Short-Term Thermal Stress Affects Immune Cell Features in the Sea Urchin Paracentrotus lividus

Simple Summary The immune response to pathogens is one of the organic functions at risk due to climatic change. The effects of a short-term increase in temperature on immune cell functions have been evaluated using a simple animal model, such as the sea urchin, exposed to temperatures above its comfort zone (17 & DEG;C), causing moderate and severe heat stress. After 3 and 7 days of exposure to the three temperatures tested, the coelomic fluid of the animals was collected. The coelomocytes were isolated and typed and the mitochondrial activity, the amount of lipid peroxidation, and the production of hydrogen peroxide were evaluated. These cells were also stimulated with phorbol 12-myristate 13-acetate (PMA) to evoke respiratory burst, a marker of immune response. Heat stress altered both the distribution of the various types of coelomocytes and their mitochondrial activity; it also increased lipid peroxidation and reduced the production of hydrogen peroxide. The respiratory burst occurred in the coelomocytes of all three experimental groups; however, it was greater in the group kept at 17 & DEG;C. In conclusion, thermal increase alters immune cell functions with possible repercussions on defense mechanisms against pathogens. Due to global warming, animals are experiencing heat stress (HS), affecting many organic functions and species' survival. In this line, some characteristics of immune cells in sea urchins subjected to short-term HS were evaluated. Paracentrotus lividus adult females were randomly divided into three groups and housed in tanks at 17 & DEG;C. In two of these tanks, the temperatures were gradually increased up to 23 and 28 & DEG;C. Celomatic fluid was collected after 3 and 7 days. The coelomocytes were morphologically typed and evaluated for their mitochondrial membrane potential (MMP), lipoperoxidation extent (LPO), and hydrogen peroxide content (H2O2). Respiratory burst was induced by treatment with phorbol 12-myristate 13-acetate (PMA). HS caused a significant change in the coelomocytes' type distribution. MMP increased in the 23 & DEG;C-group and decreased in the 28 & DEG;C-group at both 3 and 7 days. LPO only increased in the 28 & DEG;C-group at 7 days. H2O2 progressively decreased together with the temperature increase. Respiratory burst was detected in all groups, but it was higher in the 17 & DEG;C group. In conclusion, the increase in temperature above the comfort zone for this animal species affects their immune cells with possible impairment of their functions.

Automated summary

Climate change poses a risk to the immune response against pathogens. A study on sea urchins exposed to temperatures above their comfort zone found that heat stress altered the distribution and activity of immune cells, increased lipid peroxidation, and reduced hydrogen peroxide production. The respiratory burst, a marker of immune response, was observed in all groups but was higher in the group kept at the optimal temperature. These findings suggest that high temperatures can impair immune cell functions and affect defense mechanisms against pathogens.