期刊
INSECTS
卷 14, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/insects14050423
关键词
biological control; natural enemy; mass rearing; ultraviolet radiation; increasing efficiency
类别
This study investigated the effects of ultraviolet-B (UVB) radiation on laboratory-reared Drosophila melanogaster pupae and its impact on Trichopriadrosophilae. The results showed that UVB radiation significantly reduced host emergence and affected the development duration of the parasitoid. This has important implications for the separation of hosts and parasitoids as well as the separation of females and males.
The pupal parasitoid, Trichopria drosophilae Perkins (Hymenoptera: Diapriidae), is an ectoparasitoid of the genus Drosophila with great potential for application in biological control based on its excellent control efficiency for Drosophila suzukii Matsumura (Diptera: Drosophilidae), and it has has even been commercialized by biofactories. Due to its characteristics of short life cycle, large number of offspring, easy rearing, rapid reproduction, and low cost, Drosophila melanogaster (Diptera: Drosophilidae) is currently being utilized as a host to mass produce T. drosophilae. To simplify the mass rearing process and omit the separation of hosts and parasitoids, ultraviolet-B (UVB) was used as an irradiation source to irradiate D. melanogaster pupae, and the effects on T. drosophilae were studied. The results showed that UVB radiation significantly reduces host emergence and affects the duration of parasitoid development (female: F0 increased from 21.50 to 25.80, F1 from 23.10 to 26.10; male: F0 decreased from 17.00 to 14.10, F1 from 17.20 to 14.70), which has great significance for the separation of hosts and parasitoids as well as of females and males. Of the various studied conditions, UVB irradiation was ideal when the host was supplied with parasitoids for 6 h. The selection test results showed that the female-to-male ratio of emerging parasitoids in this treatment was highest at 3.47. The no-selection test resulted in the highest rates of parasitization and parasitoid emergence rate, maximized inhibition of host development, and allowed the omission of the separation step. Finally, the results of the semi-field test showed that the parasitoids bred in this treatment could search for their hosts normally and could therefore be directly applied in the biological control of Drosophila pests in the field.
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