Insight into the biochemical and physiological mechanisms of nanoparticles-induced arsenic tolerance in bamboo

Introduction: Arsenic (As) contamination in soil, sediments, and water poses a significant threat to the growth of bamboo plants. However, nanoparticles with high metal absorbance capacity can play a key role in the reduction of heavy metals toxicity in plants as well as maintaining their growth under toxicity. Methods: Hence, an in vitro experiment was conducted to determine the influence of three types of nanoparticles: 150 mu M silicon nanoparticles (SiO2 NPs), 150 mu M titanium nanoparticles (TiO2 NPs), and 150 mu M zinc oxide nanoparticles (ZnO NPs) on As (150 mu M and 250 mu M) tolerance enhancement of a one-year-old bamboo species (Pleioblastus pygmaeus). Results and discussion: The results showed that while As at 150 mu M and 250 mu M significantly disrupted the plant growth by excessive generation of reactive oxygen species (ROS) components, and inducing cell membrane peroxidation, the addition of NPs increased both enzymatic and non-enzymatic antioxidant activities, upregulated glyoxalase defense system, and improved gas exchange parameters and photosynthetic pigments content, leading to the enhanced plant shoot and root dry weight. These were achieved by lowering levels of ROS, electrolyte leakage (EL), malondialdehyde (MDA), hydrogen peroxide (H2O2) and the superoxide radical (O-2(-center dot)), as well as decreasing As accumulation in the plant organs. Thus, it might be concluded that ZnO NPs, SiO(2)NPs, and TiO2NPS alone or in combination can significantly increase the bamboo plant tolerance to As toxicity via key mechanisms, including induction of various antioxidants and glyoxalase defense systems, scavenging of ROS and methylglyoxal (MG), increasing phytochelatins production, reduction of As accumulation and translocation, and improving photosynthetic pigments under As toxicity. Additionally, the results showed that the combined application of 150 mu M ZnO NPs, SiO2 NPs, and TiO2 NPs had the greatest effect on enhancing the plant tolerance to As at 150 mu M and 250 mu M.

Automated summary

ZnO NPs, SiO2 NPs, and TiO2 NPs can enhance the tolerance of bamboo plants to arsenic contamination by inducing various antioxidants and glyoxalase defense systems, scavenging reactive oxygen species and methylglyoxal, increasing phytochelatins production, reducing arsenic accumulation and translocation, and improving photosynthetic pigments content.