Seroprevalence and Factors Associated with Toxoplasma gondii, Neospora caninum, and Besnoitia besnoiti Infections in Cattle and Goats in Selangor, Malaysia

Simple Summary Toxoplasma gondii, Neospora caninum, and Besnoitia besnoiti are important causes of production losses in small and large ruminants. These parasites could result in severe economic losses to farmers if they are not effectively controlled. The prevalence of these parasites in smallholder farms in Malaysia is underreported. Thus, this study attempted to determine the level of exposure of cattle and goat population from 19 farms in Selangor, Malaysia to T. gondii, N. caninum, and B. besnoiti using ELISA test kits. Results revealed that the prevalence of T. gondii, N. caninum, and B. besnoiti antibodies in the sampled cattle was 5.3%, 2.5%, and 5.7%, respectively. The corresponding seroprevalence in the sampled goats was 69.8% for T. gondii and 3.9% for N. caninum antibodies. Further analyses demonstrated that older animals, semi-intensive management systems, the presence of dogs or cats on farms, a large herd size (>100 animals), and the source of replacement stock increased the risk of exposure to T. gondii. These findings reflect the extent of T. gondii, N. caninum, and B. besnoiti infections in smallholder farms in Selangor. More research is required to develop effective measures to control these important parasites at the national level. Apicomplexan parasites such as Toxoplasma gondii, Neospora caninum, and Besnoitia besnoiti are widely recognized as causes of production diseases in ruminants. This study aimed to investigate the serological occurrence of T. gondii, N. caninum, and B. besnoiti in cattle and goats from smallholder farms in Selangor, Malaysia. A cross-sectional study was conducted on 19 farms by collecting 404 bovine (n = 225) and caprine (n = 179) serum samples, which were then essayed for T. gondii, N. caninum, and B. besnoiti antibodies using commercially available ELISA test kits. Farm data and animal characteristics were documented, and the data were analyzed using descriptive statistics and logistic regression models. The seroprevalence of T. gondii at animal and farm levels in cattle was 5.3% (95% CI 1.2-7.4%) and 36.8% (95% CI 22.4-58.0%), respectively. Animal-level seropositivity for N. caninum was 2.7% (95% CI 0.4-4.2%) and 5.7% for B. besnoiti (95% CI 1.3-9.4%) with corresponding farm-level seropositivity of 21.0% and 31.5%, respectively. For the goat samples, a high animal- (69.8%; 95% CI 34.1-82.0%) and farm-level (92.3%) seropositivity was recorded for T. gondii, but was relatively lower for N. caninum antibodies, at 3.9% (95% CI 1.5-6.2%) and 38.4% (5/13). The factors associated with T. gondii seropositivity were older animals (above 12 months) (OR = 5.3; 95% CI 1.7-16.6), semi-intensive farms (OR = 2.2; 95% CI 1.3-6.2), the presence of either dogs or cats (OR = 3.6; 95% CI 1.1-12.3), a large herd size (>100 animals) (OR = 3.7; 95% CI 1.4-10.0), and a single source of replacement animals (OR = 3.9; 95% CI 1.6-9.6). These findings are vital in developing effective control measures against these parasites in ruminant farms in Selangor, Malaysia. More national epidemiological research is required to elucidate the spatial distribution of these infections and their potential impact on Malaysia's livestock industry.

Automated summary

Toxoplasma gondii, Neospora caninum, and Besnoitia besnoiti are important parasites causing production losses in ruminants. This study investigated the seroprevalence of these parasites in cattle and goats from smallholder farms in Selangor, Malaysia. The results showed a significant presence of T. gondii, N. caninum, and B. besnoiti antibodies in both cattle and goats, highlighting the need for effective control measures at the national level.