期刊
CELLS
卷 12, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/cells12091291
关键词
synthetic carrier; organic synthesis; endosomal escape; hydrophobic moieties; engineered protein
类别
Many molecular targets for cancer therapy are located in the cytosol, but therapeutic macromolecules generally cannot spontaneously translocate across membranes to reach these targets. To enhance cytosolic delivery, researchers have used Shiga toxin B-subunit (STxB) to deliver therapeutic principles to disease-relevant cells expressing its receptor, Gb3. By introducing unnatural amino acids and functionalizing them with hydrophobic entities, the membrane translocation of STxB was increased by a factor of 2.5, opening up new biomedical opportunities.
Many molecular targets for cancer therapy are located in the cytosol. Therapeutic macromolecules are generally not able to spontaneously translocate across membranes to reach these cytosolic targets. Therefore a strong need exists for tools that enhance cytosolic delivery. Shiga toxin B-subunit (STxB) is used to deliver therapeutic principles to disease-relevant cells that express its receptor, the glycolipid Gb3. Based on its naturally existing membrane translocation capacity, STxB delivers antigens to the cytosol of Gb3-positive dendritic cells, leading to the induction of CD8(+) T cells. Here, we have explored the possibility of further increasing the membrane translocation of STxB to enable other therapeutic applications. For this, our capacity to synthesize STxB chemically was exploited to introduce unnatural amino acids at different positions of the protein. These were then functionalized with hydrophobic entities to locally destabilize endosomal membranes. Intracellular trafficking of these functionalized STxB was measured by confocal microscopy and their cytosolic arrival with a recently developed highly robust, sensitive, and quantitative translocation assay. From different types of hydrophobic moieties that were linked to STxB, the most efficient configuration was determined. STxB translocation was increased by a factor of 2.5, paving the path for new biomedical opportunities.
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