期刊
APPLIED SCIENCES-BASEL
卷 13, 期 7, 页码 -出版社
MDPI
DOI: 10.3390/app13074609
关键词
experimental design; HS-SPME; wine aroma; Saccharomyces cerevisiae; Hanseniaspora osmophila
For oenological products, the quality is determined by specific aromatic profiles, which are influenced by grape variety, geographical characteristics, and winemaking practices. A comprehensive analysis of flavour compounds using HS-SPME and GC-MS is necessary to evaluate the quality of wine. In this study, a method combining HS-SPME and GC-MS was developed to investigate the volatile compounds in wine samples. The experimental conditions of HS-SPME were optimized using a composite experimental design. The optimized method confirmed the effectiveness of combining selected yeast strains with grape inoculation to enhance the wine bouquet.
For oenological products, most of the intrinsic and extrinsic drivers of perceived quality are associated with specific aromatic profiles. Aromatic diversity has been recognized as a central element in perceived quality as it is able to transmit the complex interactions between grape variety, geographical characteristics, and viticultural and winemaking practices, including the fermentative process. A comprehensive characterization of flavour compounds by headspace solid-phase microextraction (HS-SPME) and gas chromatography coupled to mass spectrometric analysis is often needed in order to ascertain the quality of wine. HS-SPME requires a proper optimization that can be achieved through an adequate experimental design. Here, a HS-SPME/GC-MS based method was developed to investigate the volatile compounds of wine samples obtained by laboratory-scale fermentations. This was performed by inoculating a commercial Saccharomyces cerevisiae strain, which is used both as single starter and as mixed starter, with an indigenous Hanseniaspora osmophila strain. The experimental conditions of HS-SPME (extraction temperature and time) were optimized by applying a face-centred composite experimental design. Up to 95% of the total variance was explained by the proposed model. The optimized method allowed us to confirm the usefulness of combining the inoculation of grapes with selected yeast strains in co-culture situations in order to improve the wine bouquet.
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