR-Cas9

Xiaonan Ma et al.

NATURE PLANTS (2020)

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Article Plant Sciences

Multiplexed heritable gene editing using RNA viruses and mobile single guide RNAs

Evan E. Ellison et al.

NATURE PLANTS (2020)

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Article Multidisciplinary Sciences

CRISPR-CasΦ from huge phages is a hypercompact genome editor

Patrick Pausch et al.

SCIENCE (2020)

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Article Biotechnology & Applied Microbiology

Design of a bacterial speck resistant tomato by CRISPR/Cas9-mediated editing of SlJAZ2

Andres Ortigosa et al.

PLANT BIOTECHNOLOGY JOURNAL (2019)

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Article Multidisciplinary Sciences

CasX enzymes comprise a distinct family of RNA-guided genome editors

Jun-Jie Liu et al.

NATURE (2019)

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Article Agronomy

Improvements of TKC Technology Accelerate Isolation of Transgene-Free CRISPR/Cas9-Edited Rice Plants

He Yubing et al.

RICE SCIENCE (2019)

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Article Plant Sciences

Overexpression of an evolutionarily conserved drought-responsive sugarcane gene enhances salinity and drought resilience

Kevin Begcy et al.

ANNALS OF BOTANY (2019)

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Article Plant Sciences

Rescue of a plant cytorhabdovirus as versatile expression platforms for planthopper and cereal genomic studies

Qiang Gao et al.

NEW PHYTOLOGIST (2019)

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Article Biochemistry & Molecular Biology

CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

Kornel Labun et al.

NUCLEIC ACIDS RESEARCH (2019)

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Letter Biotechnology & Applied Microbiology

Cas9-PF, an early flowering and visual selection marker system, enhances the frequency of editing event occurrence and expedites the isolation of genome-edited and transgene-free plants

Yong Liu et al.

PLANT BIOTECHNOLOGY JOURNAL (2019)

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Article Plant Sciences

A barley stripe mosaic virus-based guide RNA delivery system for targeted mutagenesis in wheat and maize

Jiacheng Hu et al.

MOLECULAR PLANT PATHOLOGY (2019)

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Article Plant Sciences

Identification of Transgene-Free CRISPR-Edited Plants of Rice, Tomato, and Arabidopsis by Monitoring DsRED Fluorescence in Dry Seeds

Norma Aliaga-Franco et al.

FRONTIERS IN PLANT SCIENCE (2019)

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Article Biochemical Research Methods

Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing

Yingchun Zhang et al.

BIOTECHNIQUES (2019)

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Article Biochemistry & Molecular Biology

Transgene-Free Genome Editing in Tomato and Potato Plants Using Agrobacterium-Mediated Delivery of a CRISPR/Cas9 Cytidine Base Editor

Florian Veillet et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2019)

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Article Biotechnology & Applied Microbiology

Development of Beet necrotic yellow vein virus-based vectors for multiple-gene expression and guide RNA delivery in plant genome editing

Ning Jiang et al.

PLANT BIOTECHNOLOGY JOURNAL (2019)

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Article Plant Sciences

Efficient and transgene-free gene targeting using Agrobacterium-mediated delivery of the CRISPR/Cas9 system in tomato

Benoit Danilo et al.

PLANT CELL REPORTS (2019)

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Review Plant Sciences

DNA-Free Genome Editing: Past, Present and Future

Janina Metje-Sprink et al.

FRONTIERS IN PLANT SCIENCE (2019)

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Article Multidisciplinary Sciences

High-frequency random DNA insertions upon co-delivery of CRISPR-Cas9 ribonucleoprotein and selectable marker plasmid in rice

Raviraj Banakar et al.

SCIENTIFIC REPORTS (2019)

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Article Genetics & Heredity

Evolving Regulatory Landscape for Genome-Edited Plants

Lisa Zannoni

CRISPR JOURNAL (2019)

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Article Plant Sciences

Protein expression and gene editing in monocots using foxtail mosaic virus vectors

Yu Mei et al.

PLANT DIRECT (2019)

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Article Plant Sciences

An efficient DNA- and selectable-marker-free genome-editing system using zygotes in rice

Erika Toda et al.

NATURE PLANTS (2019)

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Article Biology

CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

Jaindra N. Tripathi et al.

