☆ 4.7 Article

Discovery of DNA aptamers targeting SARS-CoV-2 nucleocapsid protein and protein-binding epitopes for label-free COVID-19 diagnostics

MOLECULAR THERAPY-NUCLEIC ACIDS (2023)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Article Biophysics

Aptasensing nucleocapsid protein on nanodiamond assembled gold interdigitated electrodes for impedimetric SARS-CoV-2 infectious disease assessment

Santheraleka Ramanathan et al.

Summary: The strategy using a 10 μm gap-sized gold interdigitated electrode (AuIDE) with silane-modified diamond has shown enhanced detection of SARS-CoV-2 nucleocapsid protein (NCP). Structural analyses confirmed the cubic crystalline nature of the chemically modified diamond, with XPS analysis showing a strong interaction of carbon element. FTIR analysis revealed major intrinsic bondings between elements, making the aptasensing platform highly recommended for early diagnosis of SARS-CoV-2 infection.

BIOSENSORS & BIOELECTRONICS (2022)

添加到收藏夹

Article Chemistry, Analytical

Aptamer/antibody sandwich method for digital detection of SARS-CoV2 nucleocapsid protein

Chenchen Ge et al.

Summary: A microfluidic chip with femtoliter-sized wells was developed for sensitive digital detection of the N protein in SARS-CoV2. This method showed a detection limit of 33.28 pg/mL, which was 300 times lower than traditional ELISA, making it an accurate tool for early diagnosis of SARS-CoV2.

TALANTA (2022)

添加到收藏夹

Article Chemistry, Analytical

SARS-CoV-2 detection with aptamer-functionalized gold nanoparticles

Srivatsa Aithal et al.

Summary: The study introduces a rapid detection test for SARS-CoV-2 utilizing nanoprobes functionalized with specific aptamer to identify spike membrane protein. The nanoprobes successfully detect spike protein concentrations of 16 nM and higher.

TALANTA (2022)

添加到收藏夹

Article Multidisciplinary Sciences

Heat inactivation of clinical COVID-19 samples on an industrial scale for low risk and efficient high-throughput qRT-PCR diagnostic testing

Oona Delpuech et al.

Summary: This study presents a large-scale heat inactivation process for clinical COVID-19 samples prior to laboratory processing. The modified process utilizes industrial catering ovens for bulk heat inactivation, allowing subsequent activities to be performed in open laboratories. The method proves to be cost-effective, improves operator safety and ergonomics, and is scalable. It is especially suitable for streamlined workflows like 'direct to PCR' assays.

SCIENTIFIC REPORTS (2022)

添加到收藏夹

Article Biochemistry & Molecular Biology

Search and sequence analysis tools services from EMBL-EBI in 2022

Fabio Madeira et al.

Summary: The EMBL-EBI search and sequence analysis tools frameworks allow integrated access to data resources and core bioinformatics analytical tools. These tools can be interacted with through user-friendly web applications and APIs. Recent improvements have been made to accommodate the increasing data requirements during the COVID-19 pandemic.

NUCLEIC ACIDS RESEARCH (2022)

添加到收藏夹

Review Cell Biology

Monoclonal antibodies for COVID-19 therapy and SARS-CoV-2 detection

Yu-Chyi Hwang et al.

Summary: The COVID-19 pandemic has created a public health crisis, leading to the urgent development of therapeutic treatments and virus detection methods. Monoclonal antibodies (mAbs) have emerged as powerful tools for treating and detecting diseases due to their high specificity and reliability. Researchers are urgently developing antibody-based kits for SARS-CoV-2 detection and antibody drugs for COVID-19 treatment. The spike protein of SARS-CoV-2, which is crucial for viral infection, has been extensively studied and its receptor-binding domain (RBD) has become a major target for therapeutic antibody development. Given the high mutation rate of SARS-CoV-2, especially under the pressure of prophylactic vaccines and neutralizing antibodies, the use of antibody cocktails is expected to be an important strategy for effective COVID-19 treatment. Additionally, antibodies against cytokine storms, which can be triggered by SARS-CoV-2 infection and drive severe disease progression, are also being developed as treatments for COVID-19. In addition to their use as drugs, antibodies are currently being used in SARS-CoV-2 detection tests, including antigen and immunoglobulin tests, which are crucial surveillance tools for preventing the spread of COVID-19.

JOURNAL OF BIOMEDICAL SCIENCE (2022)

添加到收藏夹

Review Chemistry, Multidisciplinary

Nucleic Acid Aptamers for Molecular Diagnostics and Therapeutics: Advances and Perspectives

Long Li et al.

