Sanghuangporus sanghuang extract extended the lifespan and healthspan of Caenorhabditis elegans via DAF-16/SIR-2.1

Sanghuangporus Sanghuang is a fungus species. As a traditional Chinese medicine, it is known for antitumor, antioxidant and anti-inflammatory properties. However, the antiaging effect of S. Sanghuang has not been deeply studied. In this study, the effects of S. Sanghuang extract (SSE) supernatants on the changes of nematode indicators were investigated. The results showed that different concentrations of SSE prolonged the lifespans of nematodes and substantially increased these by 26.41%. In addition, accumulations of lipofuscin were also visibly reduced. The treatment using SSE also played a role in increasing stress resistance, decreasing ROS accumulations and obesity, and enhancing the physique. RT-PCR analysis showed that the SSE treatment upregulated the transcription of daf-16, sir-2.1, daf-2, sod-3 and hsp-16.2, increased the expression of these genes in the insulin/IGF-1 signalling pathway and prolonged the lifespans of nematodes. This study reveals the new role of S. Sanghuang in promoting longevity and inhibiting stress and provides a theoretical basis for the application of S. Sanghuang in anti-ageing treatments.

Automated summary

This study found that the extract of S. Sanghuang (SSE) has positive effects on nematode indicators. Different concentrations of SSE can prolong the lifespan of nematodes and increase it by 26.41%. SSE also reduces the accumulation of endogenous aging substances, increases antioxidant capacity and enhances physical fitness. RT-PCR analysis shows that SSE treatment upregulates the expression of a series of genes, prolonging the lifespan of nematodes. This study reveals the new role of S. Sanghuang in promoting longevity and inhibiting stress, providing a theoretical basis for its application in anti-aging treatments.