期刊
CHEMCATCHEM
卷 7, 期 15, 页码 2313-2317出版社
WILEY-V C H VERLAG GMBH
DOI: 10.1002/cctc.201500218
关键词
aldehyde oxidase; amino alcohols; cascade reactions; enzyme catalysis; lactams
资金
- Innovative Medicines Initiative Joint Undertaking [115360]
- European Union
- EFPIA
- Science and Industry Endowment (SIEF)
- Engineering and Physical Sciences Research Council (EPSRC)
- Royal Society Wolfson Merit Award
The use of selected engineered galactose oxidase (GOase) variants for the oxidation of amino alcohols to aldehydes under mild conditions in aqueous systems is reported. GOase variant F-2 catalyses the regioselective oxidation of N-carbobenzyloxy (Cbz)-protected 3-amino-1,2-propanediol to the corresponding -hydroxyaldehyde which was then used in an aldolase reaction. Another variant, M3-5, was found to exhibit activity towards free and N-Cbz-protected aliphatic and aromatic amino alcohols allowing the synthesis of lactams such as 3,4-dihydronaphthalen-1(2H)-one, 2-pyrrolidone and valerolactam in one-pot tandem reactions with xanthine dehydrogenase (XDH) or aldehyde oxidase (PaoABC).
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