Summary: SELEX technology has shown the ability to evolve artificial ligands with affinity and specificity, and aptamers complement antibodies with unique advantages such as small size, low cost, and facile chemical modification, making them a potential molecular tool for future clinical needs in biomedicine.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

添加到收藏夹

Article Biophysics

COVID-19 diagnosis - A review of current methods

Meral Yuce et al.

Summary: A fast and accurate self-testing tool for COVID-19 diagnosis is crucial for understanding the global situation and taking appropriate actions. The diagnosis tools available mainly rely on viral gene detection, human antibody detection, and viral antigen detection, with RT-PCR being the most reliable technique.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Infectious Diseases

Accuracy of a nucleocapsid protein antigen rapid test in the diagnosis of SARS-CoV-2 infection

Bo Diao et al.

Summary: The FIC assay for NP antigen testing shows high specificity and relatively high sensitivity in early-phase SARS-CoV-2 diagnosis, according to a study conducted in Wuhan and Chongqing between February 10 and 15, 2020.

CLINICAL MICROBIOLOGY AND INFECTION (2021)

添加到收藏夹

Review Virology

The Main Molecular and Serological Methods for Diagnosing COVID-19: An Overview Based on the Literature

Bruna Aparecida Souza Machado et al.

Summary: Diagnostic tests are crucial for controlling COVID-19, with nucleic acid-based tests for initial virus detection and serological tests for disease progression assessment. Test performance varies based on factors like sample type and assay characteristics, with positivity linked to symptom onset. Point-of-care testing is a cost-effective trend, but analytical performance must be carefully evaluated.

VIRUSES-BASEL (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Neutralizing Aptamers Block S/RBD-ACE2 Interactions and Prevent Host Cell Infection

Xiaohui Liu et al.

Summary: The receptor-binding domain of the SARS-CoV-2 spike protein is crucial for mediating virus entry into human cells, making it an ideal target for blocking infection. Using aptamers specifically targeting the S/RBD, researchers were able to effectively prevent virus binding to host cells and neutralize viral particles expressing the S protein, demonstrating a promising COVID-19 therapeutic strategy.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

A SARS-CoV-2 Spike Binding DNA Aptamer that Inhibits Pseudovirus Infection by an RBD-Independent Mechanism**

Anton Schmitz et al.

Summary: A specific aptamer that interacts with the coronavirus SARS-CoV-2 has been identified, showing potent and specific inhibition of pseudoviral infection despite not blocking the virus's interaction with ACE2. This study opens new avenues for developing inhibitors of SARS-CoV-2 infection and highlights the potential of aptamers as drug candidates and tools for unraveling previously inaccessible infection modalities.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

添加到收藏夹

Article Biophysics

Paper-based electrochemical biosensor for diagnosing COVID-19: Detection of SARS-CoV-2 antibodies and antigen

Abdulhadee Yakoh et al.

Summary: A specific and sensitive immunosensor has been developed for the detection of immunoglobulins produced against SARS-CoV-2, targeting SARS-CoV-2 antibodies without the need for specific antibodies. This sensor has shown effective results in real clinical sera and has the potential for expanded use in antigen detection, presenting new possibilities for diagnosing COVID-19.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Biophysics

Hitting the diagnostic sweet spot: Point-of-care SARS-CoV-2 salivary antigen testing with an off-the-shelf glucometer

Naveen K. Singh et al.

Summary: The study introduces an aptamer-based SARS-CoV-2 salivary antigen assay that utilizes low-cost reagents and an off-the-shelf glucometer. This test is highly scalable, provides rapid results within 1 hour, and can accurately detect viral loads in infected specimens while distinguishing them from uninfected controls.

BIOSENSORS & BIOELECTRONICS (2021)

添加到收藏夹

Article Chemistry, Analytical

Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR

Lianhua Dong et al.

Summary: The COVID-19 outbreak caused by a novel Coronavirus has spread globally. Current gold standard RT-qPCR for SARS-CoV-2 diagnosis shows varying sensitivity, highlighting the need for more accurate methods. A study on RT-dPCR demonstrated significant improvement in detection sensitivity, particularly for asymptomatic patients, making it a highly accurate method for screening suspected cases.

TALANTA (2021)

添加到收藏夹

Review Chemistry, Multidisciplinary

COVID-19 Antibody Tests and Their Limitations

Guoqiang Liu et al.

