Acclimation and hardening of a slow-growing woody species emblematic to western North America from in vitro plantlets

PremiseDetermining the tolerance of plant populations to climate change requires the development of biotechnological protocols producing genetically identical individuals used for genotype-by-environment experiments. Such protocols are missing for slow-growth, woody plants; to address this gap, this study uses Artemisia tridentata, a western North American keystone shrub, as model. Methods and ResultsThe production of individual lines is a two-step process: in vitro propagation under aseptic conditions followed by ex vitro acclimation and hardening. Due to aseptic growth conditions, in vitro plantlets exhibit maladapted phenotypes, and this protocol focuses on presenting an approach promoting morphogenesis for slow-growth, woody species. Survival was used as the main criterion determining successful acclimation and hardening. Phenotypic changes were confirmed by inspecting leaf anatomy, and shoot water potential was used to ensure that plantlets were not water stressed. ConclusionsAlthough our protocol has lower survival rates (11-41%) compared to protocols developed for herbaceous, fast-growing species, it provides a benchmark for slow-growth, woody species occurring in dry ecosystems.

Automated summary

This study develops a biotechnological protocol for producing genetically identical individuals to determine plant populations' tolerance to climate change. The protocol focuses on slow-growth, woody plants, using Artemisia tridentata as a model. The two-step process includes in vitro propagation and ex vitro acclimation and hardening, providing a benchmark for slow-growth, woody species occurring in dry ecosystems.