Neuroprotective Activities of New Monoterpenoid Indole Alkaloid from Nauclea officinalis

Phytochemical investigation on the bark of Nauclea officinalis led to the isolation of a new monoterpenoid indole alkaloid, nauclediol. The structure of the compound was identified through extensive spectroscopic analysis. Nauclediol displayed cholinesterase-inhibitory activities towards AChE and BChE with IC50 values of 15.429 and 8.756 mu M, respectively. Statistical analysis revealed that the mode of inhibition of nauclediol was non-competitive inhibitor for both AChE and BChE. Molecular docking revealed that nauclediol interacts with the choline-binding site and the catalytic triad of TcAChE and hBChE. This study also demonstrated the neuroprotective potential of nauclediol against amyloid beta-induced cytotoxicity and LPS-induced neuroinflammation activity in a dose-dependent manner.

Automated summary

Phytochemical investigation on the bark of Nauclea officinalis led to the discovery of a new monoterpenoid indole alkaloid, nauclediol. Its structure was identified through extensive spectroscopic analysis. Nauclediol showed cholinesterase-inhibitory activities against AChE and BChE, with IC50 values of 15.429 and 8.756 μM, respectively. Statistical analysis revealed that nauclediol acts as a non-competitive inhibitor for both AChE and BChE. Molecular docking studies showed that nauclediol interacts with the choline-binding site and the catalytic triad of TcAChE and hBChE. This study also demonstrated the neuroprotective potential of nauclediol against amyloid beta-induced cytotoxicity and LPS-induced neuroinflammation activity in a dose-dependent manner.