Summary: COVID-19 pandemic is a global health crisis with over 2 million deaths, where the main diagnostic methods are nucleic acid PCR tests, viral antigen tests, and human antibody tests. The limitations of antibody tests include inaccuracies due to various factors such as testing windows, individual variances, and fluctuation in antibody levels.

ACS SENSORS (2021)

添加到收藏夹

Article Microbiology

Identification and functional analysis of the SARS-COV-2 nucleocapsid protein

Tianyi Gao et al.

Summary: This study analyzed the physicochemical properties, subcellular localization, and biological function of the SARS-COV-2N protein. The identification of 12 phosphorylated sites and 9 potential protein kinase sites in the SARS-COV-2N protein may serve as promising targets for drug discovery and development of a recombinant virus vaccine.

BMC MICROBIOLOGY (2021)

添加到收藏夹

Article Medicine, Research & Experimental

A novel G-quadruplex aptamer-based spike trimeric antigen test for the detection of SARS-CoV-2

Ankit Gupta et al.

Summary: The study identified a DNA aptamer with strong binding ability to the spike trimer antigen of SARS-CoV-2 using Systematic Evolution of Ligands by EXponential enrichment. The aptamer, named S14, showed high affinity and structure, capable of detecting antigens as low as 2 nM. Clinical evaluation demonstrated its highly discriminatory performance in differentiating between SARS-CoV-2 infected and non-infected individuals.

MOLECULAR THERAPY-NUCLEIC ACIDS (2021)

添加到收藏夹

Article Chemistry, Analytical

SARS-CoV-2 spike protein detection through a plasmonic D-shaped plastic optical fiber aptasensor

Nunzio Cennamo et al.

Summary: A specific aptameric sequence has been immobilized on a gold nano-film deposited on a D-shaped plastic optical fiber, allowing the monitoring of protein binding through the surface plasmon resonance phenomenon. The aptasensor specifically developed using the receptor-binding domain of the SARS-CoV-2 spike glycoprotein showed a high sensitivity in detecting the spike protein in a range of 25 to 1000 nM, with a Limit of Detection of about 37 nM. Various interferents were tested to confirm the specificity of the aptasensor, indicating its potential application as a point-of-care device.

TALANTA (2021)

添加到收藏夹

Article Chemistry, Analytical

Surface plasmon resonance aptasensor based on niobium carbide MXene quantum dots for nucleocapsid of SARS-CoV-2 detection

Rongyuan Chen et al.

Summary: A novel label-free SPR aptasensor using thiol-modified niobium carbide MXene quantum dots was constructed for detecting N-gene of SARS-CoV-2. The sensor exhibited a low limit of detection and high selectivity, providing an effective qualitative analysis of the N-gene in complex environments.

MICROCHIMICA ACTA (2021)

添加到收藏夹

Review Microbiology

Structural biology of SARS-CoV-2 and implications for therapeutic development

Haitao Yang et al.

Summary: The COVID-19 pandemic caused by SARS-CoV-2 has led to an unprecedented global health crisis, with limited therapeutic options available. Understanding the structure and function of the 29 proteins encoded by SARS-CoV-2 is crucial for developing novel therapeutics and treatment interventions. These proteins play various roles in the viral life cycle, from entry into host cells to genome replication and transcription, highlighting their potential as targets for therapeutic interventions.

NATURE REVIEWS MICROBIOLOGY (2021)

添加到收藏夹

Article Medicine, Research & Experimental

The role of SAXS and molecular simulations in 3D structure elucidation of a DNA aptamer against lung cancer

Dmitry Morozov et al.

Summary: Aptamers, short DNA or RNA molecules, bind to target molecules with specificity, with affinity depending on their tertiary structure and charge distribution. Optimizing length and structure is crucial for improving aptamer specificity and affinity. LC-18t, a truncated version of aptamer LC-18, showed stronger binding to cancerous cells in lung tumor tissues and shared the binding site with the original aptamer.

MOLECULAR THERAPY-NUCLEIC ACIDS (2021)

添加到收藏夹

Article Multidisciplinary Sciences

Characterization of SARS-CoV-2 nucleocapsid protein reveals multiple functional consequences of the C-terminal domain

Chao Wu et al.

Summary: The study characterizes the biochemical properties of the nucleocapsid protein N encoded by SARS-CoV-2 and highlights the value of using N domains as highly specific and sensitive diagnostic markers. The research provides insights into N oligomerization, RNA binding, phosphorylation regulation, and immunogenicity of different N domains.

