期刊
FRONTIERS IN IMMUNOLOGY
卷 13, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fimmu.2022.978862
关键词
colorectal cancer; prognosis; dendritic cells; T cells; cancer immunology
类别
Colorectal cancer (CRC) is a leading cause of cancer-related deaths, and the presence of tumour-infiltrating leukocytes has a significant impact on prognosis. Three computational methods were used to predict immune cell types in CRC tissue and their abundance for assessing prognosis. The results showed significant differences in immune cell composition between CRC and normal colon tissue, with dendritic cells and mast cells identified as positive prognostic markers. The analysis also revealed that immune cell composition has a more pronounced effect on prognosis in early-stage CRC compared to late-stage CRC. Overall, characterizing the immune landscape in CRC provides a valuable tool for evaluating prognosis and facilitating the use of immunotherapies.
IntroductionColorectal cancer (CRC) is the third leading cause of cancer-related deaths globally. Tumour-infiltrating leukocytes play an important role in cancers, including CRC. We therefore sought to characterize the impact of tumour-infiltrating leukocytes on CRC prognosis. MethodsTo determine whether the immune cell profile within CRC tissue could influence prognosis, we employed three computational methodologies (CIBERSORT, xCell and MCPcounter) to predict abundance of immune cell types, based on gene expression. This was done using two patient cohorts, TCGA and BC Cancer Personalized OncoGenomics (POG). ResultsWe observed significant differences in immune cell composition between CRC and normal adjacent colon tissue, as well as differences in based on method of analysis. Evaluation of survival based on immune cell types revealed dendritic cells as a positive prognostic marker, consistently across methodologies. Mast cells were also found to be a positive prognostic marker, but in a stage-dependent manner. Unsupervised cluster analysis demonstrated that significant differences in immune cell composition has a more pronounced effect on prognosis in early-stage CRC, compared to late-stage CRC. This analysis revealed a distinct group of individuals with early-stage CRC which have an immune infiltration signature that indicates better survival probability. ConclusionsTaken together, characterization of the immune landscape in CRC has provided a powerful tool to assess prognosis. We anticipate that further characterization of the immune landscape will facilitate use of immunotherapies in CRC.
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