Detection and Molecular Characterization of Circulating Tumour Cells: Challenges for the Clinical Setting

Over the last decade, liquid biopsy has gained much attention as a powerful tool in personalized medicine since it enables monitoring cancer evolution and follow-up of cancer patients in real time. Through minimally invasive procedures, liquid biopsy provides important information through the analysis of circulating tumour cells (CTCs) and circulating tumour-derived material, such as circulating tumour DNA (ctDNA), circulating miRNAs (cfmiRNAs) and extracellular vehicles (EVs). CTC analysis has already had an important impact on the prognosis, detection of minimal residual disease (MRD), treatment selection and monitoring of cancer patients. Numerous clinical trials nowadays include a liquid biopsy arm. CTC analysis is now an exponentially expanding field in almost all types of solid cancers. Functional studies, mainly based on CTC-derived cell-lines and CTC-derived explants (CDx), provide important insights into the metastatic process. The purpose of this review is to summarize the latest findings on the clinical significance of CTCs for the management of cancer patients, covering the last four years. This review focuses on providing a comprehensive overview of CTC analysis in breast, prostate and non-small-cell lung cancer. The unique potential of CTC single-cell analysis for understanding metastasis biology, and the importance of quality control and standardization of methodologies used in this field, is also discussed.

Automated summary

Liquid biopsy, through the analysis of circulating tumour cells (CTCs) and tumour-derived material, has become a powerful tool in personalized medicine for monitoring cancer evolution and follow-up of cancer patients. CTC analysis has had a significant impact on cancer prognosis, minimal residual disease detection, treatment selection, and patient monitoring, with numerous clinical trials including a liquid biopsy arm. This review provides an overview of the clinical significance of CTCs in breast, prostate, and non-small-cell lung cancer, and highlights the potential of CTC single-cell analysis for understanding metastasis biology and the need for quality control and standardization of methodologies in this field.