ISCIENCE (2021)

添加到收藏夹

Article Chemistry, Analytical

Localized surface plasmon resonance aptasensor for selective detection of SARS-CoV-2 S1 protein

Tyra Lewis et al.

Summary: This study developed a novel method for precise detection of the S1 spike protein of SARS-CoV-2, showing high selectivity and specificity with excellent performance in terms of dynamic range and limit of detection. Utilizing aptamer technology, the method demonstrated good stability and repeatability in real-time monitoring.

ANALYST (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Mix-and-read, one-minute SARS-CoV-2 diagnostic assay: development of PIFE-based aptasensor

J. Michelle Lee et al.

Summary: The developed SARS-CoV-2 antigen assay based on protein-induced fluorescence enhancement of a DNA aptamer shows significant selectivity and sensitivity towards the virus, with improvements in speed and manufacturability. The platform is proposed as a viable testing strategy for clinical settings, especially in low- and middle-income countries.

CHEMICAL COMMUNICATIONS (2021)

添加到收藏夹

Article Medicine, General & Internal

Genomic characterisation and epidemiology of 2019 novel coronavirus: implications for virus origins and receptor binding

Roujian Lu et al.

LANCET (2020)

添加到收藏夹

Editorial Material Biotechnology & Applied Microbiology

Therapeutic options for the 2019 novel coronavirus (2019-nCoV)

Guangdi Li et al.

NATURE REVIEWS DRUG DISCOVERY (2020)

添加到收藏夹

Review Chemistry, Multidisciplinary

Diagnosing COVID-19: The Disease and Tools for Detection

Buddhisha Udugama et al.

ACS NANO (2020)

添加到收藏夹

Article Chemistry, Analytical

Rapid and Sensitive Detection of anti-SARS-CoV-2 IgG, Using Lanthanide-Doped Nanoparticles-Based Lateral Flow Immunoassay

Zhenhua Chen et al.

ANALYTICAL CHEMISTRY (2020)

添加到收藏夹

Article Biochemical Research Methods

The HDOCK server for integrated protein-protein docking

Yumeng Yan et al.

NATURE PROTOCOLS (2020)

添加到收藏夹

Letter Virology

A DNA Aptamer Based Method for Detection of SARS-CoV-2 Nucleocapsid Protein

Zhiqiang Chen et al.

VIROLOGICA SINICA (2020)

添加到收藏夹

Article Chemistry, Analytical

Discovery of Aptamers Targeting the Receptor-Binding Domain of the SARS-CoV-2 Spike Glycoprotein

Yanling Song et al.

ANALYTICAL CHEMISTRY (2020)

添加到收藏夹

Article Microbiology

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT-qPCR primer-probe sets

Chantal B. F. Vogels et al.

NATURE MICROBIOLOGY (2020)

添加到收藏夹

Article Biochemistry & Molecular Biology

Bio-Layer Interferometry Analysis of the Target Binding Activity of CRISPR-Cas Effector Complexes

Hanna Mueller-Esparza et al.

FRONTIERS IN MOLECULAR BIOSCIENCES (2020)

添加到收藏夹

Article Immunology

Methods of Inactivation of SARS-CoV-2 for Downstream Biological Assays

Edward Patterson et al.

JOURNAL OF INFECTIOUS DISEASES (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-PCR

Ioanna Smyrlaki et al.

NATURE COMMUNICATIONS (2020)

添加到收藏夹

Article Multidisciplinary Sciences

Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

Adriana Savastano et al.

NATURE COMMUNICATIONS (2020)

添加到收藏夹

Article Engineering, Biomedical

Rapid lateral flow immunoassay for the fluorescence detection of SARS-CoV-2 RNA

Daming Wang et al.

NATURE BIOMEDICAL ENGINEERING (2020)

添加到收藏夹

Article Chemistry, Multidisciplinary

Discovery of sandwich type COVID-19 nucleocapsid protein DNA aptamers

Liyun Zhang et al.

CHEMICAL COMMUNICATIONS (2020)

添加到收藏夹

Review Microbiology

Application of Aptamers in Virus Detection and Antiviral Therapy

Xinran Zou et al.

FRONTIERS IN MICROBIOLOGY (2019)

添加到收藏夹

Article Chemistry, Analytical

Systematic truncating of aptamers to create high-performance graphene oxide (GO)-based aptasensors for the multiplex detection of mycotoxins

Xinglin Wang et al.

ANALYST (2019)

添加到收藏夹

Article Multidisciplinary Sciences

Rational truncation of aptamer for cross-species application to detect krait envenomation

Abhijeet Dhiman et al.

