CircXPO1 Promotes Glioblastoma Malignancy by Sponging miR-7-5p

Mounting evidence suggests that circular RNAs play important roles in the development and progression of cancers. However, their function in glioblastomas (GBM) is still unclear. By circRNA array analysis, we found that circXPO1 (hsa_circ_102737) was significantly upregulated in GBM, and qPCR analysis verified that the circXPO1 expression level was increased in both GBM tissues and cell lines. Functional studies demonstrated that the knockdown of circXPO1 in GBM cell lines repressed cell proliferation and migration; conversely, the overexpression of circXPO1 promoted the malignancy of GBM cells. In line with these findings, circXPO1 inhibition effectively suppressed gliomagenesis in the in situ transplantation model of nude mice. Through bioinformatic analyses and dual-luciferase reporter assays, we showed that circXPO1 directly bound to miR-7-5p, which acted as a tumor suppressor through the negative regulation of RAF1. In conclusion, our studies suggest that the circXPO1/miR-7-5p/RAF1 axis promotes brain tumor formation and may be a potential therapeutic target for GBM treatment.

Automated summary

Mounting evidence suggests that circular RNAs (circRNAs) play important roles in the development and progression of cancers. In this study, researchers identified a specific circRNA, circXPO1, that was significantly upregulated in glioblastomas (GBM). Functional studies demonstrated that circXPO1 promoted GBM cell proliferation, migration, and tumor formation. Further investigation revealed that circXPO1 directly bound to miR-7-5p, which negatively regulated the RAF1 gene expression. These findings suggest that circXPO1 may be a potential therapeutic target for GBM treatment.