COMMUNICATIONS BIOLOGY (2019)

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Article Genetics & Heredity

CRISPR/Cas9 genome editing in wheat

Dongjin Kim et al.

FUNCTIONAL & INTEGRATIVE GENOMICS (2018)

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Review Biochemistry & Molecular Biology

Nonhomologous DNA end-joining for repair of DNA double-strand breaks

Nicholas R. Pannunzio et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2018)

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Article Plant Sciences

Disruption of OsSEC3A increases the content of salicylic acid and induces plant defense responses in rice

Jin Ma et al.

JOURNAL OF EXPERIMENTAL BOTANY (2018)

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Article Agronomy

Isolation, Transformation and Overexpression of Sugarcane SoP5CS Gene for Drought Tolerance Improvement

Jian Li et al.

SUGAR TECH (2018)

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Article Agronomy

Molecular detection and prevalence of Xanthomonas albilineans, the causal agent of sugarcane leaf scald, in China

Ling-Hong Lin et al.

CROP PROTECTION (2018)

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Article Biochemistry & Molecular Biology

Programmed Self-Elimination of the CRISPR/Cas9 Construct Greatly Accelerates the Isolation of Edited and Transgene-Free Rice Plants

Yubing He et al.

MOLECULAR PLANT (2018)

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Article Biochemical Research Methods

Genome editing of bread wheat using biolistic delivery of CRISPR/Cas9 &ITin vitro&IT transcripts or ribonucleoproteins

Zhen Liang et al.

NATURE PROTOCOLS (2018)

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Article Plant Sciences

Genome editing in potato via CRISPR-Cas9 ribonucleoprotein delivery

Mariette Andersson et al.

PHYSIOLOGIA PLANTARUM (2018)

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Article Biotechnology & Applied Microbiology

Novel alleles of rice eIF4G generated by CRISPR/Cas9-targeted mutagenesis confer resistance to Rice tungro spherical virus

Anca Macovei et al.

PLANT BIOTECHNOLOGY JOURNAL (2018)

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Article Plant Sciences

Efficient in planta gene targeting in tomato using geminiviral replicons and the CRISPR/Cas9 system

Tal Dahan-Meir et al.

PLANT JOURNAL (2018)

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Article Virology

Pea early-browning virus-mediated genome editing via the CRISPR/Cas9 system in Nicotiana benthamiana and Arabidopsis

Zahir Ali et al.

VIRUS RESEARCH (2018)

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Article Multidisciplinary Sciences

A mosaic monoploid reference sequence for the highly complex genome of sugarcane

Olivier Garsmeur et al.

NATURE COMMUNICATIONS (2018)

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Article Multidisciplinary Sciences

A Survey of Validation Strategies for CRISPR-Cas9 Editing

Monica F. Sentmanat et al.

SCIENTIFIC REPORTS (2018)

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Review Plant Sciences

Heavy Metal Stress, Signaling, and Tolerance Due to Plant-Associated Microbes: An Overview

Shalini Tiwari et al.

FRONTIERS IN PLANT SCIENCE (2018)

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Article Plant Sciences

A Novel L-ascorbate Peroxidase 6 Gene, ScAPX6, Plays an Important Role in the Regulation of Response to Biotic and Abiotic Stresses in Sugarcane

Feng Liu et al.

FRONTIERS IN PLANT SCIENCE (2018)

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Article Plant Sciences

Transient Expression of CRISPR/Cas9 Machinery Targeting TcNPR3 Enhances Defense Response in Theobroma cacao

Andrew S. Fister et al.

FRONTIERS IN PLANT SCIENCE (2018)

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Article Plant Sciences

A method for the production and expedient screening of CRISPR/Cas9-mediated non-transgenic mutant plants

Longzheng Chen et al.

HORTICULTURE RESEARCH (2018)

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Article Biochemistry & Molecular Biology

CRISPR/Cas9-Mediated Multiplex Genome Editing of the BnWRKY11 and BnWRKY70 Genes in Brassica napus L.

Qinfu Sun et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2018)

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Article Entomology

Biology, natural enemies and damage of the sugarcane whitefly (Neomaskellia andropogonis) (Homoptera: Aleyrodidae) in Iran

Alireza Askarianzadeh et al.

