期刊
ADVANCED SCIENCE
卷 10, 期 11, 页码 -出版社
WILEY
DOI: 10.1002/advs.202203573
关键词
esophageal squamous cell carcinoma recurrence; myosin-9; sterile alpha motif domain-containing protein 9; beta-catenin signaling
In this study, the authors identified SAMD9 as a predictor and promoter of postoperative recurrence in ESCC. SAMD9 activates the Wnt/β-catenin signaling pathway, enhancing tumor stemness, angiogenesis, and EMT. Silencing SAMD9 or related genes MYH9 and β-catenin can inhibit multiple malignant properties of ESCC. SAMD9 has the potential to be a predictive biomarker and crucial therapeutic target for ESCC.
Recurrence is a challenge to survival after the initial treatment of esophageal squamous cell carcinoma (ESCC). But, its mechanism remains elusive and there are currently no biomarkers to predict postoperative recurrence. Here, the possibility of sterile alpha motif domain-containing protein 9 (SAMD9) as a predictor of postoperative recurrence of ESCC is evaluated and the molecular mechanisms by which SAMD9 promotes ESCC recurrence are elucidated. The authors found that the high level of SAMD9 is correlated with postoperative recurrence and poor prognosis of ESCC. Overexpression of SAMD9 promotes tumor stemness, angiogenesis, and EMT, while downregulation of SAMD9 reduced these phenotypes. Mechanistically, it is found that SAMD9 stimulated ubiquitination-mediated glycogen synthase kinase-3 beta (GSK-3 beta) degradation by interaction with myosin-9 (MYH9) and TNF receptor-associated factor 6 (TRAF6), which in turn activated Wnt/beta-catenin pathway. Further, the authors demonstrated that silencing SAMD9 inhibited lung metastasis and tumor formation in vivo. Finally, the authors found that silencing MYH9 or beta-catenin, or overexpressing GSK-3 beta inhibited SAMD9-stimulated ESCC cell stemness, EMT, angiogenesis, metastasis, and tumorigenicity. Together, the findings indicate that the SAMD9/MYH9/GSK3 beta/beta-catenin axis promotes ESCC postoperative recurrence and that SAMD9 is a crucial target for ESCC therapy. Additionally, SAMD9 has the potential as a predictor of postoperative recurrence in ESCC.
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