期刊
CELLS
卷 12, 期 13, 页码 -出版社
MDPI
DOI: 10.3390/cells12131689
关键词
retina; age-related macular degeneration; retinitis pigmentosa; photoreceptor; retinal pigmented epithelium; retinal degeneration; secretome; Royal College of Surgeons rat
类别
This study explored the use of human embryonic stem cell-derived, polarized retinal pigment epithelium soluble factors (PRPE-SF) as a combination treatment for retinal degenerative diseases. PRPE-SF promoted retinal progenitor cell survival and reduced oxidative stress, demonstrating potential for delaying retinal degeneration. Importantly, PRPE-SF treatment preserved retinal structure and function, offering a promising therapeutic candidate for these debilitating conditions.
Retinal degenerative diseases, including age-related macular degeneration (AMD) and retinitis pigmentosa, lack effective therapies. Conventional monotherapeutic approaches fail to target the multiple affected pathways in retinal degeneration. However, the retinal pigment epithelium (RPE) secretes several neurotrophic factors addressing diverse cellular pathways, potentially preserving photoreceptors. This study explored human embryonic stem cell-derived, polarized RPE soluble factors (PRPE-SF) as a combination treatment for retinal degeneration. PRPE-SF promoted retinal progenitor cell survival, reduced oxidative stress in ARPE-19 cells, and demonstrated critical antioxidant and anti-inflammatory effects for preventing retinal degeneration in the Royal College of Surgeons (RCS) rat model. Importantly, PRPE-SF treatment preserved retinal structure and scotopic b-wave amplitudes, suggesting therapeutic potential for delaying retinal degeneration. PRPE-SF is uniquely produced using biomimetic membranes for RPE polarization and maturation, promoting a protective RPE secretome phenotype. Additionally, PRPE-SF is produced without animal serum to avoid immunogenicity in future clinical development. Lastly, PRPE-SF is a combination of neurotrophic factors, potentially ameliorating multiple dysfunctions in retinal degenerations. In conclusion, PRPE-SF offers a promising therapeutic candidate for retinal degenerative diseases, advancing the development of effective therapeutic strategies for these debilitating conditions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据