Transition from Animal-Based to Human Induced Pluripotent Stem Cells (iPSCs)-Based Models of Neurodevelopmental Disorders: Opportunities and Challenges

Neurodevelopmental disorders (NDDs) arise from the disruption of highly coordinated mechanisms underlying brain development, which results in impaired sensory, motor and/or cognitive functions. Although rodent models have offered very relevant insights to the field, the translation of findings to clinics, particularly regarding therapeutic approaches for these diseases, remains challenging. Part of the explanation for this failure may be the genetic differences-some targets not being conserved between species-and, most importantly, the differences in regulation of gene expression. This prompts the use of human-derived models to study NDDS. The generation of human induced pluripotent stem cells (hIPSCs) added a new suitable alternative to overcome species limitations, allowing for the study of human neuronal development while maintaining the genetic background of the donor patient. Several hIPSC models of NDDs already proved their worth by mimicking several pathological phenotypes found in humans. In this review, we highlight the utility of hIPSCs to pave new paths for NDD research and development of new therapeutic tools, summarize the challenges and advances of hIPSC-culture and neuronal differentiation protocols and discuss the best way to take advantage of these models, illustrating this with examples of success for some NDDs.

Automated summary

Neurodevelopmental disorders (NDDs) result from disruptions in brain development, leading to impaired sensory, motor, and/or cognitive functions. While rodent models have provided valuable insights, translating findings to clinical therapies remains challenging. Human-induced pluripotent stem cells (hIPSCs) have emerged as a promising tool for NDD research, allowing for the study of human neuronal development and disease-specific phenotypes. This review highlights the utility of hIPSCs in advancing NDD research and discusses the challenges and advances in hIPSC culture and neuronal differentiation protocols.