期刊
CELLS
卷 12, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/cells12091328
关键词
Aplodinotus grunniens; tissue-specific genes; feed-restriction; cold stress; abdominal fat
类别
In this study, reference genes and tissue-specific genes were identified in the adipose tissue of Aplodinotus grunniens, and their function under exogenous starvation and hypothermic stress was explored. Results showed that 60SRP was the most stable reference gene in adipose tissue. In addition, seven tissue-specific genes were confirmed in adipose tissue and were found to be active in response to starvation and hypothermic stress in a time- or temperature-dependent manner. These findings provide tissue-specific targets for adipose regulation in A. grunniens.
Adipose tissue is critical to the growth, development, and physiological health of animals. Reference genes play an essential role in normalizing the expression of mRNAs. Tissue-specific genes are preferred for their function and expression in specific tissues or cell types. Identification of these genes contributes to understanding the tissue-gene relationship and the etiology and discovery of new tissue-specific targets. Therefore, reference genes and tissue-specific genes in the adipose tissue of Aplodinotus grunniens were identified to explore their function under exogenous starvation (1 d, 2 w, 6 w) and hypothermic stress (18 degrees C and 10 degrees C for 2 d and 8 d) in this study. Results suggest that 60SRP was the most stable reference gene in adipose tissue. Meanwhile, eight genes were validated as tissue-specific candidates from the high-throughput sequencing database, while seven of them (ADM2, beta(2)GP1, CAMK1G, CIDE3, FAM213A, HSL, KRT222, and NCEH1) were confirmed in adipose tissue. Additionally, these seven tissue-specific genes were active in response to starvation and hypothermic stress in a time- or temperature-dependent manner. These results demonstrate that adipose-specific genes can be identified using stable internal reference genes, thereby identifying specific important functions under starvation and hypothermic stress, which provides tissue-specific targets for adipose regulation in A. grunniens.
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