Imaging of Escherichia coli K5 and glycosaminoglycan precursors via targeted metabolic labeling of capsular polysaccharides in bacteria

Review Chemistry, Multidisciplinary

Chemical Reporters for Bacterial Glycans: Development and Applications

Nicholas Banahene et al.

Summary: This article reviews the physiological functions of bacterial glycans and the development and applications of chemical reporters targeting bacterial glycans, including their use in diagnostics, therapeutics, and research.

CHEMICAL REVIEWS (2022)

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Article Chemistry, Physical

Heparosan oligosaccharide synthesis using engineered single-function glycosyltransferases

Jian-Qun Deng et al.

Summary: This study presents a biocatalytic approach for the size-controlled synthesis of heparosan oligosaccharides using two single-function glycosyltransferases. By screening and modifying enzymes, the researchers successfully synthesized size-defined heparosan oligosaccharides with minimized byproduct formation.

CATALYSIS SCIENCE & TECHNOLOGY (2022)

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Article Multidisciplinary Sciences

Structure, substrate recognition and initiation of hyaluronan synthase

Finn P. Maloney et al.

Summary: In this study, we investigated the substrate binding and polymer synthesis of hyaluronan synthase (HAS) using cryo-electron microscopy structures, biochemical analyses, and molecular dynamics simulations. Our findings provide detailed insights into the transmembrane process of HAS, including substrate selection, polymerization, and regulation of hyaluronan synthesis. This research is important for understanding the biosynthesis of acidic extracellular polysaccharides and one of the most abundant glycosaminoglycans in the human body.

NATURE (2022)

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Article Biochemistry & Molecular Biology

Chemical editing of proteoglycan architecture

Timothy R. O'Leary et al.

Summary: Proteoglycans are important glycoconjugates that play a role in many cellular processes. However, the study of their structure and function has mainly focused on the glycosaminoglycan chains, overlooking other important elements such as core proteins and membrane localization. In this study, we present a comprehensive approach to investigate proteoglycan structure and biology by creating tailored semisynthetic modular proteoglycans that can be displayed on the cell surface. By using proteoglycan core proteins with unnatural amino acids, we were able to systematically study the binding and function of various proteoglycan-binding proteins.

NATURE CHEMICAL BIOLOGY (2022)

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Article Chemistry, Analytical

Coupling Liquid Chromatography and Tandem Mass Spectrometry to Electrophoresis for In-Depth Analysis of Glycosaminoglycan Drugs: Heparin and the Multicomponent Sulodexide

Anran Sheng et al.

Summary: Glycosaminoglycans (GAGs) play a role in the treatment of many human diseases, especially in thrombosis, due to their anticoagulant activity. Novel electrophoresis-mass spectrometry methods have been developed to directly elucidate the structures of larger GAG chains with more biological importance, aiding in understanding the structure-activity relationship and quality control of GAG drugs.

ANALYTICAL CHEMISTRY (2021)

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Article Ophthalmology

Efficacy and Safety of Sodium Hyaluronate/chondroitin Sulfate Preservative-free Ophthalmic Solution in the Treatment of Dry Eye: A Clinical Trial

Sandra Belalcazar-Rey et al.

Summary: The study evaluated the efficacy of a preservative free sodium hyaluronate/chondroitin sulfate ophthalmic solution in patients with dry eye disease. Results showed that the solution can improve eye health and symptoms in patients with dry eye disease, with similar effectiveness and safety compared to conventional eye drops.

CURRENT EYE RESEARCH (2021)

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Review Chemistry, Multidisciplinary

Design of Polymeric Carriers for Intracellular Peptide Delivery in Oncology Applications

Shixian Lv et al.

Summary: Peptides as promising cancer therapeutics have shown robust antitumor efficacy, but the translation into clinical therapies is hindered by inherent limitations in their structure, hindering intracellular delivery. Strategies to engineer polymeric materials for increased peptide delivery efficiency, especially cytosolic delivery, play a crucial role in potentiating peptide-based cancer therapies. Future opportunities for peptides in cancer treatment lie in the design of polymer nanocarriers for optimized peptide delivery.