INTERNATIONAL JOURNAL OF TROPICAL INSECT SCIENCE (2018)

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Article Genetics & Heredity

Allele-defined genome of the autopolyploid sugarcane Saccharum spontaneum L.

Jisen Zhang et al.

NATURE GENETICS (2018)

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Article Plant Sciences

DNA-Free Genome Editing of Brassica oleracea and B. rapa Protoplasts Using CRISPR-Cas9 Ribonucleoprotein Complexes

Jana Murovec et al.

FRONTIERS IN PLANT SCIENCE (2018)

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Article Environmental Sciences

Alleviation of heavy metals toxicity by the application of plant growth promoting rhizobacteria and effects on wheat grown in saline sodic field

Tamoor Ul Hassan et al.

INTERNATIONAL JOURNAL OF PHYTOREMEDIATION (2017)

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Article Agriculture, Multidisciplinary

Reduced Drought Tolerance by CRISPR/Cas9-Mediated SlMAPK3 Mutagenesis in Tomato Plants

Liu Wang et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2017)

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Letter Biochemistry & Molecular Biology

CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants

Hao Liu et al.

MOLECULAR PLANT (2017)

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Letter Biochemistry & Molecular Biology

Gene Targeting by Homology-Directed Repair in Rice Using a Geminivirus-Based CRISPR/Cas9 System

Mugui Wang et al.

MOLECULAR PLANT (2017)

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Article Biotechnology & Applied Microbiology

Precise base editing in rice, wheat and maize with a Cas9-cytidine deaminase fusion

Yuan Zong et al.

NATURE BIOTECHNOLOGY (2017)

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Review Biotechnology & Applied Microbiology

New traits in crops produced by genome editing techniques based on deletions

C. C. M. van de Wiel et al.

PLANT BIOTECHNOLOGY REPORTS (2017)

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Article Plant Sciences

High-efficiency gene targeting in hexaploid wheat using DNA replicons and CRISPR/Cas9

Javier Gil-Humanes et al.

PLANT JOURNAL (2017)

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Article Plant Sciences

Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector

Will B. Cody et al.

PLANT PHYSIOLOGY (2017)

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Review Plant Sciences

Sugarcane Water Stress Tolerance Mechanisms and Its Implications on Developing Biotechnology Solutions

Thais H. S. Ferreira et al.

FRONTIERS IN PLANT SCIENCE (2017)

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Article Plant Sciences

OsARM1, an R2R3 MYB Transcription Factor, Is Involved in Regulation of the Response to Arsenic Stress in Rice

Feng-Zhu Wang et al.

FRONTIERS IN PLANT SCIENCE (2017)

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Article Biotechnology & Applied Microbiology

Red rot resistant gene characterization using RGAP markers among sugarcane cultivars resistant and susceptible to the red rot disease

Ruchika Sharma et al.

3 BIOTECH (2017)

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Article Biotechnology & Applied Microbiology

Red rot resistant gene characterization using RGAP markers among sugarcane cultivars resistant and susceptible to the red rot disease

Ruchika Sharma et al.

3 BIOTECH (2017)

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Article Multidisciplinary Sciences

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion

Vladimir Nekrasov et al.

SCIENTIFIC REPORTS (2017)

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Article Multidisciplinary Sciences

Rapid breeding of parthenocarpic tomato plants using CRISPR/Cas9

Risa Ueta et al.

SCIENTIFIC REPORTS (2017)

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Review Cell Biology

Error-Prone Repair of DNA Double-Strand Breaks

Kasey Rodgers et al.

JOURNAL OF CELLULAR PHYSIOLOGY (2016)

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Article Biotechnology & Applied Microbiology

De novo analysis of transcriptome reveals genes associated with leaf abscission in sugarcane (Saccharum officinarum L.)

Ming Li et al.

BMC GENOMICS (2016)

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Article Geography, Physical

Agricultural drought monitoring: Progress, challenges, and prospects

Liu Xianfeng et al.

JOURNAL OF GEOGRAPHICAL SCIENCES (2016)

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Article Plant Sciences

Development of broad virus resistance in non-transgenic cucumber using CRISPR/Cas9 technology

Jeyabharathy Chandrasekaran et al.

