Mycobacterial IHF is a highly dynamic nucleoid-associated protein that assists HupB in organizing chromatin

Nucleoid-associated proteins (NAPs) crucially contribute to organizing bacterial chromatin and regulating gene expression. Among the most highly expressed NAPs are the HU and integration host factor (IHF) proteins, whose functional homologues, HupB and mycobacterial integration host factor (mIHF), are found in mycobacteria. Despite their importance for the pathogenicity and/or survival of tubercle bacilli, the role of these proteins in mycobacterial chromosome organization remains unknown. Here, we used various approaches, including super-resolution microscopy, to perform a comprehensive analysis of the roles of HupB and mIHF in chromosome organization. We report that HupB is a structural agent that maintains chromosome integrity on a local scale, and that the lack of this protein alters chromosome morphology. In contrast, mIHF is a highly dynamic protein that binds DNA only transiently, exhibits susceptibility to the chromosomal DNA topology changes and whose depletion leads to the growth arrest of tubercle bacilli. Additionally, we have shown that depletion of Mycobacterium smegmatis integration host factor (msIHF) leads to chromosome shrinkage and replication inhibition.

Automated summary

Nucleoid-associated proteins (NAPs) play a crucial role in organizing bacterial chromatin and regulating gene expression. HupB and mycobacterial integration host factor (mIHF) are highly expressed NAPs in mycobacteria, but their role in mycobacterial chromosome organization is unknown. This study used various approaches to analyze the roles of HupB and mIHF in chromosome organization and found that HupB maintains chromosome integrity while mIHF binds DNA transiently and is susceptible to chromosomal DNA topology changes.