期刊
BIOLOGY OPEN
卷 12, 期 7, 页码 -出版社
COMPANY BIOLOGISTS LTD
DOI: 10.1242/bio.059976
关键词
Cilia; Dynein-2; IFT-B complex; Intraflagellar transport
类别
The dynein-2 complex within cilia needs to be transported as an anterograde cargo to enable its function in retrograde trafficking. Previous research has shown that interaction of multiple IFT-B subunits, including IFT54, with WDR60 and the DYNC2H1-DYNC2LI1 dimer of dynein-2 is necessary for the trafficking of dynein-2. However, deleting the IFT54-binding site from WDR60 only has a minor effect on dynein-2 trafficking and function. The results suggest that the C-terminal coiled-coil region of IFT54 is essential for IFT-B function, and the middle linker region of IFT54 is required for ciliary retrograde trafficking by mediating the binding of IFT-B to the dynein-2 complex.
Within cilia, the dynein-2 complex needs to be transported as an anterograde cargo to achieve its role as a motor to drive retrograde trafficking of the intraflagellar transport (IFT) machinery containing IFT-A and IFT-B complexes. We previously showed that interactions of WDR60 and the DYNC2H1-DYNC2LI1 dimer of dynein-2 with multiple IFT-B subunits, including IFT54, are required for the trafficking of dynein-2 as an IFT cargo. However, specific deletion of the IFT54-binding site from WDR60 demonstrated only a minor effect on dynein-2 trafficking and function. We here show that the C-terminal coiled-coil region of IFT54, which participates in its interaction with the DYNC2H1-DYNC2LI1 dimer of dynein-2 and with IFT20 of the IFT-B complex, is essential for IFT-B function, and suggest that the IFT54 middle linker region between the N-terminal WDR60-binding region and the C-terminal coiled-coil is required for ciliary retrograde trafficking, probably by mediating the effective binding of IFT-B to the dynein-2 complex, and thereby ensuring dynein-2 loading onto the anterograde IFT trains. The results presented here agree with the notion predicted from the previous structural models that the dynein-2 loading onto the anterograde IFT train relies on intricate, multivalent interactions between the dynein-2 and IFT-B complexes.
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