MOLECULAR PLANT PATHOLOGY (2016)

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Article Plant Sciences

Regulatory hurdles for genome editing: process- vs. product-based approaches in different regulatory contexts

Thorben Sprink et al.

PLANT CELL REPORTS (2016)

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Article Plant Sciences

An Effective Strategy for Reliably Isolating Heritable and Cas9-Free Arabidopsis Mutants Generated by CRISPR/Cas9-Mediated Genome Editing

Xiuhua Gao et al.

PLANT PHYSIOLOGY (2016)

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Article Multidisciplinary Sciences

Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes

Sergei Svitashev et al.

NATURE COMMUNICATIONS (2016)

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Article Multidisciplinary Sciences

Enhanced Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922

Fujun Wang et al.

PLOS ONE (2016)

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Article Multidisciplinary Sciences

CRISPR/Cas9-mediated efficient and heritable targeted mutagenesis in tomato plants in the first and later generations

Changtian Pan et al.

SCIENTIFIC REPORTS (2016)

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Article Plant Sciences

SoMYB18, a sugarcane MYB transcription factor improves salt and dehydration tolerance in tobacco

Prashant Raghunath Shingote et al.

ACTA PHYSIOLOGIAE PLANTARUM (2015)

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Editorial Material Biochemistry & Molecular Biology

Plant grafting

Charles W. Melnyk et al.

CURRENT BIOLOGY (2015)

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Letter Biochemistry & Molecular Biology

A Simple CRISPR/Cas9 System for Multiplex Genome Editing in Rice

Chun Wang et al.

JOURNAL OF GENETICS AND GENOMICS (2015)

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Article Agronomy

Variability in yellow leaf symptom expression caused by the Sugarcane yellow leaf virus and its seasonal influence in sugarcane

C. Chinnaraja et al.

PHYTOPARASITICA (2015)

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Article Plant Sciences

Agrobacterium-mediated in planta genetic transformation of sugarcane setts

Subramanian Mayavan et al.

PLANT CELL REPORTS (2015)

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Article Multidisciplinary Sciences

Cold Responsive Gene Expression Profiling of Sugarcane and Saccharum spontaneum with Functional Analysis of a Cold Inducible Saccharum Homolog of NOD26-Like Intrinsic Protein to Salt and Water Stress

Jong-Won Park et al.

PLOS ONE (2015)

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Article Multidisciplinary Sciences

A geminivirus-based guide RNA delivery system for CRISPR/Cas9 mediated plant genome editing

Kangquan Yin et al.

SCIENTIFIC REPORTS (2015)

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Editorial Material Agronomy

Genome editing for crop improvement: Challenges and opportunities

Naglaa A. Abdallah et al.

GM CROPS & FOOD-BIOTECHNOLOGY IN AGRICULTURE AND THE FOOD CHAIN (2015)

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Article Multidisciplinary Sciences

Generation and Inheritance of Targeted Mutations in Potato (Solanum tuberosum L.) Using the CRISPR/Cas System

Nathaniel M. Butler et al.

PLOS ONE (2015)

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Review Agronomy

Adaptive Responses of Sugarcane to Waterlogging Stress: An Over View

R. Gomathi et al.

SUGAR TECH (2015)

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Article Biotechnology & Applied Microbiology

High-frequency, precise modification of the tomato genome

Tomas Cermak et al.

GENOME BIOLOGY (2015)

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Article Plant Sciences

Impact of Sugarcane yellow leaf virus (ScYLV) infection on physiological efficiency and growth parameters of sugarcane under tropical climatic conditions in India

R. Viswanathan et al.

ACTA PHYSIOLOGIAE PLANTARUM (2014)

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Article Biotechnology & Applied Microbiology

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

Yanpeng Wang et al.

NATURE BIOTECHNOLOGY (2014)

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Article Biochemistry & Molecular Biology

DNA Replicons for Plant Genome Engineering

Nicholas J. Baltes et al.

PLANT CELL (2014)

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Article Multidisciplinary Sciences

Efficient CRISPR/Cas9-Mediated Gene Editing in Arabidopsis thaliana and Inheritance of Modified Genes in the T2 and T3 Generations

WenZhi Jiang et al.

PLOS ONE (2014)

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Review Environmental Sciences

Reactive oxygen species (ROS) and response of antioxidants as ROS-scavengers during environmental stress in plants

Kaushik Das et al.

