期刊
FRONTIERS IN IMMUNOLOGY
卷 14, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2023.1083339
关键词
megakaryocyte; platelet; sepsis; innate; infectious
类别
Histological studies of sepsis patients found increased MKs in the lungs and platelets within microthrombi in the kidneys, which correlated with organ dysfunction. Imaging cytometry of peripheral blood from sepsis patients showed significantly higher MK counts, likely misclassified as leukocytes by automated analyzers. In vitro techniques revealed that MKs interact with bacteria, undergo chemotaxis, and release chromatin webs in response to pathogenic stimuli, suggesting their involvement in sepsis.
Megakaryocytes (MKs) are precursors to platelets, the second most abundant cells in the peripheral circulation. However, while platelets are known to participate in immune responses and play significant functions during infections, the role of MKs within the immune system remains largely unexplored. Histological studies of sepsis patients identified increased nucleated CD61(+) cells (MKs) in the lungs, and CD61(+) staining (likely platelets within microthrombi) in the kidneys, which correlated with the development of organ dysfunction. Detailed imaging cytometry of peripheral blood from patients with sepsis found significantly higher MK counts, which we predict would likely be misclassified by automated hematology analyzers as leukocytes. Utilizing in vitro techniques, we show that both stem cell derived MKs (SC MKs) and cells from the human megakaryoblastic leukemia cell line, Meg-01, undergo chemotaxis, interact with bacteria, and are capable of releasing chromatin webs in response to various pathogenic stimuli. Together, our observations suggest that MK cells display some basic innate immune cell behaviors and may actively respond and play functional roles in the pathophysiology of sepsis.
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