期刊
ACTA AMAZONICA
卷 53, 期 2, 页码 -出版社
INST NACIONAL PESQUISAS AMAZONIA
DOI: 10.1590/1809-4392202201741
关键词
zoonotic filariasis; non-human primates; Amazonia; emerging infectious disease
The primates in the rainforest of Manaus, Brazil are potential reservoirs of emerging and re-emerging infectious diseases. The use of PCR amplification of filarial sequences has shown that biting insects in the region have fed on non-human primates, indicating their potential role as disease vectors. However, the lack of primate-derived filarial parasite reference sequences limits the broader use of this method.
The primates that inhabit the rainforest surrounding the city of Manaus (Amazonas, Brazil) have long been recognised as potentially important reservoirs of emerging and re-emerging infectious diseases (ERIDs). PCR amplification of filarial sequences from wild-caught Simulium oyapockense has been used to incriminate potentially important Amazon-region ERID bridge vectors by showing they had previously fed on non-human primates. The broader use of filarial parasite sequences for the incrimination of biting insects as potentially important zoonotic disease vectors is limited by a paucity of primate-derived filarial parasite reference sequences which can be matched to the PCR amplified sequences obtained from insect-vector vectors. Here we have used shotgun sequencing to obtain reference data from an adult Dipetalonema gracile parasite which was found infecting a wild pied tamarin (Saguinus bicolor) in a peripheral region of Manaus. We report the parasite ' s complete mitochondrial genome (which is 13,647 base pairs in length), 894,846 base pairs of its Wolbachia genome and 6,426 base pairs of its ribosomal DNA locus (spanning from the start of its 18S subunit to the end of its 28S subunit). Despite being critically endangered, S. bicolor is commonly encountered around the periphery of Manaus and in urban forest fragments. The reported sequences may be a useful reference tool for identifying ERID bridge vectors and potentially provide some insights into the amount and the nature of contact between primate pathogen reservoirs and the residents of Manaus.
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