CHEMICAL REVIEWS (2021)

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Review Biochemistry & Molecular Biology

Applications of Hyaluronic Acid in Ophthalmology and Contact Lenses

Wan-Hsin Chang et al.

Summary: Hyaluronic acid (HA) is commonly used in ophthalmology for its biological safety and water retention properties. It is often utilized in intravitreal injections, dry eye treatment, and contact lenses. HA in contact lenses can improve wearer comfort and gradually release to the eyes to treat dry eye.

MOLECULES (2021)

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Article Chemistry, Analytical

Glycan Labeling and Analysis in Cells and In Vivo

Bo Cheng et al.

Summary: Glycans, as one of the major biomacromolecules in cells, are crucial in various biological processes. Recent advances in labeling, imaging, and profiling of glycans have enabled researchers to better understand their biological functions in physiological or pathological contexts. These methods and strategies have facilitated the visualization, tracking, and enrichment of glycans, as well as the selective labeling and imaging of glycans in cells and tissues.

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 14, 2021 (2021)

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Review Chemistry, Applied

Hyaluronic acid in ocular drug delivery

Xiaodan Zhang et al.

Summary: Hyaluronic acid, a naturally-occurring polysaccharide in ophthalmic tissues, has a wide range of applications in the treatment of ocular diseases. Recent advances in HA-based drug delivery systems have shown the importance of rational selection of molecular weight and amount of HA for diverse delivery systems.

CARBOHYDRATE POLYMERS (2021)

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Article Biochemical Research Methods

Comprehensive analysis of glycosphingolipid glycans by lectin microarrays and MALDI-TOF mass spectrometry

Haoqi Du et al.

Summary: The protocol provides a step-by-step approach to analyzing GSL glycans from cultured cells using a lectin microarray method, which is high-throughput, low-cost, and provides detailed information about glycan linkages.

NATURE PROTOCOLS (2021)

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Article Microbiology

Heterologous Hyaluronic Acid Production in Kluyveromyces lactis

Antonio M. V. Gomes et al.

MICROORGANISMS (2019)

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Article Biotechnology & Applied Microbiology

Identification and characterization of a chondroitin synthase from Avibacterium paragallinarum

Ting-Ting Wang et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2018)

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Review Biochemical Research Methods

The road to animal-free glycosaminoglycan production: current efforts and bottlenecks

Abinaya Badri et al.

CURRENT OPINION IN BIOTECHNOLOGY (2018)

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Article Biotechnology & Applied Microbiology

Metabolic engineering to enhance heterologous production of hyaluronic acid in Bacillus subtilis

Adam W. Westbrook et al.

METABOLIC ENGINEERING (2018)

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Article Biotechnology & Applied Microbiology

KfoA, the UDP-glucose-4-epimerase of Escherichia coli strain O5:K4:H4, shows preference for acetylated substrates

Hao-Miao Zhu et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2018)

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Article Chemistry, Medicinal

Enzymatic Generation of Highly Anticoagulant Bovine Intestinal Heparin

Li Fu et al.

JOURNAL OF MEDICINAL CHEMISTRY (2017)

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Article Engineering, Biomedical

Design and optimization of a novel implantation technology in contact lenses for the treatment of dry eye syndrome: In vitro and in vivo evaluation

Furgan A. Maulvi et al.

ACTA BIOMATERIALIA (2017)

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Article Chemistry, Multidisciplinary

Metabolic Labeling and Imaging of N-Linked Glycans in Arabidopsis Thaliana

Yuntao Zhu et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2016)

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Article Biochemistry & Molecular Biology

Uncovering the Catalytic Direction of Chondroitin AC Exolyase FROM THE REDUCING END TOWARDS THE NON-REDUCING END

Feng-Xin Yin et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2016)

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Article Biochemistry & Molecular Biology

Preparation of Low Molecular Weight Chondroitin Sulfates, Screening of a High Anti-Complement Capacity of Low Molecular Weight Chondroitin Sulfate and Its Biological Activity Studies in Attenuating Osteoarthritis

Lian Li et al.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2016)

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Article Chemistry, Analytical

Enantioselective analysis of D- and L-amino acids from mouse macrophages using high performance liquid chromatography

Shiro Kato et al.

