Rosette formation by Plasmodium vivax gametocytes favors the infection in Anopheles aquasalis

Plasmodium vivax is a public health problem and the most common type of malaria outside sub-Saharan Africa. The capacity of cytoadhesion, rosetting, and liver latent phase development could impact treatment and disease control. Although the ability to P. vivax gametocyte develop rosetting is known, it is not yet clear which role it plays during the infection and transmission process to the mosquito. Here, we used ex vivo approaches for evaluate the rosetting P. vivax gametocytes capacity and we have investigated the effect of this adhesive phenotype on the infection process in the vector Anopheles aquasalis mosquito. Rosette assays were performed in 107 isolates, and we have observed an elevated frequency of cytoadhesive phenomena (77,6%). The isolates with more than 10% of rosettes have presented a higher infection rate in Anopheles aquasalis (p=0.0252). Moreover, we found a positive correlation between the frequency of parasites in rosetting with the infection rate (p=0.0017) and intensity (p=0.0387) in the mosquito. The disruption of P. vivax rosette formation through mechanical rupture assay confirmed the previously findings, since the paired comparison showed that isolates with disrupted rosettes have a lower infection rate (p<0.0001) and intensity (p=0.0003) compared to the control group (no disruption). Herein we have demonstrated for the first time a potential effect of the rosette phenomenon on the infection process in the mosquito vector An. aquasalis, favoring its capacity and intensity of infection, thus allowing the perpetuation of the parasite cycle life.

Automated summary

Plasmodium vivax is the most common type of malaria outside sub-Saharan Africa, and its ability to adhere to cells, form rosettes, and develop in the liver can impact treatment and disease control. In this study, we used ex vivo methods to evaluate the rosetting capacity of P. vivax gametocytes and investigated its effect on infection in the mosquito vector Anopheles aquasalis. We found a high frequency of cytoadhesive phenomena and a positive correlation between rosetting and infection rate and intensity in the mosquito. Disruption of rosette formation resulted in lower infection rates and intensities.