Lipase B from Candida antarctica in Highly Saline AOT-Water-Isooctane Reverse Micelle Systems for Enhanced Esterification Reaction

Butyl oleate synthesis by the lipase B from Candida antarctica (CalB) under extreme halophilic conditions was investigated in the present research through the AOT/Water/Isooctane reverse micellar system. The impact of aqueous content (W-o(=) [H2O]/[Surfactant] and NaCl variation on the enzymatic activity of CalB in the butyl oleate reaction in reverse micelles was explored. The results indicated that, based on the increase of NaCl, it is remarkable to achieve higher enzymatic activity up to 444.85 mu mol/min at 5 M NaCl and Wo = 10, as the best esterification conditions at pH 7.2 and 30 degrees C. However, it was clear that butyl oleate synthesis by lipase CalB increased based on the reduction in the average reverse micelle size, where reverse micelle sizes were determined by dynamic light scattering (DLS). This increase in butyl oleate synthesis demonstrated the potential of reverse micelles as systems that enhance mass transport phenomena in heterogeneous biocatalysis. Furthermore, reverse micelles are promising systems for extreme halophilic lipases research.

Automated summary

The synthesis of butyl oleate by the lipase B from Candida antarctica (CalB) was studied in a reverse micellar system under extreme halophilic conditions. The impact of aqueous content and NaCl variation on the enzymatic activity of CalB in the butyl oleate reaction in reverse micelles was investigated. The results showed that increasing NaCl concentration led to higher enzymatic activity, reaching a maximum of 444.85 μmol/min at 5 M NaCl and Wo = 10, with pH 7.2 and 30 degrees C being the optimal conditions. However, the synthesis of butyl oleate by lipase CalB increased due to the reduction in the average reverse micelle size, as determined by dynamic light scattering (DLS). This study demonstrated the potential of reverse micelles as a system to enhance mass transport in heterogeneous biocatalysis and highlighted their promise for extreme halophilic lipases research.