FRONTIERS IN ENVIRONMENTAL SCIENCE (2014)

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Article Plant Sciences

Agrobacterium tumefaciens-mediated in planta seed transformation strategy in sugarcane

Subramanian Mayavan et al.

PLANT CELL REPORTS (2013)

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Article Multidisciplinary Sciences

High-Throughput Sequencing of Small RNA Transcriptome Reveals Salt Stress Regulated MicroRNAs in Sugarcane

Mariana Carnavale Bottino et al.

PLOS ONE (2013)

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Review Biotechnology & Applied Microbiology

Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding

Nancy Podevin et al.

TRENDS IN BIOTECHNOLOGY (2013)

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Article Agronomy

Sugarcane for water-limited environments: Theoretical assessment of suitable traits

N. G. Inman-Bamber et al.

FIELD CROPS RESEARCH (2012)

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Review Biochemistry & Molecular Biology

Playing the End Game: DNA Double-Strand Break Repair Pathway Choice

J. Ross Chapman et al.

MOLECULAR CELL (2012)

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Article Plant Sciences

A novel dirigent protein gene with highly stem-specific expression from sugarcane, response to drought, salt and oxidative stresses

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Article Multidisciplinary Sciences

A Novel Stress-Induced Sugarcane Gene Confers Tolerance to Drought, Salt and Oxidative Stress in Transgenic Tobacco Plants

Kevin Begcy et al.

PLOS ONE (2012)

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Article Multidisciplinary Sciences

microRNAs Associated with Drought Response in the Bioenergy Crop Sugarcane (Saccharum spp.)

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PLOS ONE (2012)

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Article Multidisciplinary Sciences

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

Martin Jinek et al.

SCIENCE (2012)

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Article Plant Sciences

Developing stress tolerant plants through in vitro selection-An overview of the recent progress

Manoj K. Rai et al.

ENVIRONMENTAL AND EXPERIMENTAL BOTANY (2011)

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Review Agronomy

Sugarcane By-Products Based Industries in India

S. Solomon

SUGAR TECH (2011)

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Article Agronomy

Genetic Diversity of Sugarcane Grassy Shoot (SCGS)-Phytoplasmas Causing Grassy Shoot Disease in India

R. Viswanathan et al.

SUGAR TECH (2011)

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Review Agronomy

Disease Scenario and Management of Major Sugarcane Diseases in India

R. Viswanathan et al.

SUGAR TECH (2011)

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Article Plant Sciences

Sugarcane Under Pressure: An Overview of Biochemical and Physiological Studies of Abiotic Stress

Ricardo A. Azevedo et al.

Tropical Plant Biology (2011)

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Article Plant Sciences

Selection system and co-cultivation medium are important determinants of Agrobacterium-mediated transformation of sugarcane

Priya Joyce et al.

PLANT CELL REPORTS (2010)

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Article Environmental Sciences

Cadmium adsorption on vermiculite, zeolite and pumice: Batch experimental studies

Maria Rosaria Panuccio et al.

JOURNAL OF ENVIRONMENTAL MANAGEMENT (2009)

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Article Plant Sciences

The relationship of drought-related gene expression in Arabidopsis thaliana to hormonal and environmental factors

Daiqing Huang et al.

JOURNAL OF EXPERIMENTAL BOTANY (2008)

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Article Plant Sciences

Effects of salt stress in relation to osmotic adjustment on sugarcane (Saccharum officinarum L.) callus cultures

Vikas Yadav Patade et al.

PLANT GROWTH REGULATION (2008)

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Article Plant Sciences

Low temperature stress-iniduced biochemical changes affect stubble bud sprouting in sugarcane (Saccharum spp. hybrid)

Radha Jain et al.

PLANT GROWTH REGULATION (2007)

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Article Agronomy

Targeted mapping of a sugarcane rust resistance gene (Bru1) using bulked segregant analysis and AFLP markers

C Asnaghi et al.

THEORETICAL AND APPLIED GENETICS (2004)

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Article Agronomy

Elimination of Sugarcane yellow leaf virus from infected sugarcane plants by meristem tip culture visualized by tissue blot immunoassay

MMM Fitch et al.

PLANT PATHOLOGY (2001)

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