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS (2015)

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Review Microbiology

Illumination of growth, division and secretion by metabolic labeling of the bacterial cell surface

M. Sloan Siegrist et al.

FEMS MICROBIOLOGY REVIEWS (2015)

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Article Biochemical Research Methods

Quantitation of heparosan with heparin lyase III and spectrophotometry

Haichan Huang et al.

ANALYTICAL BIOCHEMISTRY (2014)

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Article Biotechnology & Applied Microbiology

Donor substrate promiscuity of the N-acetylglucosaminyltransferase activities of Pasteurella multocida heparosan synthase 2 (PmHS2) and Escherichia coli K5 KfiA

Yanhong Li et al.

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2014)

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Review Biochemistry & Molecular Biology

Synthetic probes of glycosaminoglycan function

Matthew E. Griffin et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2013)

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Review Biochemistry & Molecular Biology

Chemical reporters for biological discovery

Markus Grammel et al.

NATURE CHEMICAL BIOLOGY (2013)

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Article Biochemical Research Methods

Analysis of E. coli K5 capsular polysaccharide heparosan

Mellisa Ly et al.

ANALYTICAL AND BIOANALYTICAL CHEMISTRY (2011)

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Article Chemistry, Multidisciplinary

One-pot three-enzyme synthesis of UDP-GlcNAc derivatives

Yi Chen et al.

CHEMICAL COMMUNICATIONS (2011)

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Article Biochemistry & Molecular Biology

Substrate Promiscuity of N-Acetylhexosamine 1-Kinases

Yanhong Li et al.

MOLECULES (2011)

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Article Biochemical Research Methods

Bringing chemistry to life

Michael Boyce et al.

NATURE METHODS (2011)

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Article Chemistry, Multidisciplinary

Highly Efficient Synthesis of UDP-GalNAc/GlcNAc Analogues with Promiscuous Recombinant Human UDP-GalNAc Pyrophosphorylase AGX1

Wanyi Guan et al.

CHEMISTRY-A EUROPEAN JOURNAL (2010)

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Article Multidisciplinary Sciences

Targeted metabolic labeling of yeast N-glycans with unnatural sugars

Mark A. Breidenbach et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)

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Article Multidisciplinary Sciences

Copper-free click chemistry in living animals

Pamela V. Chang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)

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Article Biochemistry & Molecular Biology

Lectin microarrays identify cell-specific and functionally significant cell surface glycan markers

Sheng-Ce Tao et al.

GLYCOBIOLOGY (2008)

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Article Multidisciplinary Sciences

Glycoproteomic probes for fluorescent imaging of fucosylated glycans in vivo

Masaaki Sawa et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2006)

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Article Biochemistry & Molecular Biology

Analyzing the dynamic bacterial glycome with a lectin microarray approach

KL Hsu et al.

NATURE CHEMICAL BIOLOGY (2006)

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Article Orthopedics

Osteoarthritis development in novel experimental mouse models induced by knee joint instability

S Kamekura et al.

OSTEOARTHRITIS AND CARTILAGE (2005)

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Review Cell Biology

Heparan sulphate proteoglycans:: The sweet side of development

U Häcker et al.

NATURE REVIEWS MOLECULAR CELL BIOLOGY (2005)

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Article Multidisciplinary Sciences

A metabolic labeling approach toward proteomic analysis of mucin-type O-linked glycosylation

HC Hang et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)

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Article Multidisciplinary Sciences

A chemical approach for identifying O-GlcNAc-modified proteins in cells

DJ Vocadlo et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2003)

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Review Oncology

Roles of heparan-sulphate glycosaminoglycans in cancer

R Sasisekharan et al.

NATURE REVIEWS CANCER (2002)

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Article Biochemistry & Molecular Biology

Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4

T Ninomiya et al.

JOURNAL OF BIOLOGICAL CHEMISTRY (2002)

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Imaging of Escherichia coli K5 and glycosaminoglycan precursors via targeted metabolic labeling of capsular polysaccharides in bacteria

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