4.7 Article

Effects of Bifidobacteria bifidum strains on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced acute colitis and its potential mechanism

期刊

FOOD BIOSCIENCE
卷 52, 期 -, 页码 -

出版社

ELSEVIER
DOI: 10.1016/j.fbio.2023.102387

关键词

Bifidobacteria bifidum; TNBS colitis; Gut microbiota; Immunomodulatory; Adhesion

向作者/读者索取更多资源

Microbiota-based therapies, such as probiotics, are being explored for improving symptoms of Crohn's disease (CD). In this study, eight strains of B. bifidum with potential for relieving CD symptoms were screened and CJ238 strain was found to prevent acute colitis in mice. Moreover, heat-killed B. bifidum cells also protected mice against acute colitis.
Microbiota-based therapies for improving symptoms of Crohn's disease (CD) are receiving increasing attention. Probiotics and second-generation probiotics can potentially prevent this disease. However, the therapeutic action and underlying molecular mechanisms of probiotics remain largely unknown. Efficient screening of probiotics is also challenging. In this study, we screened eight B. bifidum strains with CD-relieving potential using a 2,4,6-tri-nitrobenzene sulfonic acid (TNBS) colitis mouse model. We also observed that B. bifidum CJ238 could prevent but not cure the colitis-symptoms (weight loss, colon shortening and connective tissue hyperplasia) in mice model. The 16S rRNA sequencing data showed that supplementation with B. bifidum did not alter the composition of the intestinal microbiota. B. bifidum CJ238 administration also provided full protection against acute colitis in pseudo germ-free mice. Therefore, we infered that the protective effect of B. bifidum strains does not depend on their interaction with the gut microbiota. Furthermore, heat-killed (95 degrees C for 20min) B. bifidum cells (109 CFU/ mouse) also protected mice against acute colitis. In-vitro adhesion and immunomodulatory activities of the B. bifidum strains were measured. Combined with the in-vivo results, the anti-colitis effect of B. bifidum was positively associated with its strong anti-inflammatory and adhesion capacity in-vitro. This study provides in-sights into the in-vitro screening and clinical application of probiotics in CD prevention.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据