期刊
CELLS
卷 12, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/cells12040611
关键词
ferroptosis; mitochondria; complex I; mtROS; mitochondria targeted antioxidants
类别
Ferroptosis induced by erastin and butionine sulfoximine can be prevented by the mitochondria-targeted antioxidants SkQ1 and MitoTEMPO. This prevention is associated with the inhibition of mitochondrial lipid peroxidation, which occurs before cell death. Methylene blue, a redox agent that inhibits reactive oxygen species (ROS) production in complex I of the mitochondrial electron transport chain, also inhibits ferroptosis and mitochondrial lipid peroxidation. We propose that ROS generated in complex I promote mitochondrial lipid peroxidation and ferroptosis.
Ferroptosis induced by erastin (an inhibitor of cystine transport) and butionine sulfoximine (an inhibitor of glutathione biosynthesis) was prevented by the mitochondria-targeted antioxidants SkQ1 and MitoTEMPO. These effects correlate with the prevention of mitochondrial lipid peroxidation, which precedes cell death. Methylene blue, a redox agent that inhibits the production of reactive oxygen species (ROS) in complex I of the mitochondrial electron transport chain, also inhibits ferroptosis and mitochondrial lipid peroxidation. Activation of ROS production in complex I with rotenone in the presence of ferrous iron stimulates lipid peroxidation in isolated mitochondria, while ROS produced by complex III are ineffective. SkQ1 and methylene blue inhibit lipid peroxidation. We suggest that ROS formed in complex I promote mitochondrial lipid peroxidation and ferroptosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据