SCIENTIFIC REPORTS (2018)

添加到收藏夹

Article Multidisciplinary Sciences

Three-dimensional modeling of single stranded DNA hairpins for aptamer-based biosensors

Iman Jeddi et al.

SCIENTIFIC REPORTS (2017)

添加到收藏夹

Review Biochemical Research Methods

Therapeutic applications of synthetic nucleic acid aptamers

Suttinee Poolsup et al.

CURRENT OPINION IN BIOTECHNOLOGY (2017)

添加到收藏夹

Article Biochemistry & Molecular Biology

SimRNA: a coarse-grained method for RNA folding simulations and 3D structure prediction

Michal J. Boniecki et al.

NUCLEIC ACIDS RESEARCH (2016)

添加到收藏夹

Review Chemistry, Multidisciplinary

Small-Molecule Binding Aptamers: Selection Strategies, Characterization, and Applications

Annemaria Ruscito et al.

FRONTIERS IN CHEMISTRY (2016)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Hi-Fi SELEX: A high-fidelity digital-PCR based therapeutic aptamer discovery platform

Eric Ouellet et al.

BIOTECHNOLOGY AND BIOENGINEERING (2015)

添加到收藏夹

Article Chemistry, Physical

ff14SB: Improving the Accuracy of Protein Side Chain and Backbone Parameters from ff99SB

James A. Maier et al.

JOURNAL OF CHEMICAL THEORY AND COMPUTATION (2015)

添加到收藏夹

Letter Biochemical Research Methods

The I-TASSER Suite: protein structure and function prediction

Jianyi Yang et al.

NATURE METHODS (2015)

添加到收藏夹

Article Medicine, Research & Experimental

FASTAptamer: A Bioinformatic Toolkit for High-throughput Sequence Analysis of Combinatorial Selections

Khalid K. Alam et al.

MOLECULAR THERAPY-NUCLEIC ACIDS (2015)

添加到收藏夹

Article Biochemical Research Methods

Accurate Proteome-wide Label-free Quantification by Delayed Normalization and Maximal Peptide Ratio Extraction, Termed MaxLFQ

Juergen Cox et al.

MOLECULAR & CELLULAR PROTEOMICS (2014)

添加到收藏夹

Article Biochemical Research Methods

System-wide Perturbation Analysis with Nearly Complete Coverage of the Yeast Proteome by Single-shot Ultra HPLC Runs on a Bench Top Orbitrap

Nagarjuna Nagaraj et al.

MOLECULAR & CELLULAR PROTEOMICS (2012)

添加到收藏夹

Article Biotechnology & Applied Microbiology

Novel system for detecting SARS coronavirus nucleocapsid protein using an ssDNA aptamer

Seong-Je Cho et al.

JOURNAL OF BIOSCIENCE AND BIOENGINEERING (2011)

添加到收藏夹

Article Biochemical Research Methods

Andromeda: A Peptide Search Engine Integrated into the MaxQuant Environment

Juergen Cox et al.

JOURNAL OF PROTEOME RESEARCH (2011)

添加到收藏夹

Article Biochemical Research Methods

RNAstructure: software for RNA secondary structure prediction and analysis

Jessica S. Reuter et al.

BMC BIOINFORMATICS (2010)

添加到收藏夹

Article Chemistry, Multidisciplinary

Aptamer-facilitated biomarker discovery (AptaBiD)

Maxim V. Berezovski et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2008)

添加到收藏夹

Article Biotechnology & Applied Microbiology

MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification

Juergen Cox et al.

NATURE BIOTECHNOLOGY (2008)

添加到收藏夹

Article Biochemistry & Molecular Biology

IDT SciTools: a suite for analysis and design of nucleic acid oligomers

Richard Owczarzy et al.

NUCLEIC ACIDS RESEARCH (2008)

添加到收藏夹

Review Pathology

Single-molecule PCR: an artifact-free PCR approach for the analysis of somatic mutations

Y Kraytsberg et al.

EXPERT REVIEW OF MOLECULAR DIAGNOSTICS (2005)

添加到收藏夹

Article Immunology

Nucleocapsid protein as early diagnostic marker for SARS

XY Che et al.

EMERGING INFECTIOUS DISEASES (2004)

添加到收藏夹

Article Biochemistry & Molecular Biology

Mfold web server for nucleic acid folding and hybridization prediction

M Zuker

NUCLEIC ACIDS RESEARCH (2003)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.