Multi-walled carbon nanotubes promote the accumulation, distribution, and assimilation of 15N-KNO3 in Malus hupehensis by entering the roots

IntroductionMulti-walled nanotubes (MWCNTs) consist of multiple rolled layers of graphene. Nitrogen plays an important role in apple growth. The effect of MWCNTs on nitrogen utilization in apple needs to be further investigated. MethodsIn this study, the woody plant Malus hupehensis seedlings were used as plant materials, the distribution of MWCNTs in the roots was observed, and the effects of MWCNTs on the accumulation, distribution, and assimilation of nitrate by the seedlings were explored. ResultsThe results showed that MWCNTs could penetrate the roots of Malus hupehensis seedlings, and the 50, 100, and 200 mu g center dot mL(-1) MWCNTs significantly promoted the root growth of seedlings, increased root number, root activity, fresh weight, and nitrate content of seedlings, and also increased nitrate reductase activity, free amino acid, and soluble protein content of roots and leaves. N-15 tracer experiments indicated that MWCNTs decreased the distribution ratio of N-15-KNO3 in Malus hupehensis roots but increased its distribution ratio in stems and leaves. MWCNTs improved the utilization ratio of N-15-KNO3 in Malus hupehensis seedlings, with the values being increased by 16.19%, 53.04%, and 86.44% following the 50, 100, and 200 mu g center dot mL(-1) MWCNTs, respectively. The RT-qPCR analysis showed that MWCNTs significantly affected the expression of genes (MhNRTs) related to nitrate uptake and transport in roots and leaves, and MhNRT1.4, MhNRT1.7, MhNRT1.8, MhNRT2.1, MhNRT2.5, and MhNRT2.7 were notably up-regulated in response to 200 mu g center dot mL(-1) MWCNTs. Raman analysis and transmission electron microscopy images indicated that MWCNTs could enter the root tissue of Malus hupehensis and were distributed between the cell wall and cytoplasmic membrane. Pearson correlation analysis showed that root tip number, root fractal dimension, and root activity were the main factors affecting root uptake and assimilation of nitrate. ConclusionsThese findings suggest that MWCNTs promoted root growth by entering the root, stimulated the expression of MhNRTs, and increased NR activity, thereby enhancing the uptake, distribution, and assimilation of nitrate by root, and ultimately improved the utilization of N-15-KNO3 by Malus hupehensis seedlings.

Automated summary

This study found that multi-walled nanotubes (MWCNTs) could enter the root system of apple and promote root growth, increase root number and activity, and enhance the absorption and utilization efficiency of nitrate by the plant. Meanwhile, MWCNTs can also regulate the expression of related genes, enhance nitrate reductase activity, and improve the distribution and assimilation of nitrate in the apple root system. These findings are of great significance for improving the nitrogen utilization efficiency of fruit trees.