期刊
CATALYSTS
卷 13, 期 5, 页码 -出版社
MDPI
DOI: 10.3390/catal13050790
关键词
gold nanoparticles; antibody/antigen ligands; protein-ligand interaction; biofunctionalization; biomolecules; Neisseria meningitides
This study developed a homemade Neisseria meningitidis recombinant antibody (scFv-Fc) by connecting the fragment crystallizable (Fc) region of a human antibody with a mouse recombinant antibody (single-chain variable fragment antibody, scFv), and characterized it using the SOEing PCR technique. An optimized gold coating agent for the scFv-Fc/Au NP conjugation (citrate agent) was found among three common agents (citrate, allylamine hydrochloride, and polyvinyl alcohol) with different surface charges. The stability of the scFv-Fc/protein A-G in the presence of a N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) linker was also investigated using the docking method.
Recent advances in the use of gold nanoparticles (Au NPs)/antibody conjugations in nanomedicine have increased the need to optimize the synthesis conditions and surface functionalization of Au NPs. In this study, a home-made Neisseria meningitidis recombinant antibody (scFv-Fc) was developed by connecting the fragment crystallizable (Fc) region of a human antibody with a mouse recombinant antibody (single-chain variable fragment antibody (scFv)) and characterized using the SOEing PCR technique. Then, an optimized gold coating agent for the scFv-Fc/Au NP conjugation (i.e., the citrate agent) was found among three common agents (citrate, allylamine hydrochloride, and polyvinyl alcohol) with different surface charges (negative, positive, and neutral, respectively). Moreover, the stability of the scFv-Fc/protein A-G in the presence of a N-succinimidyl 3-(2-pyridyldithio) propionate (SPDP) linker was investigated using the docking method. It was found that the designed scFv-Fc/protein A-G/SPDP/citrate recombinant antibody showed optimized bottom-on conjugation of the protein A-G with the improved scFv-Fc/Au NPs, enabling a suitable interaction with the Neisseria meningitidis bacterial antigen.
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